(Linear) (Six-base) MAPSORT of: KIBB8869.seq  Check: 3873  from: 1  to: 399



KIBB8869.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

Acc65I G'GTAC_C

Cuts at:      0     262     399 
   Size:        262     137
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      77     209     399 
   Size:         77     132     190

  Fragments arranged by size: 

                 190     132      77
 
AflIII A'CryG_T

Cuts at:      0     324     399 
   Size:        324      75
 
AlwNI CAG_nnn'CTG

Cuts at:      0      18     399 
   Size:         18     381
 
ApoI r'AATT_y

Cuts at:      0     156     196     399 
   Size:        156      40     203

  Fragments arranged by size: 

                 203     156      40
 
AseI AT'TA_AT

Cuts at:      0     239     399 
   Size:        239     160
 
AvaI C'yCGr_G

Cuts at:      0     257     399 
   Size:        257     142
 
AvrII C'CTAG_G

Cuts at:      0     150     399 
   Size:        150     249
 
BanI G'GyrC_C

Cuts at:      0     262     399 
   Size:        262     137
 
BanII G_rGCy'C

Cuts at:      0     308     399 
   Size:        308      91
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      49      76     146     399 
   Size:         49      27      70     253

  Fragments arranged by size: 

                 253      70      49      27
 
BclI T'GATC_A

Cuts at:      0     173     399 
   Size:        173     226
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     399 
   Size:         40     359
 
BsiHKAI G_wGCw'C

Cuts at:      0     308     399 
   Size:        308      91
 
BsmI GAATG_Cn'

Cuts at:      0     248     399 
   Size:        248     151
 
Bsp1286I G_dGCh'C

Cuts at:      0     308     399 
   Size:        308      91
 
BspHI T'CATG_A

Cuts at:      0     176     308     399 
   Size:        176     132      91

  Fragments arranged by size: 

                 176     132      91
 
BssSI C'ACGA_G

Cuts at:      0     133     399 
   Size:        133     266
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      97     399 
   Size:         97     302
 
DraIII CAC_nnn'GTG

Cuts at:      0     117     399 
   Size:        117     282
 
EcoICRI GAG'CTC

Cuts at:      0     306     399 
   Size:        306      93
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40      77     209     399 
   Size:         40      37     132     190

  Fragments arranged by size: 

                 190     132      40      37
 
KpnI G_GTAC'C

Cuts at:      0     266     399 
   Size:        266     133
 
MslI CAynn'nnrTG

Cuts at:      0     175     399 
   Size:        175     224
 
MspA1I CmG'CkG

Cuts at:      0      97     274     399 
   Size:         97     177     125

  Fragments arranged by size: 

                 177     125      97
 
NspI r_CATG'y

Cuts at:      0     328     399 
   Size:        328      71
 
PciI A'CATG_T

Cuts at:      0     324     399 
   Size:        324      75
 
PspXI vC'TCGA_Gb

Cuts at:      0     257     399 
   Size:        257     142
 
PvuII CAG'CTG

Cuts at:      0      97     274     399 
   Size:         97     177     125

  Fragments arranged by size: 

                 177     125      97
 
SacI G_AGCT'C

Cuts at:      0     308     399 
   Size:        308      91
 
SmlI C'TyrA_G

Cuts at:      0     257     399 
   Size:        257     142
 
StuI AGG'CCT

Cuts at:      0     296     399 
   Size:        296     103
 
StyI C'CwwG_G

Cuts at:      0     150     399 
   Size:        150     249
 
XhoI C'TCGA_G

Cuts at:      0     257     399 
   Size:        257     142
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII    AlwNI     ApoI     AseI     AvaI    AvrII
     BanI    BanII     BbsI     BclI     BpmI  BsiHKAI     BsmI Bsp1286I
    BspHI    BssSI    BstXI   DraIII  EcoICRI  Eco57MI     KpnI     MslI
   MspA1I     NspI     PciI    PspXI    PvuII     SacI     SmlI     StuI
     StyI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI     BaeI
    BamHI     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI
     EaeI     EagI     EarI     EciI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     MfeI     MluI     MmeI     MscI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI     PsrI     PstI     PvuI    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI     ZraI