(Linear) (Six-base) MAPSORT of: KIBB8880.seq  Check: 1389  from: 1  to: 525



KIBB8880.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

AccI GT'mk_AC

Cuts at:      0     191     525 
   Size:        191     334
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     525 
   Size:        175     350
 
ApoI r'AATT_y

Cuts at:      0      13     525 
   Size:         13     512
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     245     525 
   Size:        245     280
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     330     525 
   Size:        330     195
 
BglII A'GATC_T

Cuts at:      0     488     525 
   Size:        488      37
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     264     525 
   Size:        264     261
 
Bpu10I CC'TnA_GC

Cuts at:      0      38     525 
   Size:         38     487
 
BseYI C'CCAG_C

Cuts at:      0     416     525 
   Size:        416     109
 
BstYI r'GATC_y

Cuts at:      0     488     525 
   Size:        488      37
 
BtsI GCAGTG_nn'

Cuts at:      0      58     194     525 
   Size:         58     136     331

  Fragments arranged by size: 

                 331     136      58
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     525 
   Size:        175     350
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      80     525 
   Size:         80     445
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     180     525 
   Size:        180     345
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      76     222     525 
   Size:         76     146     303

  Fragments arranged by size: 

                 303     146      76
 
PstI C_TGCA'G

Cuts at:      0      53     525 
   Size:         53     472
 
SfcI C'TryA_G

Cuts at:      0      49      61     525 
   Size:         49      12     464

  Fragments arranged by size: 

                 464      49      12
 
XbaI T'CTAG_A

Cuts at:      0     471     525 
   Size:        471      54
 
XmnI GAAnn'nnTTC

Cuts at:      0     290     525 
   Size:        290     235
 

 Enzymes that do cut:

     AccI     AcuI     ApoI     BbsI    BciVI    BglII     BmrI   Bpu10I
    BseYI    BstYI     BtsI  Eco57MI    EcoNI     FalI    PflMI     PstI
     SfcI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI     BlpI Bme1580I
    BmgBI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI EcoO109I    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XcmI     XhoI     XmaI
     ZraI