(Linear) (Six-base) MAPSORT of: KIBB8856.seq  Check: 3732  from: 1  to: 270



KIBB8856.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:50  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     270 
   Size:        121     149
 
BanII G_rGCy'C

Cuts at:      0     125     270 
   Size:        125     145
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     179     270 
   Size:        179      91
 
Bpu10I CC'TnA_GC

Cuts at:      0      85     270 
   Size:         85     185
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     247     270 
   Size:        247      23
 
BsaWI w'CCGG_w

Cuts at:      0     182     270 
   Size:        182      88
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      40      70     270 
   Size:         40      30     200

  Fragments arranged by size: 

                 200      40      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      22     270 
   Size:         22     248
 
BsiHKAI G_wGCw'C

Cuts at:      0     125     270 
   Size:        125     145
 
Bsp1286I G_dGCh'C

Cuts at:      0     125     270 
   Size:        125     145
 
BssSI C'ACGA_G

Cuts at:      0      34     270 
   Size:         34     236
 
EaeI y'GGCC_r

Cuts at:      0      38     270 
   Size:         38     232
 
EcoICRI GAG'CTC

Cuts at:      0     123     270 
   Size:        123     147
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     270 
   Size:        121     149
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      16     270 
   Size:         16     254
 
EcoO109I rG'GnC_Cy

Cuts at:      0     233     270 
   Size:        233      37
 
MscI TGG'CCA

Cuts at:      0      40     270 
   Size:         40     230
 
NspI r_CATG'y

Cuts at:      0       8     270 
   Size:          8     262
 
PfoI T'CCnGG_A

Cuts at:      0     209     270 
   Size:        209      61
 
PstI C_TGCA'G

Cuts at:      0     146     270 
   Size:        146     124
 
SacI G_AGCT'C

Cuts at:      0     125     270 
   Size:        125     145
 
ScaI AGT'ACT

Cuts at:      0      55     270 
   Size:         55     215
 
SfcI C'TryA_G

Cuts at:      0     142     270 
   Size:        142     128
 
SmlI C'TyrA_G

Cuts at:      0     262     270 
   Size:        262       8
 
SphI G_CATG'C

Cuts at:      0       8     270 
   Size:          8     262
 
StyI C'CwwG_G

Cuts at:      0     236     270 
   Size:        236      34
 
TatI w'GTAC_w

Cuts at:      0      53     270 
   Size:         53     217
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      40      72     270 
   Size:         40      32     198

  Fragments arranged by size: 

                 198      40      32
 

 Enzymes that do cut:

     AcuI    BanII     BbsI   Bpu10I    BpuEI    BsaWI    BsaXI    BseRI
  BsiHKAI Bsp1286I    BssSI     EaeI  EcoICRI  Eco57MI    EcoNI EcoO109I
     MscI     NspI     PfoI     PstI     SacI     ScaI     SfcI     SmlI
     SphI     StyI     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
     BsaI    BsaAI    BsaBI    BsaHI    BseYI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EarI     EciI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI
    TaqII  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI