Restriction map for
pF1KB8920
(Linear) (Six-base) MAPSORT of: KIBB8920.seq Check: 548 from: 1 to: 801
KIBB8920.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AatII G_ACGT'C
Cuts at: 0 320 801
Size: 320 481
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 801
Size: 37 764
AfeI AGC'GCT
Cuts at: 0 409 706 801
Size: 409 297 95
Fragments arranged by size:
409 297 95
AlwNI CAG_nnn'CTG
Cuts at: 0 207 801
Size: 207 594
ApaI G_GGCC'C
Cuts at: 0 33 394 603 658 801
Size: 33 361 209 55 143
Fragments arranged by size:
361 209 143 55 33
AvaI C'yCGr_G
Cuts at: 0 24 183 361 801
Size: 24 159 178 440
Fragments arranged by size:
440 178 159 24
BanI G'GyrC_C
Cuts at: 0 80 379 461 487 801
Size: 80 299 82 26 314
Fragments arranged by size:
314 299 82 80 26
BanII G_rGCy'C
Cuts at: 0 33 368 394 603 658 801
Size: 33 335 26 209 55 143
Fragments arranged by size:
335 209 143 55 33 26
BbeI G_GCGC'C
Cuts at: 0 465 491 801
Size: 465 26 310
Fragments arranged by size:
465 310 26
BbsI GAAGACnn'nnnn_
Cuts at: 0 338 801
Size: 338 463
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 562 596 801
Size: 562 34 205
Fragments arranged by size:
562 205 34
BglI GCCn_nnn'nGGC
Cuts at: 0 575 801
Size: 575 226
Bme1580I G_kGCm'C
Cuts at: 0 33 394 603 658 801
Size: 33 361 209 55 143
Fragments arranged by size:
361 209 143 55 33
BmrI ACTGGGnnnn_n'
Cuts at: 0 460 801
Size: 460 341
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 336 801
Size: 336 465
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 151 541 634 801
Size: 106 45 390 93 167
Fragments arranged by size:
390 167 106 93 45
BsaHI Gr'CG_yC
Cuts at: 0 317 462 488 742 801
Size: 317 145 26 254 59
Fragments arranged by size:
317 254 145 59 26
BseYI C'CCAG_C
Cuts at: 0 248 434 680 801
Size: 248 186 246 121
Fragments arranged by size:
248 246 186 121
BsiEI CG_ry'CG
Cuts at: 0 167 264 506 638 801
Size: 167 97 242 132 163
Fragments arranged by size:
242 167 163 132 97
BsiHKAI G_wGCw'C
Cuts at: 0 428 620 674 801
Size: 428 192 54 127
Fragments arranged by size:
428 192 127 54
BsmBI CGTCTCn'nnnn_
Cuts at: 0 324 801
Size: 324 477
Bsp1286I G_dGCh'C
Cuts at: 0 33 368 394 428 603 620 658 674
Size: 33 335 26 34 175 17 38 16
Cuts at: 674 801
Size: 127
Fragments arranged by size:
335 175 127 38 34 33 26 17
16
BspHI T'CATG_A
Cuts at: 0 719 801
Size: 719 82
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 134 801
Size: 134 667
BsrBI CCG'CTC
Cuts at: 0 23 801
Size: 23 778
BsrFI r'CCGG_y
Cuts at: 0 444 801
Size: 444 357
BstAPI GCAn_nnn'nTGC
Cuts at: 0 624 801
Size: 624 177
Bsu36I CC'TnA_GG
Cuts at: 0 350 801
Size: 350 451
BtgI C'CryG_G
Cuts at: 0 62 113 419 801
Size: 62 51 306 382
Fragments arranged by size:
382 306 62 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 389 801
Size: 389 412
EaeI y'GGCC_r
Cuts at: 0 164 261 503 543 635 801
Size: 164 97 242 40 92 166
Fragments arranged by size:
242 166 164 97 92 40
EagI C'GGCC_G
Cuts at: 0 164 261 503 635 801
Size: 164 97 242 132 166
Fragments arranged by size:
242 166 164 132 97
EarI CTCTTCn'nnn_
Cuts at: 0 727 801
Size: 727 74
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 106 151 541 634 801
Size: 37 69 45 390 93 167
Fragments arranged by size:
390 167 93 69 45 37
EcoO109I rG'GnC_Cy
Cuts at: 0 29 30 105 599 654 655 801
Size: 29 1 75 494 55 1 146
Fragments arranged by size:
494 146 75 55 29 1 1
HaeII r_GCGC'y
Cuts at: 0 411 465 491 708 801
Size: 411 54 26 217 93
Fragments arranged by size:
411 217 93 54 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 484 516 801
Size: 484 32 285
Fragments arranged by size:
484 285 32
KasI G'GCGC_C
Cuts at: 0 461 487 801
Size: 461 26 314
Fragments arranged by size:
461 314 26
MscI TGG'CCA
Cuts at: 0 545 801
Size: 545 256
MslI CAynn'nnrTG
Cuts at: 0 67 801
Size: 67 734
MspA1I CmG'CkG
Cuts at: 0 248 284 293 325 345 434 680 801
Size: 248 36 9 32 20 89 246 121
Fragments arranged by size:
248 246 121 89 36 32 20 9
NarI GG'CG_CC
Cuts at: 0 462 488 801
Size: 462 26 313
Fragments arranged by size:
462 313 26
NcoI C'CATG_G
Cuts at: 0 62 419 801
Size: 62 357 382
Fragments arranged by size:
382 357 62
PfoI T'CCnGG_A
Cuts at: 0 554 801
Size: 554 247
PpuMI rG'GwC_Cy
Cuts at: 0 105 801
Size: 105 696
PspOMI G'GGCC_C
Cuts at: 0 29 390 599 654 801
Size: 29 361 209 55 147
Fragments arranged by size:
361 209 147 55 29
PstI C_TGCA'G
Cuts at: 0 19 801
Size: 19 782
SapI GCTCTTCn'nnn_
Cuts at: 0 727 801
Size: 727 74
SfcI C'TryA_G
Cuts at: 0 15 660 801
Size: 15 645 141
Fragments arranged by size:
645 141 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 575 801
Size: 575 226
SfoI GGC'GCC
Cuts at: 0 463 489 801
Size: 463 26 312
Fragments arranged by size:
463 312 26
SmaI CCC'GGG
Cuts at: 0 185 801
Size: 185 616
StuI AGG'CCT
Cuts at: 0 58 511 801
Size: 58 453 290
Fragments arranged by size:
453 290 58
StyI C'CwwG_G
Cuts at: 0 62 419 695 801
Size: 62 357 276 106
Fragments arranged by size:
357 276 106 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 226 269 801
Size: 226 43 532
Fragments arranged by size:
532 226 43
XmaI C'CCGG_G
Cuts at: 0 183 801
Size: 183 618
ZraI GAC'GTC
Cuts at: 0 318 801
Size: 318 483
Enzymes that do cut:
AatII AcuI AfeI AlwNI ApaI AvaI BanI BanII
BbeI BbsI BcgI BglI Bme1580I BmrI BplI BpmI
BsaHI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrFI BstAPI Bsu36I BtgI BtgZI EaeI EagI
EarI Eco57MI EcoO109I HaeII Hin4I KasI MscI MslI
MspA1I NarI NcoI PfoI PpuMI PspOMI PstI SapI
SfcI SfiI SfoI SmaI StuI StyI TaqII XmaI
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BglII
BlpI BmgBI BmtI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaWI BsaXI BseRI BsgI BsiWI BsmI BspEI BsrDI
BsrGI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I
BtsI ClaI CspCI DraI DraIII DrdI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI NaeI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TatI TstI Tth111I XbaI
XcmI XhoI XmnI