Restriction map for
pF1KB8910
(Linear) (Six-base) MAPSORT of: KIBB8910.seq Check: 9010 from: 1 to: 624
KIBB8910.orf
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AccI GT'mk_AC
Cuts at: 0 236 624
Size: 236 388
AlwNI CAG_nnn'CTG
Cuts at: 0 500 624
Size: 500 124
AvaI C'yCGr_G
Cuts at: 0 82 169 624
Size: 82 87 455
Fragments arranged by size:
455 87 82
BanI G'GyrC_C
Cuts at: 0 212 624
Size: 212 412
BanII G_rGCy'C
Cuts at: 0 68 170 185 492 624
Size: 68 102 15 307 132
Fragments arranged by size:
307 132 102 68 15
BbeI G_GCGC'C
Cuts at: 0 216 624
Size: 216 408
BbsI GAAGACnn'nnnn_
Cuts at: 0 290 624
Size: 290 334
Bme1580I G_kGCm'C
Cuts at: 0 176 624
Size: 176 448
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 169 184 541 624
Size: 127 42 15 357 83
Fragments arranged by size:
357 127 83 42 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 624
Size: 52 572
BsaHI Gr'CG_yC
Cuts at: 0 213 624
Size: 213 411
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 121 151 624
Size: 121 30 473
Fragments arranged by size:
473 121 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7 136 314 624
Size: 7 129 178 310
Fragments arranged by size:
310 178 129 7
BsiEI CG_ry'CG
Cuts at: 0 263 624
Size: 263 361
BsiHKAI G_wGCw'C
Cuts at: 0 68 185 624
Size: 68 117 439
Fragments arranged by size:
439 117 68
Bsp1286I G_dGCh'C
Cuts at: 0 68 170 176 185 492 624
Size: 68 102 6 9 307 132
Fragments arranged by size:
307 132 102 68 9 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 230 624
Size: 230 394
BsrBI CCG'CTC
Cuts at: 0 122 624
Size: 122 502
BstXI CCAn_nnnn'nTGG
Cuts at: 0 147 624
Size: 147 477
DraIII CAC_nnn'GTG
Cuts at: 0 30 483 624
Size: 30 453 141
Fragments arranged by size:
453 141 30
EaeI y'GGCC_r
Cuts at: 0 260 624
Size: 260 364
EagI C'GGCC_G
Cuts at: 0 260 624
Size: 260 364
EarI CTCTTCn'nnn_
Cuts at: 0 139 624
Size: 139 485
EcoICRI GAG'CTC
Cuts at: 0 66 183 624
Size: 66 117 441
Fragments arranged by size:
441 117 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 169 184 541 624
Size: 127 42 15 357 83
Fragments arranged by size:
357 127 83 42 15
EcoO109I rG'GnC_Cy
Cuts at: 0 497 624
Size: 497 127
HaeII r_GCGC'y
Cuts at: 0 216 624
Size: 216 408
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 121 153 624
Size: 121 32 471
Fragments arranged by size:
471 121 32
HincII GTy'rAC
Cuts at: 0 237 624
Size: 237 387
KasI G'GCGC_C
Cuts at: 0 212 624
Size: 212 412
NarI GG'CG_CC
Cuts at: 0 213 624
Size: 213 411
NdeI CA'TA_TG
Cuts at: 0 310 624
Size: 310 314
PflMI CCAn_nnn'nTGG
Cuts at: 0 483 624
Size: 483 141
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 55 87 624
Size: 55 32 537
Fragments arranged by size:
537 55 32
PpuMI rG'GwC_Cy
Cuts at: 0 497 624
Size: 497 127
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 521 553 624
Size: 521 32 71
Fragments arranged by size:
521 71 32
SacI G_AGCT'C
Cuts at: 0 68 185 624
Size: 68 117 439
Fragments arranged by size:
439 117 68
SalI G'TCGA_C
Cuts at: 0 235 624
Size: 235 389
SexAI A'CCwGG_T
Cuts at: 0 479 512 524 624
Size: 479 33 12 100
Fragments arranged by size:
479 100 33 12
SfoI GGC'GCC
Cuts at: 0 214 624
Size: 214 410
SmaI CCC'GGG
Cuts at: 0 171 624
Size: 171 453
SmlI C'TyrA_G
Cuts at: 0 67 624
Size: 67 557
SrfI GCCC'GGGC
Cuts at: 0 171 624
Size: 171 453
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 76 624
Size: 76 548
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 565 624
Size: 565 59
XmaI C'CCGG_G
Cuts at: 0 169 624
Size: 169 455
Enzymes that do cut:
AccI AlwNI AvaI BanI BanII BbeI BbsI Bme1580I
BpmI BpuEI BsaHI BsaXI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrBI BstXI DraIII EaeI EagI EarI EcoICRI
Eco57MI EcoO109I HaeII Hin4I HincII KasI NarI NdeI
PflMI PpiI PpuMI PsrI SacI SalI SexAI SfoI
SmaI SmlI SrfI TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI
BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaWI BseRI
BseYI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacII
SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI
SnaBI SpeI SphI SspI StuI StyI SwaI TatI
TstI Tth111I XbaI XhoI XmnI ZraI