(Linear) (Six-base) MAPSORT of: KIBB8858.seq  Check: 3275  from: 1  to: 276



KIBB8858.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

AccI GT'mk_AC

Cuts at:      0     206     276 
   Size:        206      70
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     265     276 
   Size:        118     147      11

  Fragments arranged by size: 

                 147     118      11
 
ApoI r'AATT_y

Cuts at:      0     217     276 
   Size:        217      59
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     276 
   Size:         43     233
 
BsaBI GATnn'nnATC

Cuts at:      0     188     276 
   Size:        188      88
 
BsrDI GCAATG_nn'

Cuts at:      0     236     276 
   Size:        236      40
 
EarI CTCTTCn'nnn_

Cuts at:      0       8     276 
   Size:          8     268
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     118     265     276 
   Size:         43      75     147      11

  Fragments arranged by size: 

                 147      75      43      11
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      85     276 
   Size:         85     191
 
EcoRI G'AATT_C

Cuts at:      0     217     276 
   Size:        217      59
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      67      99     276 
   Size:         67      32     177

  Fragments arranged by size: 

                 177      67      32
 
HincII GTy'rAC

Cuts at:      0     207     276 
   Size:        207      69
 
MspA1I CmG'CkG

Cuts at:      0     105     276 
   Size:        105     171
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     180     276 
   Size:        180      96
 
PvuII CAG'CTG

Cuts at:      0     105     276 
   Size:        105     171
 
SalI G'TCGA_C

Cuts at:      0     205     276 
   Size:        205      71
 
SapI GCTCTTCn'nnn_

Cuts at:      0       8     276 
   Size:          8     268
 
SspI AAT'ATT

Cuts at:      0     170     276 
   Size:        170     106
 
StuI AGG'CCT

Cuts at:      0     179     276 
   Size:        179      97
 
StyI C'CwwG_G

Cuts at:      0     174     276 
   Size:        174     102
 

 Enzymes that do cut:

     AccI     AcuI     ApoI     BpmI    BsaBI    BsrDI     EarI  Eco57MI
    EcoNI    EcoRI    Hin4I   HincII   MspA1I    PflMI    PvuII     SalI
     SapI     SspI     StuI     StyI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII    SanDI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI