(Linear) (Six-base) MAPSORT of: KIBB8850.seq  Check: 8475  from: 1  to: 213



KIBB8850.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:29  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     152     213 
   Size:        152      61
 
AflII C'TTAA_G

Cuts at:      0      55     213 
   Size:         55     158
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     146     213 
   Size:        146      67
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     178     208     213 
   Size:        178      30       5

  Fragments arranged by size: 

                 178      30       5
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      42     213 
   Size:         42     171
 
EaeI y'GGCC_r

Cuts at:      0       2     213 
   Size:          2     211
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     152     213 
   Size:        152      61
 
MscI TGG'CCA

Cuts at:      0       4     213 
   Size:          4     209
 
MspA1I CmG'CkG

Cuts at:      0     100     213 
   Size:        100     113
 
NspI r_CATG'y

Cuts at:      0     133     213 
   Size:        133      80
 
PstI C_TGCA'G

Cuts at:      0     105     213 
   Size:        105     108
 
PvuII CAG'CTG

Cuts at:      0     100     213 
   Size:        100     113
 
SfcI C'TryA_G

Cuts at:      0     101     213 
   Size:        101     112
 
SmlI C'TyrA_G

Cuts at:      0      55     213 
   Size:         55     158
 
StyI C'CwwG_G

Cuts at:      0      92     134     213 
   Size:         92      42      79

  Fragments arranged by size: 

                  92      79      42
 
XmnI GAAnn'nnTTC

Cuts at:      0     196     213 
   Size:        196      17
 

 Enzymes that do cut:

     AcuI    AflII     BbsI    BsaXI    BstXI     EaeI  Eco57MI     MscI
   MspA1I     NspI     PstI    PvuII     SfcI     SmlI     StyI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI