Restriction map for
pF1KB8902
(Linear) (Six-base) MAPSORT of: KIBB8902.seq Check: 2855 from: 1 to: 702
KIBB8902.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 702
Size: 311 391
AfeI AGC'GCT
Cuts at: 0 376 702
Size: 376 326
AleI CACnn'nnGTG
Cuts at: 0 403 702
Size: 403 299
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 409 441 702
Size: 409 32 261
Fragments arranged by size:
409 261 32
ApaI G_GGCC'C
Cuts at: 0 239 465 702
Size: 239 226 237
Fragments arranged by size:
239 237 226
AvaI C'yCGr_G
Cuts at: 0 25 380 617 702
Size: 25 355 237 85
Fragments arranged by size:
355 237 85 25
BanI G'GyrC_C
Cuts at: 0 331 702
Size: 331 371
BanII G_rGCy'C
Cuts at: 0 239 465 691 702
Size: 239 226 226 11
Fragments arranged by size:
239 226 226 11
BbeI G_GCGC'C
Cuts at: 0 335 702
Size: 335 367
BglI GCCn_nnn'nGGC
Cuts at: 0 288 469 702
Size: 288 181 233
Fragments arranged by size:
288 233 181
BlpI GC'TnA_GC
Cuts at: 0 226 702
Size: 226 476
Bme1580I G_kGCm'C
Cuts at: 0 239 465 702
Size: 239 226 237
Fragments arranged by size:
239 237 226
BmgBI CAC'GTC
Cuts at: 0 80 702
Size: 80 622
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 597 630 702
Size: 597 33 72
Fragments arranged by size:
597 72 33
Bpu10I CC'TnA_GC
Cuts at: 0 4 359 702
Size: 4 355 343
Fragments arranged by size:
355 343 4
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 702
Size: 466 236
BsaI GGTCTCn'nnnn_
Cuts at: 0 268 487 636 702
Size: 268 219 149 66
Fragments arranged by size:
268 219 149 66
BsaHI Gr'CG_yC
Cuts at: 0 332 702
Size: 332 370
BsaWI w'CCGG_w
Cuts at: 0 12 702
Size: 12 690
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 409 439 702
Size: 409 30 263
Fragments arranged by size:
409 263 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 268 543 573 619 702
Size: 268 275 30 46 83
Fragments arranged by size:
275 268 83 46 30
BseYI C'CCAG_C
Cuts at: 0 561 690 702
Size: 561 129 12
Fragments arranged by size:
561 129 12
BsiEI CG_ry'CG
Cuts at: 0 132 702
Size: 132 570
BsiHKAI G_wGCw'C
Cuts at: 0 232 702
Size: 232 470
BsmBI CGTCTCn'nnnn_
Cuts at: 0 256 702
Size: 256 446
Bsp1286I G_dGCh'C
Cuts at: 0 232 239 465 691 702
Size: 232 7 226 226 11
Fragments arranged by size:
232 226 226 11 7
BspEI T'CCGG_A
Cuts at: 0 12 702
Size: 12 690
BsrBI CCG'CTC
Cuts at: 0 75 702
Size: 75 627
BsrFI r'CCGG_y
Cuts at: 0 591 702
Size: 591 111
BssHII G'CGCG_C
Cuts at: 0 113 702
Size: 113 589
BssSI C'ACGA_G
Cuts at: 0 135 702
Size: 135 567
BstXI CCAn_nnnn'nTGG
Cuts at: 0 698 702
Size: 698 4
BtgI C'CryG_G
Cuts at: 0 19 702
Size: 19 683
EaeI y'GGCC_r
Cuts at: 0 129 470 702
Size: 129 341 232
Fragments arranged by size:
341 232 129
EagI C'GGCC_G
Cuts at: 0 129 702
Size: 129 573
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 702
Size: 311 391
EcoNI CCTnn'n_nnAGG
Cuts at: 0 91 601 702
Size: 91 510 101
Fragments arranged by size:
510 101 91
EcoO109I rG'GnC_Cy
Cuts at: 0 236 702
Size: 236 466
HaeII r_GCGC'y
Cuts at: 0 335 378 702
Size: 335 43 324
Fragments arranged by size:
335 324 43
KasI G'GCGC_C
Cuts at: 0 331 702
Size: 331 371
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 290 395 702
Size: 290 105 307
Fragments arranged by size:
307 290 105
MscI TGG'CCA
Cuts at: 0 472 702
Size: 472 230
MslI CAynn'nnrTG
Cuts at: 0 403 702
Size: 403 299
MspA1I CmG'CkG
Cuts at: 0 21 120 225 683 702
Size: 21 99 105 458 19
Fragments arranged by size:
458 105 99 21 19
NaeI GCC'GGC
Cuts at: 0 593 702
Size: 593 109
NarI GG'CG_CC
Cuts at: 0 332 702
Size: 332 370
NgoMIV G'CCGG_C
Cuts at: 0 591 702
Size: 591 111
NotI GC'GGCC_GC
Cuts at: 0 129 702
Size: 129 573
PfoI T'CCnGG_A
Cuts at: 0 92 702
Size: 92 610
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 409 441 702
Size: 409 32 261
Fragments arranged by size:
409 261 32
PspOMI G'GGCC_C
Cuts at: 0 235 461 702
Size: 235 226 241
Fragments arranged by size:
241 235 226
PvuII CAG'CTG
Cuts at: 0 225 683 702
Size: 225 458 19
Fragments arranged by size:
458 225 19
SacII CC_GC'GG
Cuts at: 0 22 702
Size: 22 680
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 469 702
Size: 469 233
SfoI GGC'GCC
Cuts at: 0 333 702
Size: 333 369
SmaI CCC'GGG
Cuts at: 0 27 619 702
Size: 27 592 83
Fragments arranged by size:
592 83 27
SmlI C'TyrA_G
Cuts at: 0 481 702
Size: 481 221
StuI AGG'CCT
Cuts at: 0 610 702
Size: 610 92
StyI C'CwwG_G
Cuts at: 0 340 702
Size: 340 362
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 292 702
Size: 292 410
TatI w'GTAC_w
Cuts at: 0 668 702
Size: 668 34
Tth111I GACn'n_nGTC
Cuts at: 0 63 702
Size: 63 639
XmaI C'CCGG_G
Cuts at: 0 25 617 702
Size: 25 592 85
Fragments arranged by size:
592 85 25
Enzymes that do cut:
AcuI AfeI AleI AloI ApaI AvaI BanI BanII
BbeI BglI BlpI Bme1580I BmgBI BplI Bpu10I BpuEI
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BsrBI BsrFI BssHII BssSI BstXI
BtgI EaeI EagI Eco57MI EcoNI EcoO109I HaeII KasI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NotI
PfoI PpiI PspOMI PvuII SacII SfiI SfoI SmaI
SmlI StuI StyI TaqII TatI Tth111I XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BmrI BmtI BpmI BsaAI BsaBI BsgI
BsiWI BsmI BspHI BspMI BsrDI BsrGI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EarI EciI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HincII HindIII HpaI
KpnI MfeI MluI NcoI NdeI NheI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpuMI
PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TstI
XbaI XcmI XhoI XmnI ZraI