Restriction map for
pF1KB8913
(Linear) (Six-base) MAPSORT of: KIBB8913.seq Check: 8821 from: 1 to: 759
KIBB8913.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AccI GT'mk_AC
Cuts at: 0 593 759
Size: 593 166
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 759
Size: 685 74
AlwNI CAG_nnn'CTG
Cuts at: 0 361 759
Size: 361 398
AvaI C'yCGr_G
Cuts at: 0 74 254 759
Size: 74 180 505
Fragments arranged by size:
505 180 74
BanI G'GyrC_C
Cuts at: 0 124 203 759
Size: 124 79 556
Fragments arranged by size:
556 124 79
BanII G_rGCy'C
Cuts at: 0 44 759
Size: 44 715
BbsI GAAGACnn'nnnn_
Cuts at: 0 473 759
Size: 473 286
BbvCI CC'TCA_GC
Cuts at: 0 56 759
Size: 56 703
BclI T'GATC_A
Cuts at: 0 173 759
Size: 173 586
BglI GCCn_nnn'nGGC
Cuts at: 0 52 759
Size: 52 707
BmrI ACTGGGnnnn_n'
Cuts at: 0 289 759
Size: 289 470
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 613 759
Size: 613 146
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 235 628 759
Size: 127 108 393 131
Fragments arranged by size:
393 131 127 108
Bpu10I CC'TnA_GC
Cuts at: 0 56 759
Size: 56 703
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 759
Size: 117 642
BsaI GGTCTCn'nnnn_
Cuts at: 0 418 759
Size: 418 341
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 664 694 759
Size: 664 30 65
Fragments arranged by size:
664 65 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 253 672 759
Size: 52 201 419 87
Fragments arranged by size:
419 201 87 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 759
Size: 265 494
BsiEI CG_ry'CG
Cuts at: 0 29 759
Size: 29 730
BsiHKAI G_wGCw'C
Cuts at: 0 542 759
Size: 542 217
BsmI GAATG_Cn'
Cuts at: 0 461 759
Size: 461 298
Bsp1286I G_dGCh'C
Cuts at: 0 44 542 759
Size: 44 498 217
Fragments arranged by size:
498 217 44
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 432 759
Size: 432 327
BsrBI CCG'CTC
Cuts at: 0 81 101 759
Size: 81 20 658
Fragments arranged by size:
658 81 20
BsrFI r'CCGG_y
Cuts at: 0 24 200 759
Size: 24 176 559
Fragments arranged by size:
559 176 24
BssHII G'CGCG_C
Cuts at: 0 133 759
Size: 133 626
BtgI C'CryG_G
Cuts at: 0 144 749 759
Size: 144 605 10
Fragments arranged by size:
605 144 10
DrdI GACnn_nn'nnGTC
Cuts at: 0 601 759
Size: 601 158
EaeI y'GGCC_r
Cuts at: 0 8 26 65 147 759
Size: 8 18 39 82 612
Fragments arranged by size:
612 82 39 18 8
EagI C'GGCC_G
Cuts at: 0 26 759
Size: 26 733
EarI CTCTTCn'nnn_
Cuts at: 0 661 759
Size: 661 98
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 252 759
Size: 252 507
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 235 628 685 759
Size: 127 108 393 57 74
Fragments arranged by size:
393 127 108 74 57
EcoNI CCTnn'n_nnAGG
Cuts at: 0 610 759
Size: 610 149
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 201 759
Size: 201 558
HaeII r_GCGC'y
Cuts at: 0 214 262 759
Size: 214 48 497
Fragments arranged by size:
497 214 48
MfeI C'AATT_G
Cuts at: 0 434 759
Size: 434 325
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 443 521 759
Size: 347 96 78 238
Fragments arranged by size:
347 238 96 78
MspA1I CmG'CkG
Cuts at: 0 269 528 759
Size: 269 259 231
Fragments arranged by size:
269 259 231
NaeI GCC'GGC
Cuts at: 0 26 759
Size: 26 733
NcoI C'CATG_G
Cuts at: 0 749 759
Size: 749 10
NgoMIV G'CCGG_C
Cuts at: 0 24 759
Size: 24 735
NspI r_CATG'y
Cuts at: 0 504 759
Size: 504 255
PasI CC'CwG_GG
Cuts at: 0 408 759
Size: 408 351
PfoI T'CCnGG_A
Cuts at: 0 605 759
Size: 605 154
PvuII CAG'CTG
Cuts at: 0 528 759
Size: 528 231
SapI GCTCTTCn'nnn_
Cuts at: 0 661 759
Size: 661 98
SmaI CCC'GGG
Cuts at: 0 76 256 759
Size: 76 180 503
Fragments arranged by size:
503 180 76
SmlI C'TyrA_G
Cuts at: 0 96 759
Size: 96 663
SrfI GCCC'GGGC
Cuts at: 0 256 759
Size: 256 503
StuI AGG'CCT
Cuts at: 0 616 759
Size: 616 143
StyI C'CwwG_G
Cuts at: 0 191 749 759
Size: 191 558 10
Fragments arranged by size:
558 191 10
XmaI C'CCGG_G
Cuts at: 0 74 254 759
Size: 74 180 505
Fragments arranged by size:
505 180 74
XmnI GAAnn'nnTTC
Cuts at: 0 616 659 759
Size: 616 43 100
Fragments arranged by size:
616 100 43
Enzymes that do cut:
AccI AcuI AlwNI AvaI BanI BanII BbsI BbvCI
BclI BglI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaXI BseRI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI
BsrBI BsrFI BssHII BtgI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI FalI HaeII MfeI MmeI MspA1I
NaeI NcoI NgoMIV NspI PasI PfoI PvuII SapI
SmaI SmlI SrfI StuI StyI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI
BfrBI BglII BlpI Bme1580I BmgBI BmtI BsaAI BsaBI
BsaHI BsaWI BseYI BsiWI BsmBI BspEI BspHI BsrDI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII EcoICRI
EcoO109I EcoRI EcoRV FseI FspI FspAI Hin4I HincII
HindIII HpaI KasI KpnI MluI MscI MslI NarI
NdeI NheI NotI NruI NsiI PacI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SspI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI ZraI