Restriction map for pF1KB8848
 (Linear) (Six-base) MAPSORT of: KIBB8848.seq  Check: 5601  from: 1  to: 201



KIBB8848.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139     201 
   Size:        139      62
 
AfeI AGC'GCT

Cuts at:      0     124     201 
   Size:        124      77
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      21      55      79     113     201 
   Size:         21      34      24      34      88

  Fragments arranged by size: 

                  88      34      34      24      21
 
BclI T'GATC_A

Cuts at:      0       2     201 
   Size:          2     199
 
BsaAI yAC'GTr

Cuts at:      0     156     201 
   Size:        156      45
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139     201 
   Size:        139      62
 
HaeII r_GCGC'y

Cuts at:      0     126     201 
   Size:        126      75
 
PasI CC'CwG_GG

Cuts at:      0     111     201 
   Size:        111      90
 
PmlI CAC'GTG

Cuts at:      0     156     201 
   Size:        156      45
 
PstI C_TGCA'G

Cuts at:      0      77     201 
   Size:         77     124
 
SfcI C'TryA_G

Cuts at:      0      73     201 
   Size:         73     128
 
TatI w'GTAC_w

Cuts at:      0      83     201 
   Size:         83     118
 

 Enzymes that do cut:

     AcuI     AfeI     BcgI     BclI    BsaAI  Eco57MI    HaeII     PasI
     PmlI     PstI     SfcI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI    PflMI     PfoI     PmeI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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