Restriction map for
pF1KB8908
(Linear) (Six-base) MAPSORT of: KIBB8908.seq Check: 8738 from: 1 to: 726
KIBB8908.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
ApaI G_GGCC'C
Cuts at: 0 125 726
Size: 125 601
ApoI r'AATT_y
Cuts at: 0 259 726
Size: 259 467
AvaI C'yCGr_G
Cuts at: 0 647 726
Size: 647 79
BanI G'GyrC_C
Cuts at: 0 135 187 726
Size: 135 52 539
Fragments arranged by size:
539 135 52
BanII G_rGCy'C
Cuts at: 0 7 125 306 648 726
Size: 7 118 181 342 78
Fragments arranged by size:
342 181 118 78 7
BbeI G_GCGC'C
Cuts at: 0 139 726
Size: 139 587
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 306 340 726
Size: 306 34 386
Fragments arranged by size:
386 306 34
BglII A'GATC_T
Cuts at: 0 581 726
Size: 581 145
BlpI GC'TnA_GC
Cuts at: 0 235 305 726
Size: 235 70 421
Fragments arranged by size:
421 235 70
Bme1580I G_kGCm'C
Cuts at: 0 125 726
Size: 125 601
BsaI GGTCTCn'nnnn_
Cuts at: 0 420 726
Size: 420 306
BsaHI Gr'CG_yC
Cuts at: 0 136 251 726
Size: 136 115 475
Fragments arranged by size:
475 136 115
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 175 205 435 465 726
Size: 175 30 230 30 261
Fragments arranged by size:
261 230 175 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 670 703 715 726
Size: 670 33 12 11
Fragments arranged by size:
670 33 12 11
BseYI C'CCAG_C
Cuts at: 0 403 726
Size: 403 323
BsiHKAI G_wGCw'C
Cuts at: 0 7 232 726
Size: 7 225 494
Fragments arranged by size:
494 225 7
BsmBI CGTCTCn'nnnn_
Cuts at: 0 242 404 726
Size: 242 162 322
Fragments arranged by size:
322 242 162
Bsp1286I G_dGCh'C
Cuts at: 0 7 125 232 306 648 726
Size: 7 118 107 74 342 78
Fragments arranged by size:
342 118 107 78 74 7
BsrFI r'CCGG_y
Cuts at: 0 43 60 77 470 726
Size: 43 17 17 393 256
Fragments arranged by size:
393 256 43 17 17
BstYI r'GATC_y
Cuts at: 0 581 726
Size: 581 145
BtgI C'CryG_G
Cuts at: 0 183 243 418 726
Size: 183 60 175 308
Fragments arranged by size:
308 183 175 60
BtsI GCAGTG_nn'
Cuts at: 0 456 726
Size: 456 270
EarI CTCTTCn'nnn_
Cuts at: 0 680 692 726
Size: 680 12 34
Fragments arranged by size:
680 34 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 339 726
Size: 339 387
EcoICRI GAG'CTC
Cuts at: 0 5 726
Size: 5 721
HaeII r_GCGC'y
Cuts at: 0 139 280 726
Size: 139 141 446
Fragments arranged by size:
446 141 139
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 328 360 726
Size: 328 32 366
Fragments arranged by size:
366 328 32
HincII GTy'rAC
Cuts at: 0 18 726
Size: 18 708
KasI G'GCGC_C
Cuts at: 0 135 726
Size: 135 591
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 514 726
Size: 227 287 212
Fragments arranged by size:
287 227 212
NaeI GCC'GGC
Cuts at: 0 45 726
Size: 45 681
NarI GG'CG_CC
Cuts at: 0 136 726
Size: 136 590
NcoI C'CATG_G
Cuts at: 0 418 726
Size: 418 308
NgoMIV G'CCGG_C
Cuts at: 0 43 726
Size: 43 683
PasI CC'CwG_GG
Cuts at: 0 555 726
Size: 555 171
PflMI CCAn_nnn'nTGG
Cuts at: 0 111 726
Size: 111 615
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 214 246 726
Size: 214 32 480
Fragments arranged by size:
480 214 32
PspOMI G'GGCC_C
Cuts at: 0 121 726
Size: 121 605
SacI G_AGCT'C
Cuts at: 0 7 726
Size: 7 719
SfoI GGC'GCC
Cuts at: 0 137 726
Size: 137 589
StyI C'CwwG_G
Cuts at: 0 418 482 726
Size: 418 64 244
Fragments arranged by size:
418 244 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 580 726
Size: 580 146
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 175 207 435 467 726
Size: 175 32 228 32 259
Fragments arranged by size:
259 228 175 32 32
Enzymes that do cut:
ApaI ApoI AvaI BanI BanII BbeI BcgI BglII
BlpI Bme1580I BsaI BsaHI BsaXI BseRI BseYI BsiHKAI
BsmBI Bsp1286I BsrFI BstYI BtgI BtsI EarI EciI
EcoICRI HaeII Hin4I HincII KasI MmeI NaeI NarI
NcoI NgoMIV PasI PflMI PpiI PspOMI SacI SfoI
StyI TaqII TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI
BciVI BclI BfrBI BglI BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaAI BsaBI BsaWI BsgI BsiEI
BsiWI BsmI BspEI BspHI BspMI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KpnI MfeI MluI MscI MslI
MspA1I NdeI NheI NotI NruI NsiI NspI PacI
PciI PfoI PmeI PmlI PpuMI PshAI PsiI PspXI
PsrI PstI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI