(Linear) (Six-base) MAPSORT of: KIBB8928.seq  Check: 8189  from: 1  to: 840



KIBB8928.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:50  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      82     840 
   Size:         82     758
 
AccI GT'mk_AC

Cuts at:      0     197     243     840 
   Size:        197      46     597

  Fragments arranged by size: 

                 597     197      46
 
AclI AA'CG_TT

Cuts at:      0     175     840 
   Size:        175     665
 
AflIII A'CryG_T

Cuts at:      0     134     840 
   Size:        134     706
 
AlwNI CAG_nnn'CTG

Cuts at:      0      24     840 
   Size:         24     816
 
AseI AT'TA_AT

Cuts at:      0     272     840 
   Size:        272     568
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     456     518     840 
   Size:        456      62     322

  Fragments arranged by size: 

                 456     322      62
 
BclI T'GATC_A

Cuts at:      0     522     840 
   Size:        522     318
 
BglII A'GATC_T

Cuts at:      0     439     840 
   Size:        439     401
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     378     840 
   Size:        378     462
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     840 
   Size:        241     599
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     115     840 
   Size:        115     725
 
BsaAI yAC'GTr

Cuts at:      0      81     840 
   Size:         81     759
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     792     840 
   Size:        103     689      48

  Fragments arranged by size: 

                 689     103      48
 
BsiHKAI G_wGCw'C

Cuts at:      0     267     840 
   Size:        267     573
 
Bsp1286I G_dGCh'C

Cuts at:      0     267     840 
   Size:        267     573
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      82     840 
   Size:         82     758
 
BsrBI CCG'CTC

Cuts at:      0      57     840 
   Size:         57     783
 
BsrFI r'CCGG_y

Cuts at:      0      62     840 
   Size:         62     778
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     707     840 
   Size:        707     133
 
BstYI r'GATC_y

Cuts at:      0     378     439     621     840 
   Size:        378      61     182     219

  Fragments arranged by size: 

                 378     219     182      61
 
DraI TTT'AAA

Cuts at:      0     227     840 
   Size:        227     613
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     840 
   Size:        241     599
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     614     840 
   Size:        614     226
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     817     840 
   Size:        817      23
 
HindIII A'AGCT_T

Cuts at:      0     596     840 
   Size:        596     244
 
MfeI C'AATT_G

Cuts at:      0     701     840 
   Size:        701     139
 
MspA1I CmG'CkG

Cuts at:      0     465     840 
   Size:        465     375
 
NaeI GCC'GGC

Cuts at:      0      64     840 
   Size:         64     776
 
NgoMIV G'CCGG_C

Cuts at:      0      62     840 
   Size:         62     778
 
NspI r_CATG'y

Cuts at:      0     126     840 
   Size:        126     714
 
ScaI AGT'ACT

Cuts at:      0     807     840 
   Size:        807      33
 
SfcI C'TryA_G

Cuts at:      0     244     840 
   Size:        244     596
 
SmlI C'TyrA_G

Cuts at:      0      94     840 
   Size:         94     746
 
StyI C'CwwG_G

Cuts at:      0     575     840 
   Size:        575     265
 
TatI w'GTAC_w

Cuts at:      0      26     805     840 
   Size:         26     779      35

  Fragments arranged by size: 

                 779      35      26
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     356     388     840 
   Size:        356      32     452

  Fragments arranged by size: 

                 452     356      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     661     752     840 
   Size:        661      91      88

  Fragments arranged by size: 

                 661      91      88
 
XmnI GAAnn'nnTTC

Cuts at:      0     710     840 
   Size:        710     130
 

 Enzymes that do cut:

     AarI     AccI     AclI   AflIII    AlwNI     AseI     BbsI     BclI
    BglII     BplI     BpmI    BpuEI    BsaAI     BsgI  BsiHKAI Bsp1286I
    BspMI    BsrBI    BsrFI    BstXI    BstYI     DraI  Eco57MI     FalI
    Hin4I  HindIII     MfeI   MspA1I     NaeI   NgoMIV     NspI     ScaI
     SfcI     SmlI     StyI     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AcuI     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     ApoI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI
    BciVI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI
   Bpu10I     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MluI
     MmeI     MscI     MslI     NarI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
  Tth111I     XbaI     XhoI     XmaI     ZraI