Restriction map for
pF1KB8922
(Linear) (Six-base) MAPSORT of: KIBB8922.seq Check: 3835 from: 1 to: 807
KIBB8922.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AccI GT'mk_AC
Cuts at: 0 601 807
Size: 601 206
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 807
Size: 237 570
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 619 651 807
Size: 619 32 156
Fragments arranged by size:
619 156 32
AlwNI CAG_nnn'CTG
Cuts at: 0 618 807
Size: 618 189
ApaI G_GGCC'C
Cuts at: 0 591 807
Size: 591 216
AvaI C'yCGr_G
Cuts at: 0 27 289 464 505 807
Size: 27 262 175 41 302
Fragments arranged by size:
302 262 175 41 27
BamHI G'GATC_C
Cuts at: 0 105 807
Size: 105 702
BanI G'GyrC_C
Cuts at: 0 325 807
Size: 325 482
BanII G_rGCy'C
Cuts at: 0 7 217 248 482 591 807
Size: 7 210 31 234 109 216
Fragments arranged by size:
234 216 210 109 31 7
BbeI G_GCGC'C
Cuts at: 0 329 807
Size: 329 478
BbvCI CC'TCA_GC
Cuts at: 0 160 366 807
Size: 160 206 441
Fragments arranged by size:
441 206 160
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 660 694 807
Size: 660 34 113
Fragments arranged by size:
660 113 34
BglI GCCn_nnn'nGGC
Cuts at: 0 510 807
Size: 510 297
BglII A'GATC_T
Cuts at: 0 716 807
Size: 716 91
BlpI GC'TnA_GC
Cuts at: 0 445 807
Size: 445 362
Bme1580I G_kGCm'C
Cuts at: 0 591 807
Size: 591 216
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 360 640 807
Size: 171 189 280 167
Fragments arranged by size:
280 189 171 167
Bpu10I CC'TnA_GC
Cuts at: 0 160 366 807
Size: 160 206 441
Fragments arranged by size:
441 206 160
BsaBI GATnn'nnATC
Cuts at: 0 715 807
Size: 715 92
BsaHI Gr'CG_yC
Cuts at: 0 326 807
Size: 326 481
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 165 174 807
Size: 165 9 633
Fragments arranged by size:
633 165 9
BseYI C'CCAG_C
Cuts at: 0 454 807
Size: 454 353
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 288 807
Size: 288 519
BsiHKAI G_wGCw'C
Cuts at: 0 7 217 807
Size: 7 210 590
Fragments arranged by size:
590 210 7
Bsp1286I G_dGCh'C
Cuts at: 0 7 217 248 482 591 807
Size: 7 210 31 234 109 216
Fragments arranged by size:
234 216 210 109 31 7
BsrBI CCG'CTC
Cuts at: 0 92 173 807
Size: 92 81 634
Fragments arranged by size:
634 92 81
BsrFI r'CCGG_y
Cuts at: 0 124 731 807
Size: 124 607 76
Fragments arranged by size:
607 124 76
BssHII G'CGCG_C
Cuts at: 0 219 807
Size: 219 588
BstYI r'GATC_y
Cuts at: 0 105 716 807
Size: 105 611 91
Fragments arranged by size:
611 105 91
BstZ17I GTA'TAC
Cuts at: 0 602 807
Size: 602 205
Bsu36I CC'TnA_GG
Cuts at: 0 425 807
Size: 425 382
BtgI C'CryG_G
Cuts at: 0 321 482 807
Size: 321 161 325
Fragments arranged by size:
325 321 161
BtsI GCAGTG_nn'
Cuts at: 0 81 807
Size: 81 726
DrdI GACnn_nn'nnGTC
Cuts at: 0 163 807
Size: 163 644
EaeI y'GGCC_r
Cuts at: 0 485 807
Size: 485 322
EcoICRI GAG'CTC
Cuts at: 0 5 215 807
Size: 5 210 592
Fragments arranged by size:
592 210 5
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 237 360 640 807
Size: 171 66 123 280 167
Fragments arranged by size:
280 171 167 123 66
EcoNI CCTnn'n_nnAGG
Cuts at: 0 537 807
Size: 537 270
EcoO109I rG'GnC_Cy
Cuts at: 0 587 807
Size: 587 220
HaeII r_GCGC'y
Cuts at: 0 329 383 708 797 807
Size: 329 54 325 89 10
Fragments arranged by size:
329 325 89 54 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 656 688 807
Size: 656 32 119
Fragments arranged by size:
656 119 32
HincII GTy'rAC
Cuts at: 0 168 807
Size: 168 639
HindIII A'AGCT_T
Cuts at: 0 544 807
Size: 544 263
KasI G'GCGC_C
Cuts at: 0 325 807
Size: 325 482
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 807
Size: 377 430
MscI TGG'CCA
Cuts at: 0 487 807
Size: 487 320
MspA1I CmG'CkG
Cuts at: 0 112 787 807
Size: 112 675 20
Fragments arranged by size:
675 112 20
NarI GG'CG_CC
Cuts at: 0 326 807
Size: 326 481
NcoI C'CATG_G
Cuts at: 0 482 807
Size: 482 325
PflMI CCAn_nnn'nTGG
Cuts at: 0 618 807
Size: 618 189
PspOMI G'GGCC_C
Cuts at: 0 587 807
Size: 587 220
PvuII CAG'CTG
Cuts at: 0 787 807
Size: 787 20
RsrII CG'GwC_CG
Cuts at: 0 593 807
Size: 593 214
SacI G_AGCT'C
Cuts at: 0 7 217 807
Size: 7 210 590
Fragments arranged by size:
590 210 7
SfcI C'TryA_G
Cuts at: 0 782 807
Size: 782 25
SfoI GGC'GCC
Cuts at: 0 327 807
Size: 327 480
StyI C'CwwG_G
Cuts at: 0 482 807
Size: 482 325
Enzymes that do cut:
AccI AcuI AloI AlwNI ApaI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BglI BglII BlpI Bme1580I
BpmI Bpu10I BsaBI BsaHI BseRI BseYI BsgI BsiHKAI
Bsp1286I BsrBI BsrFI BssHII BstYI BstZ17I Bsu36I BtgI
BtsI DrdI EaeI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I HincII HindIII KasI MmeI MscI MspA1I NarI
NcoI PflMI PspOMI PvuII RsrII SacI SfcI SfoI
StyI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbsI BciVI BclI BfrBI BmgBI BmrI
BmtI BplI BpuEI BsaI BsaAI BsaWI BsaXI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI
BssSI BstAPI BstBI BstEII BstXI BtgZI ClaI CspCI
DraI DraIII EagI EarI EciI EcoRI EcoRV FalI
FseI FspI FspAI HpaI KpnI MfeI MluI MslI
NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PstI PvuI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI