(Linear) (Six-base) MAPSORT of: KIBB8895.seq  Check: 872  from: 1  to: 669



KIBB8895.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

AccI GT'mk_AC

Cuts at:      0     450     669 
   Size:        450     219
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     209     669 
   Size:        209     460
 
AflIII A'CryG_T

Cuts at:      0     503     669 
   Size:        503     166
 
ApoI r'AATT_y

Cuts at:      0     196     472     669 
   Size:        196     276     197

  Fragments arranged by size: 

                 276     197     196
 
AseI AT'TA_AT

Cuts at:      0      58     669 
   Size:         58     611
 
BamHI G'GATC_C

Cuts at:      0     394     669 
   Size:        394     275
 
BanII G_rGCy'C

Cuts at:      0     181     669 
   Size:        181     488
 
BmrI ACTGGGnnnn_n'

Cuts at:      0       6     669 
   Size:          6     663
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     511     541     669 
   Size:        511      30     128

  Fragments arranged by size: 

                 511     128      30
 
Bsp1286I G_dGCh'C

Cuts at:      0     181     669 
   Size:        181     488
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     605     669 
   Size:        605      64
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     391     439     669 
   Size:        391      48     230

  Fragments arranged by size: 

                 391     230      48
 
BstYI r'GATC_y

Cuts at:      0     394     592     669 
   Size:        394     198      77

  Fragments arranged by size: 

                 394     198      77
 
BtsI GCAGTG_nn'

Cuts at:      0     603     669 
   Size:        603      66
 
DraI TTT'AAA

Cuts at:      0     357     669 
   Size:        357     312
 
EaeI y'GGCC_r

Cuts at:      0     146     669 
   Size:        146     523
 
EarI CTCTTCn'nnn_

Cuts at:      0     621     669 
   Size:        621      48
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     209     669 
   Size:        209     460
 
EcoRV GAT'ATC

Cuts at:      0      87     669 
   Size:         87     582
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     150     669 
   Size:        150     519
 
HaeII r_GCGC'y

Cuts at:      0     120     669 
   Size:        120     549
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     603     635     669 
   Size:        603      32      34

  Fragments arranged by size: 

                 603      34      32
 
MscI TGG'CCA

Cuts at:      0     148     669 
   Size:        148     521
 
MslI CAynn'nnrTG

Cuts at:      0      43     669 
   Size:         43     626
 
NdeI CA'TA_TG

Cuts at:      0     219     669 
   Size:        219     450
 
NspI r_CATG'y

Cuts at:      0     507     669 
   Size:        507     162
 
PciI A'CATG_T

Cuts at:      0     503     669 
   Size:        503     166
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     297     329     669 
   Size:        297      32     340

  Fragments arranged by size: 

                 340     297      32
 
PstI C_TGCA'G

Cuts at:      0     610     669 
   Size:        610      59
 
SfcI C'TryA_G

Cuts at:      0     606     669 
   Size:        606      63
 
SpeI A'CTAG_T

Cuts at:      0     283     669 
   Size:        283     386
 
StuI AGG'CCT

Cuts at:      0     133     669 
   Size:        133     536
 
TatI w'GTAC_w

Cuts at:      0     567     669 
   Size:        567     102
 
XmnI GAAnn'nnTTC

Cuts at:      0      59     669 
   Size:         59     610
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     ApoI     AseI    BamHI    BanII     BmrI
    BsaXI Bsp1286I   BstAPI    BstXI    BstYI     BtsI     DraI     EaeI
     EarI  Eco57MI    EcoRV     FalI    HaeII    Hin4I     MscI     MslI
     NdeI     NspI     PciI     PsrI     PstI     SfcI     SpeI     StuI
     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI     BanI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SphI     SrfI     SspI
     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI