(Linear) (Six-base) MAPSORT of: KIBB8904.seq  Check: 3189  from: 1  to: 711



KIBB8904.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     567     625     711 
   Size:        567      58      86

  Fragments arranged by size: 

                 567      86      58
 
AleI CACnn'nnGTG

Cuts at:      0     367     711 
   Size:        367     344
 
AlwNI CAG_nnn'CTG

Cuts at:      0     600     711 
   Size:        600     111
 
BbvCI CC'TCA_GC

Cuts at:      0     509     711 
   Size:        509     202
 
BlpI GC'TnA_GC

Cuts at:      0     154     711 
   Size:        154     557
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     472     711 
   Size:        472     239
 
Bpu10I CC'TnA_GC

Cuts at:      0     509     711 
   Size:        509     202
 
BsaAI yAC'GTr

Cuts at:      0     442     711 
   Size:        442     269
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     439     469     711 
   Size:        439      30     242

  Fragments arranged by size: 

                 439     242      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      25     711 
   Size:         25     686
 
BseYI C'CCAG_C

Cuts at:      0     354     711 
   Size:        354     357
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     658     711 
   Size:        658      53
 
BsiHKAI G_wGCw'C

Cuts at:      0     374     391     711 
   Size:        374      17     320

  Fragments arranged by size: 

                 374     320      17
 
Bsp1286I G_dGCh'C

Cuts at:      0     374     391     711 
   Size:        374      17     320

  Fragments arranged by size: 

                 374     320      17
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      80     711 
   Size:         80     631
 
BstEII G'GTnAC_C

Cuts at:      0     471     711 
   Size:        471     240
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     468     711 
   Size:        468     243
 
BstYI r'GATC_y

Cuts at:      0      92     711 
   Size:         92     619
 
BtgI C'CryG_G

Cuts at:      0     285     318     711 
   Size:        285      33     393

  Fragments arranged by size: 

                 393     285      33
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     177     711 
   Size:        177     534
 
EarI CTCTTCn'nnn_

Cuts at:      0     541     711 
   Size:        541     170
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     472     567     625     711 
   Size:        472      95      58      86

  Fragments arranged by size: 

                 472      95      86      58
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      76     711 
   Size:         76     635
 
EcoO109I rG'GnC_Cy

Cuts at:      0     654     711 
   Size:        654      57
 
HaeII r_GCGC'y

Cuts at:      0     616     711 
   Size:        616      95
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     439     471     711 
   Size:        439      32     240

  Fragments arranged by size: 

                 439     240      32
 
MslI CAynn'nnrTG

Cuts at:      0     367     711 
   Size:        367     344
 
MspA1I CmG'CkG

Cuts at:      0     287     597     711 
   Size:        287     310     114

  Fragments arranged by size: 

                 310     287     114
 
NspI r_CATG'y

Cuts at:      0     240     711 
   Size:        240     471
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     600     711 
   Size:        600     111
 
PfoI T'CCnGG_A

Cuts at:      0      98     711 
   Size:         98     613
 
PmlI CAC'GTG

Cuts at:      0     442     711 
   Size:        442     269
 
PpuMI rG'GwC_Cy

Cuts at:      0     654     711 
   Size:        654      57
 
PvuII CAG'CTG

Cuts at:      0     597     711 
   Size:        597     114
 
SacII CC_GC'GG

Cuts at:      0     288     711 
   Size:        288     423
 
SfcI C'TryA_G

Cuts at:      0     616     711 
   Size:        616      95
 
StuI AGG'CCT

Cuts at:      0      32     298     711 
   Size:         32     266     413

  Fragments arranged by size: 

                 413     266      32
 
StyI C'CwwG_G

Cuts at:      0      77     711 
   Size:         77     634
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     567     711 
   Size:        567     144
 
TatI w'GTAC_w

Cuts at:      0     110     711 
   Size:        110     601
 

 Enzymes that do cut:

     AcuI     AleI    AlwNI    BbvCI     BlpI     BpmI   Bpu10I    BsaAI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI   BstEII
    BstXI    BstYI     BtgI     DrdI     EarI  Eco57MI    EcoNI EcoO109I
    HaeII    Hin4I     MslI   MspA1I     NspI    PflMI     PfoI     PmlI
    PpuMI    PvuII    SacII     SfcI     StuI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
 Bme1580I    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI    BsaBI
    BsaHI    BsaWI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     EaeI     EagI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI