Restriction map for
pF1KB8841
(Linear) (Six-base) MAPSORT of: KIBB8841.seq Check: 145 from: 1 to: 1425
KIBB8841.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 647 714 833 1425
Size: 103 544 67 119 592
Fragments arranged by size:
592 544 119 103 67
AleI CACnn'nnGTG
Cuts at: 0 1292 1425
Size: 1292 133
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1296 1425
Size: 1296 129
AlwNI CAG_nnn'CTG
Cuts at: 0 75 1395 1425
Size: 75 1320 30
Fragments arranged by size:
1320 75 30
ApoI r'AATT_y
Cuts at: 0 50 462 595 1425
Size: 50 412 133 830
Fragments arranged by size:
830 412 133 50
AvaI C'yCGr_G
Cuts at: 0 359 1234 1425
Size: 359 875 191
Fragments arranged by size:
875 359 191
BamHI G'GATC_C
Cuts at: 0 477 1073 1425
Size: 477 596 352
Fragments arranged by size:
596 477 352
BanI G'GyrC_C
Cuts at: 0 507 797 1425
Size: 507 290 628
Fragments arranged by size:
628 507 290
BanII G_rGCy'C
Cuts at: 0 182 458 1425
Size: 182 276 967
Fragments arranged by size:
967 276 182
BciVI GTATCCnnnnn_n'
Cuts at: 0 815 933 1425
Size: 815 118 492
Fragments arranged by size:
815 492 118
BglI GCCn_nnn'nGGC
Cuts at: 0 223 1399 1425
Size: 223 1176 26
Fragments arranged by size:
1176 223 26
Bme1580I G_kGCm'C
Cuts at: 0 800 1425
Size: 800 625
BmrI ACTGGGnnnn_n'
Cuts at: 0 1062 1425
Size: 1062 363
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 298 335 595 649 919 925 1425
Size: 82 216 37 260 54 270 6 500
Fragments arranged by size:
500 270 260 216 82 54 37 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 568 1025 1425
Size: 47 521 457 400
Fragments arranged by size:
521 457 400 47
BsaBI GATnn'nnATC
Cuts at: 0 1193 1425
Size: 1193 232
BsaHI Gr'CG_yC
Cuts at: 0 346 1425
Size: 346 1079
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 917 947 1425
Size: 917 30 478
Fragments arranged by size:
917 478 30
BseYI C'CCAG_C
Cuts at: 0 210 297 324 1131 1425
Size: 210 87 27 807 294
Fragments arranged by size:
807 294 210 87 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 1425
Size: 88 1337
BsiHKAI G_wGCw'C
Cuts at: 0 200 935 1425
Size: 200 735 490
Fragments arranged by size:
735 490 200
BsmBI CGTCTCn'nnnn_
Cuts at: 0 353 1425
Size: 353 1072
Bsp1286I G_dGCh'C
Cuts at: 0 182 200 458 800 935 1425
Size: 182 18 258 342 135 490
Fragments arranged by size:
490 342 258 182 135 18
BsrDI GCAATG_nn'
Cuts at: 0 916 1034 1425
Size: 916 118 391
Fragments arranged by size:
916 391 118
BstXI CCAn_nnnn'nTGG
Cuts at: 0 218 305 1425
Size: 218 87 1120
Fragments arranged by size:
1120 218 87
BstYI r'GATC_y
Cuts at: 0 107 276 392 477 1073 1425
Size: 107 169 116 85 596 352
Fragments arranged by size:
596 352 169 116 107 85
BtsI GCAGTG_nn'
Cuts at: 0 76 1425
Size: 76 1349
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 52 87 1425
Size: 52 35 1338
Fragments arranged by size:
1338 52 35
DraIII CAC_nnn'GTG
Cuts at: 0 1163 1425
Size: 1163 262
DrdI GACnn_nn'nnGTC
Cuts at: 0 1238 1425
Size: 1238 187
EarI CTCTTCn'nnn_
Cuts at: 0 1172 1425
Size: 1172 253
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 103 298 335 595 647 649 714
Size: 82 21 195 37 260 52 2 65
Cuts at: 714 833 919 925 1425
Size: 119 86 6 500
Fragments arranged by size:
500 260 195 119 86 82 65 52
37 21 6 2
EcoO109I rG'GnC_Cy
Cuts at: 0 321 418 678 1392 1425
Size: 321 97 260 714 33
Fragments arranged by size:
714 321 260 97 33
EcoRI G'AATT_C
Cuts at: 0 462 595 1425
Size: 462 133 830
Fragments arranged by size:
830 462 133
EcoRV GAT'ATC
Cuts at: 0 888 1425
Size: 888 537
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1108 1425
Size: 1108 317
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1102 1134 1425
Size: 1102 32 291
Fragments arranged by size:
1102 291 32
HindIII A'AGCT_T
Cuts at: 0 748 1425
Size: 748 677
MfeI C'AATT_G
Cuts at: 0 840 1425
Size: 840 585
MslI CAynn'nnrTG
Cuts at: 0 928 1292 1425
Size: 928 364 133
Fragments arranged by size:
928 364 133
MspA1I CmG'CkG
Cuts at: 0 771 1249 1425
Size: 771 478 176
Fragments arranged by size:
771 478 176
PasI CC'CwG_GG
Cuts at: 0 287 1425
Size: 287 1138
PfoI T'CCnGG_A
Cuts at: 0 314 935 1425
Size: 314 621 490
Fragments arranged by size:
621 490 314
PstI C_TGCA'G
Cuts at: 0 531 1221 1400 1425
Size: 531 690 179 25
Fragments arranged by size:
690 531 179 25
PvuII CAG'CTG
Cuts at: 0 771 1249 1425
Size: 771 478 176
Fragments arranged by size:
771 478 176
SbfI CC_TGCA'GG
Cuts at: 0 1400 1425
Size: 1400 25
SfcI C'TryA_G
Cuts at: 0 527 698 1217 1396 1425
Size: 527 171 519 179 29
Fragments arranged by size:
527 519 179 171 29
SmaI CCC'GGG
Cuts at: 0 361 1425
Size: 361 1064
SmlI C'TyrA_G
Cuts at: 0 62 547 1040 1425
Size: 62 485 493 385
Fragments arranged by size:
493 485 385 62
SspI AAT'ATT
Cuts at: 0 489 1425
Size: 489 936
StuI AGG'CCT
Cuts at: 0 1417 1425
Size: 1417 8
StyI C'CwwG_G
Cuts at: 0 137 173 999 1425
Size: 137 36 826 426
Fragments arranged by size:
826 426 137 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 445 1425
Size: 445 980
XmaI C'CCGG_G
Cuts at: 0 359 1425
Size: 359 1066
Enzymes that do cut:
AcuI AleI AlfI AlwNI ApoI AvaI BamHI BanI
BanII BciVI BglI Bme1580I BmrI BpmI BpuEI BsaBI
BsaHI BsaXI BseYI BsgI BsiHKAI BsmBI Bsp1286I BsrDI
BstXI BstYI BtsI CspCI DraIII DrdI EarI Eco57MI
EcoO109I EcoRI EcoRV FalI Hin4I HindIII MfeI MslI
MspA1I PasI PfoI PstI PvuII SbfI SfcI SmaI
SmlI SspI StuI StyI TaqII XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BbeI BbsI BbvCI BcgI BclI BfrBI
BglII BlpI BmgBI BmtI BplI Bpu10I BsaI BsaAI
BsaWI BseRI BsiEI BsiWI BsmI BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgI BtgZI ClaI DraI EaeI EagI
EciI EcoICRI EcoNI FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MluI MmeI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SwaI TatI TstI
Tth111I XbaI XcmI XhoI XmnI ZraI