(Linear) (Six-base) MAPSORT of: KIBB8886.seq  Check: 9767  from: 1  to: 579



KIBB8886.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     432     579 
   Size:        432     147
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     178     210     579 
   Size:        178      32     369

  Fragments arranged by size: 

                 369     178      32
 
ApoI r'AATT_y

Cuts at:      0     165     579 
   Size:        165     414
 
BanII G_rGCy'C

Cuts at:      0     299     579 
   Size:        299     280
 
BbvCI CC'TCA_GC

Cuts at:      0     508     579 
   Size:        508      71
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     111     579 
   Size:        111     468
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     281     450     579 
   Size:        281     169     129

  Fragments arranged by size: 

                 281     169     129
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     579 
   Size:        202     377
 
Bpu10I CC'TnA_GC

Cuts at:      0     508     513     579 
   Size:        508       5      66

  Fragments arranged by size: 

                 508      66       5
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     251     579 
   Size:        251     328
 
BsaAI yAC'GTr

Cuts at:      0     327     579 
   Size:        327     252
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     471     579 
   Size:        471     108
 
Bsp1286I G_dGCh'C

Cuts at:      0     299     579 
   Size:        299     280
 
EaeI y'GGCC_r

Cuts at:      0      35     579 
   Size:         35     544
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     432     579 
   Size:        202     230     147

  Fragments arranged by size: 

                 230     202     147
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     133     579 
   Size:        133     446
 
EcoO109I rG'GnC_Cy

Cuts at:      0     231     579 
   Size:        231     348
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     168     579 
   Size:        168     411
 
PmlI CAC'GTG

Cuts at:      0     327     579 
   Size:        327     252
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     113     145     579 
   Size:        113      32     434

  Fragments arranged by size: 

                 434     113      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     231     579 
   Size:        231     348
 
SmlI C'TyrA_G

Cuts at:      0     266     579 
   Size:        266     313
 
SspI AAT'ATT

Cuts at:      0     143     579 
   Size:        143     436
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      86     579 
   Size:         86     493
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     360     392     548     579 
   Size:        360      32     156      31

  Fragments arranged by size: 

                 360     156      32      31
 

 Enzymes that do cut:

     AcuI     AloI     ApoI    BanII    BbvCI     BmrI     BplI     BpmI
   Bpu10I    BpuEI    BsaAI    BseRI Bsp1286I     EaeI  Eco57MI    EcoNI
 EcoO109I    PflMI     PmlI     PpiI    PpuMI     SmlI     SspI    TaqII
     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EarI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PfoI     PmeI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     StuI     StyI     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI