(Linear) (Six-base) MAPSORT of: KIBB8870.seq  Check: 4486  from: 1  to: 402



KIBB8870.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:50  ..

AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     277     309     402 
   Size:        277      32      93

  Fragments arranged by size: 

                 277      93      32
 
ApoI r'AATT_y

Cuts at:      0     355     402 
   Size:        355      47
 
AvaI C'yCGr_G

Cuts at:      0     277     402 
   Size:        277     125
 
BanI G'GyrC_C

Cuts at:      0     147     402 
   Size:        147     255
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      84     118     402 
   Size:         84      34     284

  Fragments arranged by size: 

                 284      84      34
 
Bme1580I G_kGCm'C

Cuts at:      0     152     402 
   Size:        152     250
 
BmgBI CAC'GTC

Cuts at:      0     201     402 
   Size:        201     201
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     402 
   Size:        121     281
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     267     402 
   Size:        267     135
 
BsaBI GATnn'nnATC

Cuts at:      0     187     402 
   Size:        187     215
 
BsaHI Gr'CG_yC

Cuts at:      0     292     301     402 
   Size:        292       9     101

  Fragments arranged by size: 

                 292     101       9
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     112     142     402 
   Size:        112      30     260

  Fragments arranged by size: 

                 260     112      30
 
BseYI C'CCAG_C

Cuts at:      0     151     402 
   Size:        151     251
 
Bsp1286I G_dGCh'C

Cuts at:      0     152     402 
   Size:        152     250
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     230     402 
   Size:        230     172
 
DraIII CAC_nnn'GTG

Cuts at:      0     397     402 
   Size:        397       5
 
EcoRI G'AATT_C

Cuts at:      0     355     402 
   Size:        355      47
 
EcoRV GAT'ATC

Cuts at:      0     327     402 
   Size:        327      75
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     112     144     358     390     402 
   Size:        112      32     214      32      12

  Fragments arranged by size: 

                 214     112      32      32      12
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      26     402 
   Size:         26     376
 
MspA1I CmG'CkG

Cuts at:      0     306     402 
   Size:        306      96
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      57     402 
   Size:         57     345
 
PfoI T'CCnGG_A

Cuts at:      0      67     295     402 
   Size:         67     228     107

  Fragments arranged by size: 

                 228     107      67
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     109     141     402 
   Size:        109      32     261

  Fragments arranged by size: 

                 261     109      32
 
PstI C_TGCA'G

Cuts at:      0     197     227     402 
   Size:        197      30     175

  Fragments arranged by size: 

                 197     175      30
 
SfcI C'TryA_G

Cuts at:      0     193     223     402 
   Size:        193      30     179

  Fragments arranged by size: 

                 193     179      30
 
SmaI CCC'GGG

Cuts at:      0     279     402 
   Size:        279     123
 
SmlI C'TyrA_G

Cuts at:      0     136     402 
   Size:        136     266
 
XmaI C'CCGG_G

Cuts at:      0     277     402 
   Size:        277     125
 
XmnI GAAnn'nnTTC

Cuts at:      0       7     402 
   Size:          7     395
 

 Enzymes that do cut:

     AloI     ApoI     AvaI     BanI     BcgI Bme1580I    BmgBI    BpuEI
     BsaI    BsaBI    BsaHI    BsaXI    BseYI Bsp1286I    BspMI   DraIII
    EcoRI    EcoRV    Hin4I     MmeI   MspA1I    PflMI     PfoI     PsrI
     PstI     SfcI     SmaI     SmlI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI
     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BsaAI    BsaWI    BseRI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     ZraI