Restriction map for
pF1KB8909
(Linear) (Six-base) MAPSORT of: KIBB8909.seq Check: 7884 from: 1 to: 732
KIBB8909.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AfeI AGC'GCT
Cuts at: 0 631 732
Size: 631 101
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 589 621 732
Size: 589 32 111
Fragments arranged by size:
589 111 32
AlwNI CAG_nnn'CTG
Cuts at: 0 42 446 732
Size: 42 404 286
Fragments arranged by size:
404 286 42
AvaI C'yCGr_G
Cuts at: 0 147 732
Size: 147 585
BamHI G'GATC_C
Cuts at: 0 280 345 732
Size: 280 65 387
Fragments arranged by size:
387 280 65
BanI G'GyrC_C
Cuts at: 0 69 732
Size: 69 663
BclI T'GATC_A
Cuts at: 0 695 732
Size: 695 37
Bme1580I G_kGCm'C
Cuts at: 0 436 732
Size: 436 296
BmgBI CAC'GTC
Cuts at: 0 294 732
Size: 294 438
BmrI ACTGGGnnnn_n'
Cuts at: 0 30 602 732
Size: 30 572 130
Fragments arranged by size:
572 130 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 407 732
Size: 407 325
Bpu10I CC'TnA_GC
Cuts at: 0 116 732
Size: 116 616
BsaAI yAC'GTr
Cuts at: 0 527 732
Size: 527 205
BsaHI Gr'CG_yC
Cuts at: 0 95 732
Size: 95 637
BsaWI w'CCGG_w
Cuts at: 0 90 581 732
Size: 90 491 151
Fragments arranged by size:
491 151 90
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 349 367 654 732
Size: 349 18 287 78
Fragments arranged by size:
349 287 78 18
BseYI C'CCAG_C
Cuts at: 0 361 688 732
Size: 361 327 44
Fragments arranged by size:
361 327 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 156 264 507 732
Size: 156 108 243 225
Fragments arranged by size:
243 225 156 108
BsiEI CG_ry'CG
Cuts at: 0 11 706 732
Size: 11 695 26
Fragments arranged by size:
695 26 11
BsiHKAI G_wGCw'C
Cuts at: 0 140 732
Size: 140 592
Bsp1286I G_dGCh'C
Cuts at: 0 140 436 732
Size: 140 296 296
Fragments arranged by size:
296 296 140
BspEI T'CCGG_A
Cuts at: 0 90 732
Size: 90 642
BspHI T'CATG_A
Cuts at: 0 635 732
Size: 635 97
BsrBI CCG'CTC
Cuts at: 0 102 369 557 732
Size: 102 267 188 175
Fragments arranged by size:
267 188 175 102
BssHII G'CGCG_C
Cuts at: 0 356 732
Size: 356 376
BstBI TT'CG_AA
Cuts at: 0 55 732
Size: 55 677
BstEII G'GTnAC_C
Cuts at: 0 126 142 180 732
Size: 126 16 38 552
Fragments arranged by size:
552 126 38 16
BstYI r'GATC_y
Cuts at: 0 280 345 732
Size: 280 65 387
Fragments arranged by size:
387 280 65
BtgI C'CryG_G
Cuts at: 0 5 732
Size: 5 727
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 171 732
Size: 171 561
EaeI y'GGCC_r
Cuts at: 0 8 529 719 732
Size: 8 521 190 13
Fragments arranged by size:
521 190 13 8
EagI C'GGCC_G
Cuts at: 0 8 732
Size: 8 724
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 407 732
Size: 407 325
HaeII r_GCGC'y
Cuts at: 0 633 718 732
Size: 633 85 14
Fragments arranged by size:
633 85 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 265 297 330 362 732
Size: 265 32 33 32 370
Fragments arranged by size:
370 265 33 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 308 732
Size: 182 126 424
Fragments arranged by size:
424 182 126
MscI TGG'CCA
Cuts at: 0 531 721 732
Size: 531 190 11
Fragments arranged by size:
531 190 11
MslI CAynn'nnrTG
Cuts at: 0 503 732
Size: 503 229
MspA1I CmG'CkG
Cuts at: 0 7 611 618 732
Size: 7 604 7 114
Fragments arranged by size:
604 114 7 7
NotI GC'GGCC_GC
Cuts at: 0 8 732
Size: 8 724
PflMI CCAn_nnn'nTGG
Cuts at: 0 42 732
Size: 42 690
PfoI T'CCnGG_A
Cuts at: 0 384 732
Size: 384 348
PmlI CAC'GTG
Cuts at: 0 527 732
Size: 527 205
PvuI CG_AT'CG
Cuts at: 0 706 732
Size: 706 26
SacII CC_GC'GG
Cuts at: 0 8 732
Size: 8 724
SfcI C'TryA_G
Cuts at: 0 438 732
Size: 438 294
SmaI CCC'GGG
Cuts at: 0 149 732
Size: 149 583
StyI C'CwwG_G
Cuts at: 0 131 732
Size: 131 601
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 198 732
Size: 198 534
XmaI C'CCGG_G
Cuts at: 0 147 732
Size: 147 585
XmnI GAAnn'nnTTC
Cuts at: 0 631 732
Size: 631 101
Enzymes that do cut:
AfeI AloI AlwNI AvaI BamHI BanI BclI Bme1580I
BmgBI BmrI BpmI Bpu10I BsaAI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BsrBI
BssHII BstBI BstEII BstYI BtgI BtgZI EaeI EagI
Eco57MI HaeII Hin4I MmeI MscI MslI MspA1I NotI
PflMI PfoI PmlI PvuI SacII SfcI SmaI StyI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BanII BbeI BbsI BbvCI
BcgI BciVI BfrBI BglI BglII BlpI BmtI BplI
BpuEI BsaI BsaBI BsaXI BsiWI BsmI BsmBI BspMI
BsrDI BsrFI BsrGI BssSI BstAPI BstXI BstZ17I Bsu36I
BtsI ClaI CspCI DraI DraIII DrdI EarI EciI
EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI
NspI PacI PasI PciI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI TstI Tth111I XbaI XhoI
ZraI