(Linear) (Six-base) MAPSORT of: KIBB8866.seq  Check: 5174  from: 1  to: 345



KIBB8866.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

ApaLI G'TGCA_C

Cuts at:      0     184     345 
   Size:        184     161
 
BanII G_rGCy'C

Cuts at:      0     210     345 
   Size:        210     135
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     263     345 
   Size:        263      82
 
BfrBI ATG'CAT

Cuts at:      0     117     345 
   Size:        117     228
 
BglII A'GATC_T

Cuts at:      0      91     345 
   Size:         91     254
 
Bme1580I G_kGCm'C

Cuts at:      0     188     345 
   Size:        188     157
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     200     345 
   Size:        200     145
 
BsiHKAI G_wGCw'C

Cuts at:      0     188     210     345 
   Size:        188      22     135

  Fragments arranged by size: 

                 188     135      22
 
Bsp1286I G_dGCh'C

Cuts at:      0     188     210     345 
   Size:        188      22     135

  Fragments arranged by size: 

                 188     135      22
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      15     345 
   Size:         15     330
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     183     345 
   Size:        183     162
 
BstYI r'GATC_y

Cuts at:      0      91     345 
   Size:         91     254
 
EarI CTCTTCn'nnn_

Cuts at:      0      82     345 
   Size:         82     263
 
EcoICRI GAG'CTC

Cuts at:      0     208     345 
   Size:        208     137
 
EcoO109I rG'GnC_Cy

Cuts at:      0     242     345 
   Size:        242     103
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      61      93     345 
   Size:         61      32     252

  Fragments arranged by size: 

                 252      61      32
 
NsiI A_TGCA'T

Cuts at:      0     119     345 
   Size:        119     226
 
PstI C_TGCA'G

Cuts at:      0     179     345 
   Size:        179     166
 
SacI G_AGCT'C

Cuts at:      0     210     345 
   Size:        210     135
 
SfcI C'TryA_G

Cuts at:      0     175     345 
   Size:        175     170
 
StyI C'CwwG_G

Cuts at:      0     201     345 
   Size:        201     144
 
Tth111I GACn'n_nGTC

Cuts at:      0     262     345 
   Size:        262      83
 

 Enzymes that do cut:

    ApaLI    BanII     BbsI    BfrBI    BglII Bme1580I    BseRI  BsiHKAI
 Bsp1286I    BspMI   BstAPI    BstYI     EarI  EcoICRI EcoO109I    Hin4I
     NsiI     PstI     SacI     SfcI     StyI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI    BbvCI     BcgI    BciVI     BclI     BglI     BlpI
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI