Restriction map for
pF1KB8820
(Linear) (Six-base) MAPSORT of: KIBB8820.seq Check: 5780 from: 1 to: 1152
KIBB8820.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 988 1152
Size: 988 164
AlwNI CAG_nnn'CTG
Cuts at: 0 966 1152
Size: 966 186
AvaI C'yCGr_G
Cuts at: 0 470 1152
Size: 470 682
AvrII C'CTAG_G
Cuts at: 0 648 1152
Size: 648 504
BanI G'GyrC_C
Cuts at: 0 998 1005 1152
Size: 998 7 147
Fragments arranged by size:
998 147 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 132 144 324 1152
Size: 132 12 180 828
Fragments arranged by size:
828 180 132 12
BbvCI CC'TCA_GC
Cuts at: 0 181 463 718 1152
Size: 181 282 255 434
Fragments arranged by size:
434 282 255 181
BglI GCCn_nnn'nGGC
Cuts at: 0 231 1152
Size: 231 921
BglII A'GATC_T
Cuts at: 0 245 1152
Size: 245 907
BlpI GC'TnA_GC
Cuts at: 0 446 1152
Size: 446 706
BmrI ACTGGGnnnn_n'
Cuts at: 0 142 1152
Size: 142 1010
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 865 927 1152
Size: 451 414 62 225
Fragments arranged by size:
451 414 225 62
Bpu10I CC'TnA_GC
Cuts at: 0 181 463 718 1152
Size: 181 282 255 434
Fragments arranged by size:
434 282 255 181
BsaAI yAC'GTr
Cuts at: 0 222 1152
Size: 222 930
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 30 1152
Size: 30 1122
BsiHKAI G_wGCw'C
Cuts at: 0 364 1152
Size: 364 788
BsmBI CGTCTCn'nnnn_
Cuts at: 0 528 1152
Size: 528 624
Bsp1286I G_dGCh'C
Cuts at: 0 364 1152
Size: 364 788
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 839 1152
Size: 839 313
BsrGI T'GTAC_A
Cuts at: 0 546 1152
Size: 546 606
BstYI r'GATC_y
Cuts at: 0 245 1152
Size: 245 907
BtgI C'CryG_G
Cuts at: 0 314 1152
Size: 314 838
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 882 1152
Size: 882 270
BtsI GCAGTG_nn'
Cuts at: 0 8 125 292 909 1013 1152
Size: 8 117 167 617 104 139
Fragments arranged by size:
617 167 139 117 104 8
DraI TTT'AAA
Cuts at: 0 746 1152
Size: 746 406
EaeI y'GGCC_r
Cuts at: 0 2 263 518 1152
Size: 2 261 255 634
Fragments arranged by size:
634 261 255 2
EarI CTCTTCn'nnn_
Cuts at: 0 628 1152
Size: 628 524
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 249 946 1152
Size: 249 697 206
Fragments arranged by size:
697 249 206
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 865 927 988 1152
Size: 451 414 62 61 164
Fragments arranged by size:
451 414 164 62 61
EcoNI CCTnn'n_nnAGG
Cuts at: 0 456 1152
Size: 456 696
EcoO109I rG'GnC_Cy
Cuts at: 0 467 1152
Size: 467 685
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1090 1122 1152
Size: 1090 32 30
Fragments arranged by size:
1090 32 30
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 806 1152
Size: 296 510 346
Fragments arranged by size:
510 346 296
MscI TGG'CCA
Cuts at: 0 265 1152
Size: 265 887
MslI CAynn'nnrTG
Cuts at: 0 313 1152
Size: 313 839
MspA1I CmG'CkG
Cuts at: 0 185 1037 1152
Size: 185 852 115
Fragments arranged by size:
852 185 115
PflMI CCAn_nnn'nTGG
Cuts at: 0 503 1152
Size: 503 649
PpuMI rG'GwC_Cy
Cuts at: 0 467 1152
Size: 467 685
PstI C_TGCA'G
Cuts at: 0 109 759 1152
Size: 109 650 393
Fragments arranged by size:
650 393 109
PvuII CAG'CTG
Cuts at: 0 1037 1152
Size: 1037 115
SapI GCTCTTCn'nnn_
Cuts at: 0 628 1152
Size: 628 524
SfcI C'TryA_G
Cuts at: 0 105 156 755 1152
Size: 105 51 599 397
Fragments arranged by size:
599 397 105 51
SmaI CCC'GGG
Cuts at: 0 472 1152
Size: 472 680
StuI AGG'CCT
Cuts at: 0 1024 1152
Size: 1024 128
StyI C'CwwG_G
Cuts at: 0 566 648 761 1092 1152
Size: 566 82 113 331 60
Fragments arranged by size:
566 331 113 82 60
TatI w'GTAC_w
Cuts at: 0 546 1152
Size: 546 606
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 32 1152
Size: 32 1120
XbaI T'CTAG_A
Cuts at: 0 867 1152
Size: 867 285
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 534 1152
Size: 534 618
XmaI C'CCGG_G
Cuts at: 0 470 1152
Size: 470 682
XmnI GAAnn'nnTTC
Cuts at: 0 622 973 1152
Size: 622 351 179
Fragments arranged by size:
622 351 179
Enzymes that do cut:
AcuI AlwNI AvaI AvrII BanI BbsI BbvCI BglI
BglII BlpI BmrI BpmI Bpu10I BsaAI BsaXI BsiHKAI
BsmBI Bsp1286I BspMI BsrGI BstYI BtgI BtgZI BtsI
DraI EaeI EarI EciI Eco57MI EcoNI EcoO109I Hin4I
MmeI MscI MslI MspA1I PflMI PpuMI PstI PvuII
SapI SfcI SmaI StuI StyI TatI TstI XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI BaeI BamHI BanII BbeI BcgI
BciVI BclI BfrBI Bme1580I BmgBI BmtI BplI BpuEI
BsaI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII DrdI EagI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TaqII Tth111I XhoI
ZraI