Restriction map for
pF1KB8871
(Linear) (Six-base) MAPSORT of: KIBB8871.seq Check: 5409 from: 1 to: 474
KIBB8871.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 415 474
Size: 415 59
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 474
Size: 96 378
AflII C'TTAA_G
Cuts at: 0 103 474
Size: 103 371
AlwNI CAG_nnn'CTG
Cuts at: 0 285 474
Size: 285 189
AvaI C'yCGr_G
Cuts at: 0 77 474
Size: 77 397
BanII G_rGCy'C
Cuts at: 0 187 401 474
Size: 187 214 73
Fragments arranged by size:
214 187 73
BbsI GAAGACnn'nnnn_
Cuts at: 0 102 474
Size: 102 372
BglI GCCn_nnn'nGGC
Cuts at: 0 382 474
Size: 382 92
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 366 474
Size: 366 108
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 109 453 474
Size: 45 64 344 21
Fragments arranged by size:
344 64 45 21
BsaAI yAC'GTr
Cuts at: 0 456 474
Size: 456 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 385 391 474
Size: 385 6 83
Fragments arranged by size:
385 83 6
BseYI C'CCAG_C
Cuts at: 0 269 289 426 474
Size: 269 20 137 48
Fragments arranged by size:
269 137 48 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 474
Size: 259 215
BsiHKAI G_wGCw'C
Cuts at: 0 146 474
Size: 146 328
BsmI GAATG_Cn'
Cuts at: 0 200 474
Size: 200 274
Bsp1286I G_dGCh'C
Cuts at: 0 146 187 401 474
Size: 146 41 214 73
Fragments arranged by size:
214 146 73 41
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 415 474
Size: 415 59
BstAPI GCAn_nnn'nTGC
Cuts at: 0 282 474
Size: 282 192
Bsu36I CC'TnA_GG
Cuts at: 0 70 474
Size: 70 404
BtgI C'CryG_G
Cuts at: 0 6 47 474
Size: 6 41 427
Fragments arranged by size:
427 41 6
EaeI y'GGCC_r
Cuts at: 0 242 383 474
Size: 242 141 91
Fragments arranged by size:
242 141 91
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 96 109 453 474
Size: 45 51 13 344 21
Fragments arranged by size:
344 51 45 21 13
EcoO109I rG'GnC_Cy
Cuts at: 0 74 375 474
Size: 74 301 99
Fragments arranged by size:
301 99 74
HaeII r_GCGC'y
Cuts at: 0 124 474
Size: 124 350
MscI TGG'CCA
Cuts at: 0 244 474
Size: 244 230
MspA1I CmG'CkG
Cuts at: 0 116 197 273 282 474
Size: 116 81 76 9 192
Fragments arranged by size:
192 116 81 76 9
NspI r_CATG'y
Cuts at: 0 27 474
Size: 27 447
PflMI CCAn_nnn'nTGG
Cuts at: 0 219 474
Size: 219 255
PfoI T'CCnGG_A
Cuts at: 0 29 125 474
Size: 29 96 349
Fragments arranged by size:
349 96 29
PpuMI rG'GwC_Cy
Cuts at: 0 74 474
Size: 74 400
PvuII CAG'CTG
Cuts at: 0 273 282 474
Size: 273 9 192
Fragments arranged by size:
273 192 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 382 474
Size: 382 92
SmaI CCC'GGG
Cuts at: 0 79 474
Size: 79 395
SmlI C'TyrA_G
Cuts at: 0 103 474
Size: 103 371
StyI C'CwwG_G
Cuts at: 0 179 474
Size: 179 295
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 297 474
Size: 297 177
XmaI C'CCGG_G
Cuts at: 0 77 474
Size: 77 397
Enzymes that do cut:
AarI AcuI AflII AlwNI AvaI BanII BbsI BglI
BplI BpmI BsaAI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspMI BstAPI Bsu36I BtgI EaeI Eco57MI EcoO109I
HaeII MscI MspA1I NspI PflMI PfoI PpuMI PvuII
SfiI SmaI SmlI StyI TaqII XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI
BmtI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI
BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI
EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MslI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI TstI
Tth111I XbaI XcmI XhoI XmnI ZraI