Restriction map for
pF1KB8843
(Linear) (Six-base) MAPSORT of: KIBB8843.seq Check: 9864 from: 1 to: 1488
KIBB8843.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AatII G_ACGT'C
Cuts at: 0 867 1488
Size: 867 621
AccI GT'mk_AC
Cuts at: 0 846 1488
Size: 846 642
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 806 962 1488
Size: 806 156 526
Fragments arranged by size:
806 526 156
AfeI AGC'GCT
Cuts at: 0 808 1488
Size: 808 680
AgeI A'CCGG_T
Cuts at: 0 841 1488
Size: 841 647
AlwNI CAG_nnn'CTG
Cuts at: 0 996 1488
Size: 996 492
ApaI G_GGCC'C
Cuts at: 0 144 1488
Size: 144 1344
ApoI r'AATT_y
Cuts at: 0 643 820 1488
Size: 643 177 668
Fragments arranged by size:
668 643 177
AvaI C'yCGr_G
Cuts at: 0 531 1224 1488
Size: 531 693 264
Fragments arranged by size:
693 531 264
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 404 437 1488
Size: 404 33 1051
Fragments arranged by size:
1051 404 33
BanI G'GyrC_C
Cuts at: 0 82 268 331 454 1338 1371 1488
Size: 82 186 63 123 884 33 117
Fragments arranged by size:
884 186 123 117 82 63 33
BanII G_rGCy'C
Cuts at: 0 144 746 1225 1488
Size: 144 602 479 263
Fragments arranged by size:
602 479 263 144
BbeI G_GCGC'C
Cuts at: 0 86 272 335 1342 1375 1488
Size: 86 186 63 1007 33 113
Fragments arranged by size:
1007 186 113 86 63 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 469 1488
Size: 469 1019
BbvCI CC'TCA_GC
Cuts at: 0 985 1488
Size: 985 503
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 361 395 1488
Size: 361 34 1093
Fragments arranged by size:
1093 361 34
BglI GCCn_nnn'nGGC
Cuts at: 0 139 277 403 1126 1488
Size: 139 138 126 723 362
Fragments arranged by size:
723 362 139 138 126
BlpI GC'TnA_GC
Cuts at: 0 1180 1488
Size: 1180 308
Bme1580I G_kGCm'C
Cuts at: 0 144 1488
Size: 144 1344
BmgBI CAC'GTC
Cuts at: 0 545 1488
Size: 545 943
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 211 596 1096 1100 1231 1488
Size: 94 117 385 500 4 131 257
Fragments arranged by size:
500 385 257 131 117 94 4
Bpu10I CC'TnA_GC
Cuts at: 0 656 959 985 1488
Size: 656 303 26 503
Fragments arranged by size:
656 503 303 26
BsaI GGTCTCn'nnnn_
Cuts at: 0 367 786 1488
Size: 367 419 702
Fragments arranged by size:
702 419 367
BsaAI yAC'GTr
Cuts at: 0 90 1488
Size: 90 1398
BsaHI Gr'CG_yC
Cuts at: 0 83 269 332 395 864 1339 1372 1410
Size: 83 186 63 63 469 475 33 38
Cuts at: 1410 1488
Size: 78
Fragments arranged by size:
475 469 186 83 78 63 63 38
33
BsaWI w'CCGG_w
Cuts at: 0 841 1488
Size: 841 647
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 170 200 1081 1098 1111 1128 1182 1212
Size: 170 30 881 17 13 17 54 30
Cuts at: 1212 1488
Size: 276
Fragments arranged by size:
881 276 170 54 30 30 17 17
13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 593 596 637 755 1488
Size: 593 3 41 118 733
Fragments arranged by size:
733 593 118 41 3
BseYI C'CCAG_C
Cuts at: 0 1443 1488
Size: 1443 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 525 1488
Size: 525 963
BsiEI CG_ry'CG
Cuts at: 0 846 1264 1488
Size: 846 418 224
Fragments arranged by size:
846 418 224
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1401 1488
Size: 1401 87
Bsp1286I G_dGCh'C
Cuts at: 0 144 746 1225 1488
Size: 144 602 479 263
Fragments arranged by size:
602 479 263 144
BsrBI CCG'CTC
Cuts at: 0 690 1215 1488
Size: 690 525 273
Fragments arranged by size:
690 525 273
BsrFI r'CCGG_y
Cuts at: 0 841 1120 1488
Size: 841 279 368
Fragments arranged by size:
841 368 279
BstXI CCAn_nnnn'nTGG
Cuts at: 0 390 1488
Size: 390 1098
BstYI r'GATC_y
Cuts at: 0 1433 1488
Size: 1433 55
Bsu36I CC'TnA_GG
Cuts at: 0 450 884 1488
Size: 450 434 604
Fragments arranged by size:
604 450 434
BtgI C'CryG_G
Cuts at: 0 272 297 360 898 1488
Size: 272 25 63 538 590
Fragments arranged by size:
590 538 272 63 25
BtsI GCAGTG_nn'
Cuts at: 0 385 1488
Size: 385 1103
DrdI GACnn_nn'nnGTC
Cuts at: 0 313 1488
Size: 313 1175
EaeI y'GGCC_r
Cuts at: 0 1261 1488
Size: 1261 227
EagI C'GGCC_G
Cuts at: 0 1261 1488
Size: 1261 227
EarI CTCTTCn'nnn_
Cuts at: 0 29 614 656 1488
Size: 29 585 42 832
Fragments arranged by size:
832 585 42 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 919 1209 1426 1488
Size: 919 290 217 62
Fragments arranged by size:
919 290 217 62
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 211 596 806 962 1096 1100 1231
Size: 94 117 385 210 156 134 4 131
Cuts at: 1231 1488
Size: 257
Fragments arranged by size:
385 257 210 156 134 131 117 94
4
EcoO109I rG'GnC_Cy
Cuts at: 0 507 750 880 888 925 1488
Size: 507 243 130 8 37 563
Fragments arranged by size:
563 507 243 130 37 8
EcoRI G'AATT_C
Cuts at: 0 643 820 1488
Size: 643 177 668
Fragments arranged by size:
668 643 177
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 72 1488
Size: 72 1416
FspI TGC'GCA
Cuts at: 0 107 1488
Size: 107 1381
HaeII r_GCGC'y
Cuts at: 0 86 241 272 335 810 1083 1097 1342
Size: 86 155 31 63 475 273 14 245
Cuts at: 1342 1375 1488
Size: 33 113
Fragments arranged by size:
475 273 245 155 113 86 63 33
31 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 658 690 785 817 1098 1130 1182 1214
Size: 658 32 95 32 281 32 52 32
Cuts at: 1214 1234 1266 1488
Size: 20 32 222
Fragments arranged by size:
658 281 222 95 52 32 32 32
32 32 20
HincII GTy'rAC
Cuts at: 0 847 1106 1488
Size: 847 259 382
Fragments arranged by size:
847 382 259
KasI G'GCGC_C
Cuts at: 0 82 268 331 1338 1371 1488
Size: 82 186 63 1007 33 117
Fragments arranged by size:
1007 186 117 82 63 33
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 585 1488
Size: 585 903
MspA1I CmG'CkG
Cuts at: 0 274 362 574 1488
Size: 274 88 212 914
Fragments arranged by size:
914 274 212 88
NaeI GCC'GGC
Cuts at: 0 1122 1488
Size: 1122 366
NarI GG'CG_CC
Cuts at: 0 83 269 332 1339 1372 1488
Size: 83 186 63 1007 33 116
Fragments arranged by size:
1007 186 116 83 63 33
NcoI C'CATG_G
Cuts at: 0 898 1488
Size: 898 590
NgoMIV G'CCGG_C
Cuts at: 0 1120 1488
Size: 1120 368
PasI CC'CwG_GG
Cuts at: 0 496 746 1488
Size: 496 250 742
Fragments arranged by size:
742 496 250
PflMI CCAn_nnn'nTGG
Cuts at: 0 354 1488
Size: 354 1134
PfoI T'CCnGG_A
Cuts at: 0 188 1238 1488
Size: 188 1050 250
Fragments arranged by size:
1050 250 188
PpuMI rG'GwC_Cy
Cuts at: 0 507 888 925 1488
Size: 507 381 37 563
Fragments arranged by size:
563 507 381 37
PspOMI G'GGCC_C
Cuts at: 0 140 1488
Size: 140 1348
PstI C_TGCA'G
Cuts at: 0 569 1488
Size: 569 919
SacII CC_GC'GG
Cuts at: 0 275 363 1488
Size: 275 88 1125
Fragments arranged by size:
1125 275 88
SalI G'TCGA_C
Cuts at: 0 845 1488
Size: 845 643
SapI GCTCTTCn'nnn_
Cuts at: 0 29 1488
Size: 29 1459
SfcI C'TryA_G
Cuts at: 0 565 1488
Size: 565 923
SfoI GGC'GCC
Cuts at: 0 84 270 333 1340 1373 1488
Size: 84 186 63 1007 33 115
Fragments arranged by size:
1007 186 115 84 63 33
SmaI CCC'GGG
Cuts at: 0 533 1488
Size: 533 955
StyI C'CwwG_G
Cuts at: 0 407 898 1488
Size: 407 491 590
Fragments arranged by size:
590 491 407
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 236 795 1488
Size: 236 559 693
Fragments arranged by size:
693 559 236
Tth111I GACn'n_nGTC
Cuts at: 0 851 1488
Size: 851 637
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1398 1462 1488
Size: 1398 64 26
Fragments arranged by size:
1398 64 26
XmaI C'CCGG_G
Cuts at: 0 531 1488
Size: 531 957
ZraI GAC'GTC
Cuts at: 0 865 1488
Size: 865 623
Enzymes that do cut:
AatII AccI AcuI AfeI AgeI AlwNI ApaI ApoI
AvaI BaeI BanI BanII BbeI BbsI BbvCI BcgI
BglI BlpI Bme1580I BmgBI BpmI Bpu10I BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsmBI
Bsp1286I BsrBI BsrFI BstXI BstYI Bsu36I BtgI BtsI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI MmeI MspA1I
NaeI NarI NcoI NgoMIV PasI PflMI PfoI PpuMI
PspOMI PstI SacII SalI SapI SfcI SfoI SmaI
StyI TaqII Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AflIII AhdI AleI AlfI
AloI ApaLI AscI AseI AsiSI AvrII BamHI BciVI
BclI BfrBI BglII BmrI BmtI BplI BpuEI BsaBI
BsiHKAI BsiWI BsmI BspEI BspHI BspMI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI
CspCI DraI DraIII EcoICRI EcoNI EcoRV FseI FspAI
HindIII HpaI KpnI MfeI MluI MscI MslI NdeI
NheI NotI NruI NsiI NspI PacI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacI SanDI SbfI ScaI SexAI SfiI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI TstI XbaI XhoI XmnI