Restriction map for
pF1KB8822
(Linear) (Six-base) MAPSORT of: KIBB8822.seq Check: 2323 from: 1 to: 1188
KIBB8822.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 257 508 1188
Size: 257 251 680
Fragments arranged by size:
680 257 251
Acc65I G'GTAC_C
Cuts at: 0 483 1188
Size: 483 705
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 1188
Size: 202 986
AgeI A'CCGG_T
Cuts at: 0 262 1188
Size: 262 926
AlwNI CAG_nnn'CTG
Cuts at: 0 633 720 798 1188
Size: 633 87 78 390
Fragments arranged by size:
633 390 87 78
ApaI G_GGCC'C
Cuts at: 0 1166 1188
Size: 1166 22
ApoI r'AATT_y
Cuts at: 0 937 1188
Size: 937 251
AvaI C'yCGr_G
Cuts at: 0 1043 1188
Size: 1043 145
BanI G'GyrC_C
Cuts at: 0 100 265 483 1188
Size: 100 165 218 705
Fragments arranged by size:
705 218 165 100
BanII G_rGCy'C
Cuts at: 0 293 572 921 1166 1188
Size: 293 279 349 245 22
Fragments arranged by size:
349 293 279 245 22
BlpI GC'TnA_GC
Cuts at: 0 571 848 1188
Size: 571 277 340
Fragments arranged by size:
571 340 277
Bme1580I G_kGCm'C
Cuts at: 0 1166 1188
Size: 1166 22
BmrI ACTGGGnnnn_n'
Cuts at: 0 355 743 1188
Size: 355 388 445
Fragments arranged by size:
445 388 355
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 857 1188
Size: 857 331
Bpu10I CC'TnA_GC
Cuts at: 0 374 988 1188
Size: 374 614 200
Fragments arranged by size:
614 374 200
BsaI GGTCTCn'nnnn_
Cuts at: 0 818 1188
Size: 818 370
BsaHI Gr'CG_yC
Cuts at: 0 17 1188
Size: 17 1171
BsaWI w'CCGG_w
Cuts at: 0 262 921 1188
Size: 262 659 267
Fragments arranged by size:
659 267 262
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 341 371 1188
Size: 341 30 817
Fragments arranged by size:
817 341 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 190 283 349 1188
Size: 190 93 66 839
Fragments arranged by size:
839 190 93 66
BseYI C'CCAG_C
Cuts at: 0 48 74 893 1188
Size: 48 26 819 295
Fragments arranged by size:
819 295 48 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 192 235 480 1188
Size: 192 43 245 708
Fragments arranged by size:
708 245 192 43
BsiHKAI G_wGCw'C
Cuts at: 0 119 430 921 1106 1188
Size: 119 311 491 185 82
Fragments arranged by size:
491 311 185 119 82
Bsp1286I G_dGCh'C
Cuts at: 0 119 293 430 572 921 1106 1166 1188
Size: 119 174 137 142 349 185 60 22
Fragments arranged by size:
349 185 174 142 137 119 60 22
BspEI T'CCGG_A
Cuts at: 0 921 1188
Size: 921 267
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 176 257 508 719 1188
Size: 176 81 251 211 469
Fragments arranged by size:
469 251 211 176 81
BsrDI GCAATG_nn'
Cuts at: 0 117 362 1188
Size: 117 245 826
Fragments arranged by size:
826 245 117
BsrFI r'CCGG_y
Cuts at: 0 262 524 1188
Size: 262 262 664
Fragments arranged by size:
664 262 262
BstXI CCAn_nnnn'nTGG
Cuts at: 0 546 678 1141 1188
Size: 546 132 463 47
Fragments arranged by size:
546 463 132 47
BtgI C'CryG_G
Cuts at: 0 104 1188
Size: 104 1084
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 324 1188
Size: 324 864
BtsI GCAGTG_nn'
Cuts at: 0 582 1188
Size: 582 606
DraI TTT'AAA
Cuts at: 0 1154 1188
Size: 1154 34
EaeI y'GGCC_r
Cuts at: 0 134 446 680 1188
Size: 134 312 234 508
Fragments arranged by size:
508 312 234 134
EarI CTCTTCn'nnn_
Cuts at: 0 909 1188
Size: 909 279
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1120 1188
Size: 1120 68
EcoICRI GAG'CTC
Cuts at: 0 919 1188
Size: 919 269
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 857 1188
Size: 202 655 331
Fragments arranged by size:
655 331 202
EcoNI CCTnn'n_nnAGG
Cuts at: 0 276 1188
Size: 276 912
EcoO109I rG'GnC_Cy
Cuts at: 0 745 1162 1163 1188
Size: 745 417 1 25
Fragments arranged by size:
745 417 25 1
FseI GG_CCGG'CC
Cuts at: 0 528 1188
Size: 528 660
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 341 373 815 847 916 948 1188
Size: 341 32 442 32 69 32 240
Fragments arranged by size:
442 341 240 69 32 32 32
HindIII A'AGCT_T
Cuts at: 0 376 1188
Size: 376 812
KpnI G_GTAC'C
Cuts at: 0 487 1188
Size: 487 701
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 695 1188
Size: 695 493
MscI TGG'CCA
Cuts at: 0 136 448 682 1188
Size: 136 312 234 506
Fragments arranged by size:
506 312 234 136
MslI CAynn'nnrTG
Cuts at: 0 544 1188
Size: 544 644
NaeI GCC'GGC
Cuts at: 0 526 1188
Size: 526 662
NdeI CA'TA_TG
Cuts at: 0 1140 1188
Size: 1140 48
NgoMIV G'CCGG_C
Cuts at: 0 524 1188
Size: 524 664
NspI r_CATG'y
Cuts at: 0 1059 1188
Size: 1059 129
PflMI CCAn_nnn'nTGG
Cuts at: 0 720 798 1188
Size: 720 78 390
Fragments arranged by size:
720 390 78
PfoI T'CCnGG_A
Cuts at: 0 713 1188
Size: 713 475
PpuMI rG'GwC_Cy
Cuts at: 0 745 1188
Size: 745 443
PspOMI G'GGCC_C
Cuts at: 0 1162 1188
Size: 1162 26
PstI C_TGCA'G
Cuts at: 0 280 505 1188
Size: 280 225 683
Fragments arranged by size:
683 280 225
SacI G_AGCT'C
Cuts at: 0 921 1188
Size: 921 267
SapI GCTCTTCn'nnn_
Cuts at: 0 909 1188
Size: 909 279
SfcI C'TryA_G
Cuts at: 0 276 488 501 1188
Size: 276 212 13 687
Fragments arranged by size:
687 276 212 13
SgrAI Cr'CCGG_yG
Cuts at: 0 262 1188
Size: 262 926
SmaI CCC'GGG
Cuts at: 0 1045 1188
Size: 1045 143
StyI C'CwwG_G
Cuts at: 0 131 401 1188
Size: 131 270 787
Fragments arranged by size:
787 270 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 805 1188
Size: 805 383
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 186 543 795 1188
Size: 186 357 252 393
Fragments arranged by size:
393 357 252 186
XmaI C'CCGG_G
Cuts at: 0 1043 1188
Size: 1043 145
Enzymes that do cut:
AarI Acc65I AcuI AgeI AlwNI ApaI ApoI AvaI
BanI BanII BlpI Bme1580I BmrI BpmI Bpu10I BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspEI BspMI BsrDI BsrFI BstXI BtgI BtgZI BtsI
DraI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FseI Hin4I HindIII KpnI MmeI MscI MslI NaeI
NdeI NgoMIV NspI PflMI PfoI PpuMI PspOMI PstI
SacI SapI SfcI SgrAI SmaI StyI TaqII XcmI
XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AhdI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BmgBI BmtI BplI BpuEI BsaAI BsaBI
BsiEI BsiWI BsmI BsmBI BspHI BsrBI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I ClaI
CspCI DraIII DrdI EagI EcoRI EcoRV FalI FspI
FspAI HaeII HincII HpaI KasI MfeI MluI MspA1I
NarI NcoI NheI NotI NruI NsiI PacI PasI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI TstI Tth111I XbaI
XhoI XmnI ZraI