(Linear) (Six-base) MAPSORT of: KIBB8848.seq Check: 5601 from: 1 to: 201
KIBB8848.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 201
Size: 139 62
AfeI AGC'GCT
Cuts at: 0 124 201
Size: 124 77
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 21 55 79 113 201
Size: 21 34 24 34 88
Fragments arranged by size:
88 34 34 24 21
BclI T'GATC_A
Cuts at: 0 2 201
Size: 2 199
BsaAI yAC'GTr
Cuts at: 0 156 201
Size: 156 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 201
Size: 139 62
HaeII r_GCGC'y
Cuts at: 0 126 201
Size: 126 75
PasI CC'CwG_GG
Cuts at: 0 111 201
Size: 111 90
PmlI CAC'GTG
Cuts at: 0 156 201
Size: 156 45
PstI C_TGCA'G
Cuts at: 0 77 201
Size: 77 124
SfcI C'TryA_G
Cuts at: 0 73 201
Size: 73 128
TatI w'GTAC_w
Cuts at: 0 83 201
Size: 83 118
Enzymes that do cut:
AcuI AfeI BcgI BclI BsaAI Eco57MI HaeII PasI
PmlI PstI SfcI TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI