Restriction map for pF1KB8849
 (Linear) (Six-base) MAPSORT of: KIBB8849.seq  Check: 2884  from: 1  to: 213



KIBB8849.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

AlwNI CAG_nnn'CTG

Cuts at:      0      57     213 
   Size:         57     156
 
AseI AT'TA_AT

Cuts at:      0     178     213 
   Size:        178      35
 
BmgBI CAC'GTC

Cuts at:      0     126     213 
   Size:        126      87
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     213 
   Size:        142      71
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      57     213 
   Size:         57     156
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      84     213 
   Size:         84     129
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     124     213 
   Size:        124      89
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     213 
   Size:        142      71
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      40     213 
   Size:         40     173
 
HincII GTy'rAC

Cuts at:      0       6     213 
   Size:          6     207
 
PasI CC'CwG_GG

Cuts at:      0      84     213 
   Size:         84     129
 
PfoI T'CCnGG_A

Cuts at:      0     158     213 
   Size:        158      55
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     160     213 
   Size:        160      53
 
TatI w'GTAC_w

Cuts at:      0      62     213 
   Size:         62     151
 
XmnI GAAnn'nnTTC

Cuts at:      0      94     213 
   Size:         94     119
 

 Enzymes that do cut:

    AlwNI     AseI    BmgBI     BpmI   BstAPI    BstXI     DrdI  Eco57MI
     FalI   HincII     PasI     PfoI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I     BmrI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI
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