(Linear) (Six-base) MAPSORT of: KIBB8853.seq  Check: 5404  from: 1  to: 228



KIBB8853.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      23     228 
   Size:         23     205
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     118     228 
   Size:        118     110
 
BsrDI GCAATG_nn'

Cuts at:      0     111     228 
   Size:        111     117
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      23     228 
   Size:         23     205
 
EcoRV GAT'ATC

Cuts at:      0      75     138     228 
   Size:         75      63      90

  Fragments arranged by size: 

                  90      75      63
 
NdeI CA'TA_TG

Cuts at:      0      21     228 
   Size:         21     207
 
PasI CC'CwG_GG

Cuts at:      0     201     228 
   Size:        201      27
 
SfcI C'TryA_G

Cuts at:      0     179     228 
   Size:        179      49
 

 Enzymes that do cut:

     AcuI    BspMI    BsrDI  Eco57MI    EcoRV     NdeI     PasI     SfcI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI