Restriction map for
pF1KB8931
(Linear) (Six-base) MAPSORT of: KIBB8931.seq Check: 933 from: 1 to: 846
KIBB8931.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
Acc65I G'GTAC_C
Cuts at: 0 620 846
Size: 620 226
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 846
Size: 340 506
AfeI AGC'GCT
Cuts at: 0 368 846
Size: 368 478
AflIII A'CryG_T
Cuts at: 0 146 846
Size: 146 700
AlwNI CAG_nnn'CTG
Cuts at: 0 495 846
Size: 495 351
ApaI G_GGCC'C
Cuts at: 0 359 846
Size: 359 487
AscI GG'CGCG_CC
Cuts at: 0 611 846
Size: 611 235
AvaI C'yCGr_G
Cuts at: 0 25 109 345 358 846
Size: 25 84 236 13 488
Fragments arranged by size:
488 236 84 25 13
BanI G'GyrC_C
Cuts at: 0 541 620 846
Size: 541 79 226
Fragments arranged by size:
541 226 79
BanII G_rGCy'C
Cuts at: 0 359 599 846
Size: 359 240 247
Fragments arranged by size:
359 247 240
BbeI G_GCGC'C
Cuts at: 0 545 846
Size: 545 301
BglI GCCn_nnn'nGGC
Cuts at: 0 390 846
Size: 390 456
BglII A'GATC_T
Cuts at: 0 776 846
Size: 776 70
Bme1580I G_kGCm'C
Cuts at: 0 359 846
Size: 359 487
BmrI ACTGGGnnnn_n'
Cuts at: 0 208 846
Size: 208 638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 492 706 846
Size: 400 92 214 140
Fragments arranged by size:
400 214 140 92
BsaAI yAC'GTr
Cuts at: 0 245 846
Size: 245 601
BsaHI Gr'CG_yC
Cuts at: 0 542 846
Size: 542 304
BsaWI w'CCGG_w
Cuts at: 0 791 846
Size: 791 55
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 136 166 466 496 679 709 846
Size: 136 30 300 30 183 30 137
Fragments arranged by size:
300 183 137 136 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 343 846
Size: 343 503
BsiEI CG_ry'CG
Cuts at: 0 240 846
Size: 240 606
BsiHKAI G_wGCw'C
Cuts at: 0 154 846
Size: 154 692
Bsp1286I G_dGCh'C
Cuts at: 0 154 359 599 846
Size: 154 205 240 247
Fragments arranged by size:
247 240 205 154
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 759 846
Size: 759 87
BsrBI CCG'CTC
Cuts at: 0 638 846
Size: 638 208
BsrDI GCAATG_nn'
Cuts at: 0 189 846
Size: 189 657
BsrFI r'CCGG_y
Cuts at: 0 544 846
Size: 544 302
BssHII G'CGCG_C
Cuts at: 0 256 611 846
Size: 256 355 235
Fragments arranged by size:
355 256 235
BstBI TT'CG_AA
Cuts at: 0 664 846
Size: 664 182
BstYI r'GATC_y
Cuts at: 0 776 846
Size: 776 70
DrdI GACnn_nn'nnGTC
Cuts at: 0 769 846
Size: 769 77
EaeI y'GGCC_r
Cuts at: 0 163 237 602 846
Size: 163 74 365 244
Fragments arranged by size:
365 244 163 74
EagI C'GGCC_G
Cuts at: 0 237 846
Size: 237 609
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 187 846
Size: 187 659
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 400 492 706 846
Size: 340 60 92 214 140
Fragments arranged by size:
340 214 140 92 60
EcoO109I rG'GnC_Cy
Cuts at: 0 315 463 846
Size: 315 148 383
Fragments arranged by size:
383 315 148
HaeII r_GCGC'y
Cuts at: 0 370 545 729 846
Size: 370 175 184 117
Fragments arranged by size:
370 184 175 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 466 498 846
Size: 466 32 348
Fragments arranged by size:
466 348 32
HincII GTy'rAC
Cuts at: 0 705 846
Size: 705 141
KasI G'GCGC_C
Cuts at: 0 541 846
Size: 541 305
KpnI G_GTAC'C
Cuts at: 0 624 846
Size: 624 222
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 846
Size: 527 319
MspA1I CmG'CkG
Cuts at: 0 42 378 608 846
Size: 42 336 230 238
Fragments arranged by size:
336 238 230 42
NaeI GCC'GGC
Cuts at: 0 546 846
Size: 546 300
NarI GG'CG_CC
Cuts at: 0 542 846
Size: 542 304
NgoMIV G'CCGG_C
Cuts at: 0 544 846
Size: 544 302
PflMI CCAn_nnn'nTGG
Cuts at: 0 495 846
Size: 495 351
PmlI CAC'GTG
Cuts at: 0 245 846
Size: 245 601
PpuMI rG'GwC_Cy
Cuts at: 0 463 846
Size: 463 383
PspOMI G'GGCC_C
Cuts at: 0 355 846
Size: 355 491
SfoI GGC'GCC
Cuts at: 0 543 846
Size: 543 303
SgrAI Cr'CCGG_yG
Cuts at: 0 544 846
Size: 544 302
SmaI CCC'GGG
Cuts at: 0 27 111 347 360 846
Size: 27 84 236 13 486
Fragments arranged by size:
486 236 84 27 13
XmaI C'CCGG_G
Cuts at: 0 25 109 345 358 846
Size: 25 84 236 13 488
Fragments arranged by size:
488 236 84 25 13
Enzymes that do cut:
Acc65I AcuI AfeI AflIII AlwNI ApaI AscI AvaI
BanI BanII BbeI BglI BglII Bme1580I BmrI BpmI
BsaAI BsaHI BsaWI BsaXI BseRI BsiEI BsiHKAI Bsp1286I
BspMI BsrBI BsrDI BsrFI BssHII BstBI BstYI DrdI
EaeI EagI EciI Eco57MI EcoO109I HaeII Hin4I HincII
KasI KpnI MmeI MspA1I NaeI NarI NgoMIV PflMI
PmlI PpuMI PspOMI SfoI SgrAI SmaI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AseI AsiSI AvrII BaeI
BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BlpI
BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaBI BseYI
BsgI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssSI
BstAPI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII EarI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI MfeI
MluI MscI MslI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PfoI PmeI PpiI
PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SgfI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmnI ZraI