Restriction map for
pF1KB8891
(Linear) (Six-base) MAPSORT of: KIBB8891.seq Check: 9086 from: 1 to: 633
KIBB8891.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
ApoI r'AATT_y
Cuts at: 0 303 633
Size: 303 330
AvaI C'yCGr_G
Cuts at: 0 85 633
Size: 85 548
BanI G'GyrC_C
Cuts at: 0 90 633
Size: 90 543
BbeI G_GCGC'C
Cuts at: 0 94 633
Size: 94 539
BbsI GAAGACnn'nnnn_
Cuts at: 0 107 633
Size: 107 526
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 525 559 633
Size: 525 34 74
Fragments arranged by size:
525 74 34
BclI T'GATC_A
Cuts at: 0 174 633
Size: 174 459
BglI GCCn_nnn'nGGC
Cuts at: 0 335 633
Size: 335 298
BlpI GC'TnA_GC
Cuts at: 0 139 633
Size: 139 494
BsaHI Gr'CG_yC
Cuts at: 0 91 633
Size: 91 542
BsiEI CG_ry'CG
Cuts at: 0 117 633
Size: 117 516
BsiWI C'GTAC_G
Cuts at: 0 464 633
Size: 464 169
BsmI GAATG_Cn'
Cuts at: 0 606 633
Size: 606 27
BsrBI CCG'CTC
Cuts at: 0 107 270 633
Size: 107 163 363
Fragments arranged by size:
363 163 107
BsrFI r'CCGG_y
Cuts at: 0 70 633
Size: 70 563
BssHII G'CGCG_C
Cuts at: 0 125 633
Size: 125 508
BstEII G'GTnAC_C
Cuts at: 0 230 633
Size: 230 403
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 461 528 633
Size: 461 67 105
Fragments arranged by size:
461 105 67
BtsI GCAGTG_nn'
Cuts at: 0 150 257 633
Size: 150 107 376
Fragments arranged by size:
376 150 107
DraI TTT'AAA
Cuts at: 0 374 633
Size: 374 259
EaeI y'GGCC_r
Cuts at: 0 114 633
Size: 114 519
EagI C'GGCC_G
Cuts at: 0 114 633
Size: 114 519
EarI CTCTTCn'nnn_
Cuts at: 0 258 633
Size: 258 375
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 42 633
Size: 42 591
EcoRI G'AATT_C
Cuts at: 0 303 633
Size: 303 330
HaeII r_GCGC'y
Cuts at: 0 94 633
Size: 94 539
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 547 579 633
Size: 547 32 54
Fragments arranged by size:
547 54 32
KasI G'GCGC_C
Cuts at: 0 90 633
Size: 90 543
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 633
Size: 301 332
MslI CAynn'nnrTG
Cuts at: 0 374 633
Size: 374 259
MspA1I CmG'CkG
Cuts at: 0 63 289 437 454 633
Size: 63 226 148 17 179
Fragments arranged by size:
226 179 148 63 17
NarI GG'CG_CC
Cuts at: 0 91 633
Size: 91 542
NspI r_CATG'y
Cuts at: 0 533 633
Size: 533 100
PshAI GACnn'nnGTC
Cuts at: 0 389 633
Size: 389 244
PstI C_TGCA'G
Cuts at: 0 288 436 633
Size: 288 148 197
Fragments arranged by size:
288 197 148
PvuII CAG'CTG
Cuts at: 0 289 454 633
Size: 289 165 179
Fragments arranged by size:
289 179 165
SexAI A'CCwGG_T
Cuts at: 0 581 633
Size: 581 52
SfcI C'TryA_G
Cuts at: 0 284 432 633
Size: 284 148 201
Fragments arranged by size:
284 201 148
SfoI GGC'GCC
Cuts at: 0 92 633
Size: 92 541
SspI AAT'ATT
Cuts at: 0 476 633
Size: 476 157
StuI AGG'CCT
Cuts at: 0 81 633
Size: 81 552
StyI C'CwwG_G
Cuts at: 0 407 633
Size: 407 226
SwaI ATTT'AAAT
Cuts at: 0 374 633
Size: 374 259
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 34 66 633
Size: 34 32 567
Fragments arranged by size:
567 34 32
XmnI GAAnn'nnTTC
Cuts at: 0 606 633
Size: 606 27
Enzymes that do cut:
ApoI AvaI BanI BbeI BbsI BcgI BclI BglI
BlpI BsaHI BsiEI BsiWI BsmI BsrBI BsrFI BssHII
BstEII BtgZI BtsI DraI EaeI EagI EarI EciI
EcoRI HaeII Hin4I KasI MmeI MslI MspA1I NarI
NspI PshAI PstI PvuII SexAI SfcI SfoI SspI
StuI StyI SwaI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanII
BbvCI BciVI BfrBI BglII Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrDI BsrGI BssSI BstAPI BstBI BstXI
BstYI BstZ17I Bsu36I BtgI ClaI CspCI DraIII DrdI
EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MscI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI TaqII
TatI Tth111I XbaI XcmI XhoI XmaI ZraI