(Linear) (Six-base) MAPSORT of: KIBB8862.seq  Check: 7830  from: 1  to: 303



KIBB8862.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

AflIII A'CryG_T

Cuts at:      0      44     303 
   Size:         44     259
 
Bme1580I G_kGCm'C

Cuts at:      0     154     303 
   Size:        154     149
 
BmgBI CAC'GTC

Cuts at:      0      67     303 
   Size:         67     236
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      26     303 
   Size:         26     277
 
BseYI C'CCAG_C

Cuts at:      0     234     303 
   Size:        234      69
 
Bsp1286I G_dGCh'C

Cuts at:      0     154     303 
   Size:        154     149
 
BtgI C'CryG_G

Cuts at:      0     270     303 
   Size:        270      33
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     132     303 
   Size:        132     171
 
HaeII r_GCGC'y

Cuts at:      0     283     303 
   Size:        283      20
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     211     243     303 
   Size:        211      32      60

  Fragments arranged by size: 

                 211      60      32
 
NcoI C'CATG_G

Cuts at:      0     270     303 
   Size:        270      33
 
NspI r_CATG'y

Cuts at:      0      48     303 
   Size:         48     255
 
PciI A'CATG_T

Cuts at:      0      44     303 
   Size:         44     259
 
SmlI C'TyrA_G

Cuts at:      0      11     303 
   Size:         11     292
 
StyI C'CwwG_G

Cuts at:      0     270     303 
   Size:        270      33
 

 Enzymes that do cut:

   AflIII Bme1580I    BmgBI     BplI    BseYI Bsp1286I     BtgI     FalI
    HaeII    Hin4I     NcoI     NspI     PciI     SmlI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI     BmrI     BmtI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI