(Linear) (Six-base) MAPSORT of: KIBB8851.seq  Check: 8528  from: 1  to: 219



KIBB8851.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:29  ..

AclI AA'CG_TT

Cuts at:      0     138     219 
   Size:        138      81
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     131     219 
   Size:        131      88
 
ApoI r'AATT_y

Cuts at:      0     167     219 
   Size:        167      52
 
BamHI G'GATC_C

Cuts at:      0     150     219 
   Size:        150      69
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     182     219 
   Size:        182      37
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     164     219 
   Size:        164      55
 
BstYI r'GATC_y

Cuts at:      0     150     219 
   Size:        150      69
 
DraI TTT'AAA

Cuts at:      0     192     219 
   Size:        192      27
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     131     164     219 
   Size:        131      33      55

  Fragments arranged by size: 

                 131      55      33
 
EcoRI G'AATT_C

Cuts at:      0     167     219 
   Size:        167      52
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     204     219 
   Size:        204      15
 
MspA1I CmG'CkG

Cuts at:      0     206     219 
   Size:        206      13
 
PvuII CAG'CTG

Cuts at:      0     206     219 
   Size:        206      13
 

 Enzymes that do cut:

     AclI     AcuI     ApoI    BamHI     BbsI     BpmI    BstYI     DraI
  Eco57MI    EcoRI     FalI   MspA1I    PvuII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRV     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI