(Linear) (Six-base) MAPSORT of: KIBB8855.seq  Check: 1373  from: 1  to: 261



KIBB8855.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      71     261 
   Size:         71     190
 
AhdI GACnn_n'nnGTC

Cuts at:      0      60     261 
   Size:         60     201
 
BmgBI CAC'GTC

Cuts at:      0      57     261 
   Size:         57     204
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     198     261 
   Size:        198      63
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     261 
   Size:        193      68
 
BsmI GAATG_Cn'

Cuts at:      0     114     261 
   Size:        114     147
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      71     193     261 
   Size:         71     122      68

  Fragments arranged by size: 

                 122      71      68
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      46      78     136     168     261 
   Size:         46      32      58      32      93

  Fragments arranged by size: 

                  93      58      46      32      32
 
MfeI C'AATT_G

Cuts at:      0     232     261 
   Size:        232      29
 
MspA1I CmG'CkG

Cuts at:      0     136     261 
   Size:        136     125
 
PvuII CAG'CTG

Cuts at:      0     136     261 
   Size:        136     125
 

 Enzymes that do cut:

     AcuI     AhdI    BmgBI     BplI     BpmI     BsmI  Eco57MI    Hin4I
     MfeI   MspA1I    PvuII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I     BmrI     BmtI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI