Restriction map for
pF1KB8828
(Linear) (Six-base) MAPSORT of: KIBB8828.seq Check: 476 from: 1 to: 1302
KIBB8828.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 482 1302
Size: 482 820
AatII G_ACGT'C
Cuts at: 0 558 1302
Size: 558 744
Acc65I G'GTAC_C
Cuts at: 0 28 1302
Size: 28 1274
AlwNI CAG_nnn'CTG
Cuts at: 0 871 882 1302
Size: 871 11 420
Fragments arranged by size:
871 420 11
AvaI C'yCGr_G
Cuts at: 0 500 1302
Size: 500 802
BanI G'GyrC_C
Cuts at: 0 28 1131 1302
Size: 28 1103 171
Fragments arranged by size:
1103 171 28
BanII G_rGCy'C
Cuts at: 0 529 856 1011 1302
Size: 529 327 155 291
Fragments arranged by size:
529 327 291 155
BbsI GAAGACnn'nnnn_
Cuts at: 0 325 1302
Size: 325 977
BbvCI CC'TCA_GC
Cuts at: 0 343 1302
Size: 343 959
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 620 654 1302
Size: 620 34 648
Fragments arranged by size:
648 620 34
BglI GCCn_nnn'nGGC
Cuts at: 0 82 1189 1302
Size: 82 1107 113
Fragments arranged by size:
1107 113 82
Bme1580I G_kGCm'C
Cuts at: 0 901 1302
Size: 901 401
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 995 1025 1302
Size: 74 921 30 277
Fragments arranged by size:
921 277 74 30
Bpu10I CC'TnA_GC
Cuts at: 0 343 739 850 1302
Size: 343 396 111 452
Fragments arranged by size:
452 396 343 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 156 415 1302
Size: 156 259 887
Fragments arranged by size:
887 259 156
BsaHI Gr'CG_yC
Cuts at: 0 555 1302
Size: 555 747
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 27 57 1227 1257 1302
Size: 27 30 1170 30 45
Fragments arranged by size:
1170 45 30 30 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 418 464 524 736 739 769 1302
Size: 85 333 46 60 212 3 30 533
Fragments arranged by size:
533 333 212 85 60 46 30 3
BseYI C'CCAG_C
Cuts at: 0 980 1302
Size: 980 322
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 729 845 1019 1302
Size: 729 116 174 283
Fragments arranged by size:
729 283 174 116
BsiHKAI G_wGCw'C
Cuts at: 0 856 1011 1302
Size: 856 155 291
Fragments arranged by size:
856 291 155
BsmI GAATG_Cn'
Cuts at: 0 735 1302
Size: 735 567
Bsp1286I G_dGCh'C
Cuts at: 0 529 856 901 1011 1302
Size: 529 327 45 110 291
Fragments arranged by size:
529 327 291 110 45
BspHI T'CATG_A
Cuts at: 0 558 1302
Size: 558 744
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 482 759 788 1302
Size: 482 277 29 514
Fragments arranged by size:
514 482 277 29
BsrDI GCAATG_nn'
Cuts at: 0 923 1302
Size: 923 379
BsrFI r'CCGG_y
Cuts at: 0 378 626 1302
Size: 378 248 676
Fragments arranged by size:
676 378 248
BstAPI GCAn_nnn'nTGC
Cuts at: 0 164 1042 1302
Size: 164 878 260
Fragments arranged by size:
878 260 164
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1248 1302
Size: 1248 54
BtgI C'CryG_G
Cuts at: 0 285 337 918 1148 1247 1302
Size: 285 52 581 230 99 55
Fragments arranged by size:
581 285 230 99 55 52
BtsI GCAGTG_nn'
Cuts at: 0 102 857 1302
Size: 102 755 445
Fragments arranged by size:
755 445 102
ClaI AT'CG_AT
Cuts at: 0 1198 1302
Size: 1198 104
EaeI y'GGCC_r
Cuts at: 0 785 1289 1302
Size: 785 504 13
Fragments arranged by size:
785 504 13
EcoICRI GAG'CTC
Cuts at: 0 854 1009 1302
Size: 854 155 293
Fragments arranged by size:
854 293 155
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 995 1025 1302
Size: 74 921 30 277
Fragments arranged by size:
921 277 74 30
EcoO109I rG'GnC_Cy
Cuts at: 0 49 408 1302
Size: 49 359 894
Fragments arranged by size:
894 359 49
EcoRV GAT'ATC
Cuts at: 0 172 573 1302
Size: 172 401 729
Fragments arranged by size:
729 401 172
FspI TGC'GCA
Cuts at: 0 1282 1302
Size: 1282 20
FspAI rTGC'GCAy
Cuts at: 0 1282 1302
Size: 1282 20
KpnI G_GTAC'C
Cuts at: 0 32 1302
Size: 32 1270
MfeI C'AATT_G
Cuts at: 0 1115 1302
Size: 1115 187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 1302
Size: 224 1078
MscI TGG'CCA
Cuts at: 0 787 1302
Size: 787 515
MslI CAynn'nnrTG
Cuts at: 0 1246 1291 1302
Size: 1246 45 11
Fragments arranged by size:
1246 45 11
MspA1I CmG'CkG
Cuts at: 0 41 361 1015 1099 1302
Size: 41 320 654 84 203
Fragments arranged by size:
654 320 203 84 41
NaeI GCC'GGC
Cuts at: 0 628 1302
Size: 628 674
NcoI C'CATG_G
Cuts at: 0 285 918 1148 1247 1302
Size: 285 633 230 99 55
Fragments arranged by size:
633 285 230 99 55
NdeI CA'TA_TG
Cuts at: 0 672 1302
Size: 672 630
NgoMIV G'CCGG_C
Cuts at: 0 626 1302
Size: 626 676
NspI r_CATG'y
Cuts at: 0 1281 1302
Size: 1281 21
PasI CC'CwG_GG
Cuts at: 0 1122 1302
Size: 1122 180
PflMI CCAn_nnn'nTGG
Cuts at: 0 1128 1212 1302
Size: 1128 84 90
Fragments arranged by size:
1128 90 84
PpuMI rG'GwC_Cy
Cuts at: 0 408 1302
Size: 408 894
PvuII CAG'CTG
Cuts at: 0 361 1015 1099 1302
Size: 361 654 84 203
Fragments arranged by size:
654 361 203 84
SacI G_AGCT'C
Cuts at: 0 856 1011 1302
Size: 856 155 291
Fragments arranged by size:
856 291 155
SfcI C'TryA_G
Cuts at: 0 123 961 1302
Size: 123 838 341
Fragments arranged by size:
838 341 123
SgrAI Cr'CCGG_yG
Cuts at: 0 626 1302
Size: 626 676
SmlI C'TyrA_G
Cuts at: 0 135 430 1302
Size: 135 295 872
Fragments arranged by size:
872 295 135
SphI G_CATG'C
Cuts at: 0 1281 1302
Size: 1281 21
StyI C'CwwG_G
Cuts at: 0 285 293 366 836 918 1148 1211 1247
Size: 285 8 73 470 82 230 63 36
Cuts at: 1247 1302
Size: 55
Fragments arranged by size:
470 285 230 82 73 63 55 36
8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 194 1302
Size: 194 1108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 59 1227 1259 1302
Size: 27 32 1168 32 43
Fragments arranged by size:
1168 43 32 32 27
Tth111I GACn'n_nGTC
Cuts at: 0 554 1302
Size: 554 748
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1155 1245 1302
Size: 1155 90 57
Fragments arranged by size:
1155 90 57
ZraI GAC'GTC
Cuts at: 0 556 1302
Size: 556 746
Enzymes that do cut:
AarI AatII Acc65I AlwNI AvaI BanI BanII BbsI
BbvCI BcgI BglI Bme1580I BpmI Bpu10I BpuEI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrDI BsrFI BstAPI BstXI BtgI BtsI ClaI
EaeI EcoICRI Eco57MI EcoO109I EcoRV FspI FspAI KpnI
MfeI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NspI PasI PflMI PpuMI PvuII SacI SfcI
SgrAI SmlI SphI StyI TaqII TstI Tth111I XcmI
ZraI
Enzymes that do not cut:
AccI AclI AcuI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BciVI BclI BfrBI
BglII BlpI BmgBI BmrI BmtI BplI BsaI BsaAI
BsaBI BsaWI BsiEI BsiWI BsmBI BspEI BsrBI BsrGI
BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI
CspCI DraI DraIII DrdI EagI EarI EciI EcoNI
EcoRI FalI FseI HaeII Hin4I HincII HindIII HpaI
KasI MluI NarI NheI NotI NruI NsiI PacI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SmaI
SnaBI SpeI SrfI SspI StuI SwaI TatI XbaI
XhoI XmaI XmnI