(Linear) (Six-base) MAPSORT of: KIBB8852.seq  Check: 9222  from: 1  to: 225



KIBB8852.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:29  ..

AflII C'TTAA_G

Cuts at:      0      67     225 
   Size:         67     158
 
AleI CACnn'nnGTG

Cuts at:      0     146     169     225 
   Size:        146      23      56

  Fragments arranged by size: 

                 146      56      23
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     190     222     225 
   Size:        190      32       3

  Fragments arranged by size: 

                 190      32       3
 
BamHI G'GATC_C

Cuts at:      0     153     225 
   Size:        153      72
 
Bme1580I G_kGCm'C

Cuts at:      0     176     225 
   Size:        176      49
 
BsaI GGTCTCn'nnnn_

Cuts at:      0       4     225 
   Size:          4     221
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     190     220     225 
   Size:        190      30       5

  Fragments arranged by size: 

                 190      30       5
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     211     225 
   Size:        211      14
 
BsiHKAI G_wGCw'C

Cuts at:      0     219     225 
   Size:        219       6
 
Bsp1286I G_dGCh'C

Cuts at:      0     176     219     225 
   Size:        176      43       6

  Fragments arranged by size: 

                 176      43       6
 
BstYI r'GATC_y

Cuts at:      0     153     225 
   Size:        153      72
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     225 
   Size:        225
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     225 
   Size:         70     155
 
MslI CAynn'nnrTG

Cuts at:      0     146     169     225 
   Size:        146      23      56

  Fragments arranged by size: 

                 146      56      23
 
PfoI T'CCnGG_A

Cuts at:      0     194     225 
   Size:        194      31
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     190     222     225 
   Size:        190      32       3

  Fragments arranged by size: 

                 190      32       3
 
SmlI C'TyrA_G

Cuts at:      0      67     225 
   Size:         67     158
 
StyI C'CwwG_G

Cuts at:      0     104     225 
   Size:        104     121
 
XmnI GAAnn'nnTTC

Cuts at:      0     190     205     208     225 
   Size:        190      15       3      17

  Fragments arranged by size: 

                 190      17      15       3
 

 Enzymes that do cut:

    AflII     AleI     AloI    BamHI Bme1580I     BsaI    BsaXI    BseRI
  BsiHKAI Bsp1286I    BstYI     FalI     MmeI     MslI     PfoI     PpiI
     SmlI     StyI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AhdI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI
     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI