Restriction map for
pF1KB8823
(Linear) (Six-base) MAPSORT of: KIBB8823.seq Check: 1047 from: 1 to: 1212
KIBB8823.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 896 1212
Size: 896 316
AccI GT'mk_AC
Cuts at: 0 446 1212
Size: 446 766
AflIII A'CryG_T
Cuts at: 0 407 1212
Size: 407 805
AleI CACnn'nnGTG
Cuts at: 0 49 1212
Size: 49 1163
AlwNI CAG_nnn'CTG
Cuts at: 0 638 1212
Size: 638 574
ApaI G_GGCC'C
Cuts at: 0 592 645 1212
Size: 592 53 567
Fragments arranged by size:
592 567 53
BanI G'GyrC_C
Cuts at: 0 615 761 1212
Size: 615 146 451
Fragments arranged by size:
615 451 146
BanII G_rGCy'C
Cuts at: 0 140 592 599 645 1212
Size: 140 452 7 46 567
Fragments arranged by size:
567 452 140 46 7
BbeI G_GCGC'C
Cuts at: 0 765 1212
Size: 765 447
BbsI GAAGACnn'nnnn_
Cuts at: 0 699 1212
Size: 699 513
BglI GCCn_nnn'nGGC
Cuts at: 0 790 1212
Size: 790 422
BlpI GC'TnA_GC
Cuts at: 0 139 1212
Size: 139 1073
Bme1580I G_kGCm'C
Cuts at: 0 592 645 1042 1212
Size: 592 53 397 170
Fragments arranged by size:
592 397 170 53
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 1212
Size: 352 860
Bpu10I CC'TnA_GC
Cuts at: 0 243 593 1130 1141 1212
Size: 243 350 537 11 71
Fragments arranged by size:
537 350 243 71 11
BsaAI yAC'GTr
Cuts at: 0 132 789 1212
Size: 132 657 423
Fragments arranged by size:
657 423 132
BsaHI Gr'CG_yC
Cuts at: 0 762 1212
Size: 762 450
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 907 937 1212
Size: 907 30 275
Fragments arranged by size:
907 275 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1041 1212
Size: 1041 171
BseYI C'CCAG_C
Cuts at: 0 13 17 1070 1207 1212
Size: 13 4 1053 137 5
Fragments arranged by size:
1053 137 13 5 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 542 916 1212
Size: 542 374 296
Fragments arranged by size:
542 374 296
BsiEI CG_ry'CG
Cuts at: 0 450 1212
Size: 450 762
BsiHKAI G_wGCw'C
Cuts at: 0 140 151 1212
Size: 140 11 1061
Fragments arranged by size:
1061 140 11
BsmI GAATG_Cn'
Cuts at: 0 1002 1074 1212
Size: 1002 72 138
Fragments arranged by size:
1002 138 72
Bsp1286I G_dGCh'C
Cuts at: 0 140 151 592 599 645 1042 1212
Size: 140 11 441 7 46 397 170
Fragments arranged by size:
441 397 170 140 46 11 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 896 1212
Size: 896 316
BsrFI r'CCGG_y
Cuts at: 0 728 758 986 1212
Size: 728 30 228 226
Fragments arranged by size:
728 228 226 30
BstAPI GCAn_nnn'nTGC
Cuts at: 0 504 1212
Size: 504 708
BstEII G'GTnAC_C
Cuts at: 0 42 1212
Size: 42 1170
BstXI CCAn_nnnn'nTGG
Cuts at: 0 159 192 672 1212
Size: 159 33 480 540
Fragments arranged by size:
540 480 159 33
BstYI r'GATC_y
Cuts at: 0 74 1212
Size: 74 1138
Bsu36I CC'TnA_GG
Cuts at: 0 560 1019 1212
Size: 560 459 193
Fragments arranged by size:
560 459 193
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 273 1212
Size: 273 939
BtsI GCAGTG_nn'
Cuts at: 0 56 765 844 870 1212
Size: 56 709 79 26 342
Fragments arranged by size:
709 342 79 56 26
DraIII CAC_nnn'GTG
Cuts at: 0 504 1212
Size: 504 708
DrdI GACnn_nn'nnGTC
Cuts at: 0 454 1212
Size: 454 758
EaeI y'GGCC_r
Cuts at: 0 182 284 542 791 1212
Size: 182 102 258 249 421
Fragments arranged by size:
421 258 249 182 102
EarI CTCTTCn'nnn_
Cuts at: 0 109 613 1059 1212
Size: 109 504 446 153
Fragments arranged by size:
504 446 153 109
EcoICRI GAG'CTC
Cuts at: 0 138 1212
Size: 138 1074
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 1212
Size: 352 860
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1018 1212
Size: 1018 194
EcoO109I rG'GnC_Cy
Cuts at: 0 22 534 588 589 642 854 1212
Size: 22 512 54 1 53 212 358
Fragments arranged by size:
512 358 212 54 53 22 1
HaeII r_GCGC'y
Cuts at: 0 473 765 1212
Size: 473 292 447
Fragments arranged by size:
473 447 292
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 907 939 1212
Size: 907 32 273
Fragments arranged by size:
907 273 32
HincII GTy'rAC
Cuts at: 0 231 405 447 1212
Size: 231 174 42 765
Fragments arranged by size:
765 231 174 42
KasI G'GCGC_C
Cuts at: 0 761 1212
Size: 761 451
MscI TGG'CCA
Cuts at: 0 184 286 1212
Size: 184 102 926
Fragments arranged by size:
926 184 102
MslI CAynn'nnrTG
Cuts at: 0 49 190 1212
Size: 49 141 1022
Fragments arranged by size:
1022 141 49
MspA1I CmG'CkG
Cuts at: 0 17 555 579 1212
Size: 17 538 24 633
Fragments arranged by size:
633 538 24 17
NaeI GCC'GGC
Cuts at: 0 730 760 988 1212
Size: 730 30 228 224
Fragments arranged by size:
730 228 224 30
NarI GG'CG_CC
Cuts at: 0 762 1212
Size: 762 450
NgoMIV G'CCGG_C
Cuts at: 0 728 758 986 1212
Size: 728 30 228 226
Fragments arranged by size:
728 228 226 30
NspI r_CATG'y
Cuts at: 0 411 1046 1212
Size: 411 635 166
Fragments arranged by size:
635 411 166
PciI A'CATG_T
Cuts at: 0 407 1212
Size: 407 805
PflMI CCAn_nnn'nTGG
Cuts at: 0 582 1212
Size: 582 630
PmlI CAC'GTG
Cuts at: 0 132 1212
Size: 132 1080
PpuMI rG'GwC_Cy
Cuts at: 0 534 854 1212
Size: 534 320 358
Fragments arranged by size:
534 358 320
PspOMI G'GGCC_C
Cuts at: 0 588 641 1212
Size: 588 53 571
Fragments arranged by size:
588 571 53
PstI C_TGCA'G
Cuts at: 0 1116 1212
Size: 1116 96
PvuII CAG'CTG
Cuts at: 0 17 579 1212
Size: 17 562 633
Fragments arranged by size:
633 562 17
SacI G_AGCT'C
Cuts at: 0 140 1212
Size: 140 1072
SalI G'TCGA_C
Cuts at: 0 445 1212
Size: 445 767
SanDI GG'GwC_CC
Cuts at: 0 854 1212
Size: 854 358
SexAI A'CCwGG_T
Cuts at: 0 878 1212
Size: 878 334
SfcI C'TryA_G
Cuts at: 0 1001 1112 1212
Size: 1001 111 100
Fragments arranged by size:
1001 111 100
SfoI GGC'GCC
Cuts at: 0 763 1212
Size: 763 449
StyI C'CwwG_G
Cuts at: 0 66 1031 1091 1212
Size: 66 965 60 121
Fragments arranged by size:
965 121 66 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 103 1084 1212
Size: 103 981 128
Fragments arranged by size:
981 128 103
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 294 669 1212
Size: 294 375 543
Fragments arranged by size:
543 375 294
Enzymes that do cut:
AarI AccI AflIII AleI AlwNI ApaI BanI BanII
BbeI BbsI BglI BlpI Bme1580I BpmI Bpu10I BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspMI BsrFI BstAPI BstEII BstXI BstYI Bsu36I
BtgZI BtsI DraIII DrdI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I HincII KasI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PflMI PmlI
PpuMI PspOMI PstI PvuII SacI SalI SanDI SexAI
SfcI SfoI StyI TaqII XcmI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI
BglII BmgBI BmrI BmtI BplI BpuEI BsaI BsaBI
BsaWI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BssSI BstBI BstZ17I BtgI ClaI CspCI DraI
EagI EciI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI MmeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SapI SbfI ScaI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI