Restriction map for
pF1KB8905
(Linear) (Six-base) MAPSORT of: KIBB8905.seq Check: 89 from: 1 to: 711
KIBB8905.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 296 499 520 711
Size: 141 155 203 21 191
Fragments arranged by size:
203 191 155 141 21
AlwNI CAG_nnn'CTG
Cuts at: 0 243 316 711
Size: 243 73 395
Fragments arranged by size:
395 243 73
ApaI G_GGCC'C
Cuts at: 0 310 329 711
Size: 310 19 382
Fragments arranged by size:
382 310 19
AvaI C'yCGr_G
Cuts at: 0 626 669 679 711
Size: 626 43 10 32
Fragments arranged by size:
626 43 32 10
BanI G'GyrC_C
Cuts at: 0 190 488 711
Size: 190 298 223
Fragments arranged by size:
298 223 190
BanII G_rGCy'C
Cuts at: 0 121 310 329 352 369 711
Size: 121 189 19 23 17 342
Fragments arranged by size:
342 189 121 23 19 17
BbeI G_GCGC'C
Cuts at: 0 492 711
Size: 492 219
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 405 439 508 542 711
Size: 405 34 69 34 169
Fragments arranged by size:
405 169 69 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 95 580 613 622 711
Size: 95 485 33 9 89
Fragments arranged by size:
485 95 89 33 9
Bme1580I G_kGCm'C
Cuts at: 0 193 310 329 711
Size: 193 117 19 382
Fragments arranged by size:
382 193 117 19
BmgBI CAC'GTC
Cuts at: 0 375 711
Size: 375 336
BmrI ACTGGGnnnn_n'
Cuts at: 0 124 304 711
Size: 124 180 407
Fragments arranged by size:
407 180 124
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 580 711
Size: 226 354 131
Fragments arranged by size:
354 226 131
BsaI GGTCTCn'nnnn_
Cuts at: 0 417 711
Size: 417 294
BsaHI Gr'CG_yC
Cuts at: 0 489 711
Size: 489 222
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 199 229 711
Size: 199 30 482
Fragments arranged by size:
482 199 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 223 370 711
Size: 223 147 341
Fragments arranged by size:
341 223 147
BseYI C'CCAG_C
Cuts at: 0 13 243 250 368 587 711
Size: 13 230 7 118 219 124
Fragments arranged by size:
230 219 124 118 13 7
Bsp1286I G_dGCh'C
Cuts at: 0 121 193 310 329 352 369 711
Size: 121 72 117 19 23 17 342
Fragments arranged by size:
342 121 117 72 23 19 17
BsrBI CCG'CTC
Cuts at: 0 390 674 711
Size: 390 284 37
Fragments arranged by size:
390 284 37
BsrFI r'CCGG_y
Cuts at: 0 437 659 711
Size: 437 222 52
Fragments arranged by size:
437 222 52
BssHII G'CGCG_C
Cuts at: 0 570 711
Size: 570 141
BstXI CCAn_nnnn'nTGG
Cuts at: 0 21 711
Size: 21 690
BtgI C'CryG_G
Cuts at: 0 26 284 330 507 711
Size: 26 258 46 177 204
Fragments arranged by size:
258 204 177 46 26
EaeI y'GGCC_r
Cuts at: 0 23 196 581 711
Size: 23 173 385 130
Fragments arranged by size:
385 173 130 23
EarI CTCTTCn'nnn_
Cuts at: 0 272 709 711
Size: 272 437 2
Fragments arranged by size:
437 272 2
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 226 296 499 520 580 711
Size: 141 85 70 203 21 60 131
Fragments arranged by size:
203 141 131 85 70 60 21
EcoO109I rG'GnC_Cy
Cuts at: 0 306 326 690 711
Size: 306 20 364 21
Fragments arranged by size:
364 306 21 20
HaeII r_GCGC'y
Cuts at: 0 409 472 492 711
Size: 409 63 20 219
Fragments arranged by size:
409 219 63 20
KasI G'GCGC_C
Cuts at: 0 488 711
Size: 488 223
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 422 711
Size: 422 289
MscI TGG'CCA
Cuts at: 0 25 711
Size: 25 686
MspA1I CmG'CkG
Cuts at: 0 243 381 444 509 549 591 711
Size: 243 138 63 65 40 42 120
Fragments arranged by size:
243 138 120 65 63 42 40
NarI GG'CG_CC
Cuts at: 0 489 711
Size: 489 222
NcoI C'CATG_G
Cuts at: 0 26 284 330 711
Size: 26 258 46 381
Fragments arranged by size:
381 258 46 26
PasI CC'CwG_GG
Cuts at: 0 343 711
Size: 343 368
PpuMI rG'GwC_Cy
Cuts at: 0 690 711
Size: 690 21
PspOMI G'GGCC_C
Cuts at: 0 306 325 711
Size: 306 19 386
Fragments arranged by size:
386 306 19
PspXI vC'TCGA_Gb
Cuts at: 0 669 711
Size: 669 42
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 496 528 711
Size: 496 32 183
Fragments arranged by size:
496 183 32
PstI C_TGCA'G
Cuts at: 0 236 299 545 711
Size: 236 63 246 166
Fragments arranged by size:
246 236 166 63
PvuII CAG'CTG
Cuts at: 0 243 381 444 591 711
Size: 243 138 63 147 120
Fragments arranged by size:
243 147 138 120 63
SacII CC_GC'GG
Cuts at: 0 510 711
Size: 510 201
SanDI GG'GwC_CC
Cuts at: 0 690 711
Size: 690 21
SfcI C'TryA_G
Cuts at: 0 104 232 295 541 711
Size: 104 128 63 246 170
Fragments arranged by size:
246 170 128 104 63
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 95 613 622 711
Size: 95 518 9 89
Fragments arranged by size:
518 95 89 9
SfoI GGC'GCC
Cuts at: 0 490 711
Size: 490 221
SmaI CCC'GGG
Cuts at: 0 628 681 711
Size: 628 53 30
Fragments arranged by size:
628 53 30
SmlI C'TyrA_G
Cuts at: 0 669 711
Size: 669 42
StuI AGG'CCT
Cuts at: 0 203 523 711
Size: 203 320 188
Fragments arranged by size:
320 203 188
StyI C'CwwG_G
Cuts at: 0 26 284 330 711
Size: 26 258 46 381
Fragments arranged by size:
381 258 46 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 165 711
Size: 165 546
TatI w'GTAC_w
Cuts at: 0 215 711
Size: 215 496
XhoI C'TCGA_G
Cuts at: 0 669 711
Size: 669 42
XmaI C'CCGG_G
Cuts at: 0 626 679 711
Size: 626 53 32
Fragments arranged by size:
626 53 32
Enzymes that do cut:
AcuI AlwNI ApaI AvaI BanI BanII BbeI BcgI
BglI Bme1580I BmgBI BmrI BpmI BsaI BsaHI BsaXI
BseRI BseYI Bsp1286I BsrBI BsrFI BssHII BstXI BtgI
EaeI EarI Eco57MI EcoO109I HaeII KasI MmeI MscI
MspA1I NarI NcoI PasI PpuMI PspOMI PspXI PsrI
PstI PvuII SacII SanDI SfcI SfiI SfoI SmaI
SmlI StuI StyI TaqII TatI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BciVI
BclI BfrBI BglII BlpI BmtI BplI Bpu10I BpuEI
BsaAI BsaBI BsaWI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrDI BsrGI BssSI BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KpnI MfeI MluI MslI NaeI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PvuI
RsrII SacI SalI SapI SbfI ScaI SexAI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TstI
Tth111I XbaI XcmI XmnI ZraI