Restriction map for
pF1KB8939
(Linear) (Six-base) MAPSORT of: KIBB8939.seq Check: 828 from: 1 to: 873
KIBB8939.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 430 873
Size: 430 443
AlwNI CAG_nnn'CTG
Cuts at: 0 255 386 509 873
Size: 255 131 123 364
Fragments arranged by size:
364 255 131 123
ApaI G_GGCC'C
Cuts at: 0 754 873
Size: 754 119
ApoI r'AATT_y
Cuts at: 0 340 873
Size: 340 533
BanI G'GyrC_C
Cuts at: 0 310 774 873
Size: 310 464 99
Fragments arranged by size:
464 310 99
BanII G_rGCy'C
Cuts at: 0 197 754 871 873
Size: 197 557 117 2
Fragments arranged by size:
557 197 117 2
BbeI G_GCGC'C
Cuts at: 0 314 873
Size: 314 559
BbsI GAAGACnn'nnnn_
Cuts at: 0 652 873
Size: 652 221
BbvCI CC'TCA_GC
Cuts at: 0 697 873
Size: 697 176
BglI GCCn_nnn'nGGC
Cuts at: 0 166 714 873
Size: 166 548 159
Fragments arranged by size:
548 166 159
Bme1580I G_kGCm'C
Cuts at: 0 754 873
Size: 754 119
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 873
Size: 229 644
Bpu10I CC'TnA_GC
Cuts at: 0 697 873
Size: 697 176
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 247 873
Size: 133 114 626
Fragments arranged by size:
626 133 114
BsaI GGTCTCn'nnnn_
Cuts at: 0 370 873
Size: 370 503
BsaHI Gr'CG_yC
Cuts at: 0 311 661 873
Size: 311 350 212
Fragments arranged by size:
350 311 212
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 559 589 853 873
Size: 559 30 264 20
Fragments arranged by size:
559 264 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 388 685 873
Size: 388 297 188
Fragments arranged by size:
388 297 188
BseYI C'CCAG_C
Cuts at: 0 87 754 873
Size: 87 667 119
Fragments arranged by size:
667 119 87
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 873
Size: 712 161
BsiEI CG_ry'CG
Cuts at: 0 300 537 545 873
Size: 300 237 8 328
Fragments arranged by size:
328 300 237 8
BsiHKAI G_wGCw'C
Cuts at: 0 125 149 197 873
Size: 125 24 48 676
Fragments arranged by size:
676 125 48 24
Bsp1286I G_dGCh'C
Cuts at: 0 125 149 197 754 871 873
Size: 125 24 48 557 117 2
Fragments arranged by size:
557 125 117 48 24 2
BsrBI CCG'CTC
Cuts at: 0 84 793 873
Size: 84 709 80
Fragments arranged by size:
709 84 80
BsrFI r'CCGG_y
Cuts at: 0 521 771 873
Size: 521 250 102
Fragments arranged by size:
521 250 102
BssSI C'ACGA_G
Cuts at: 0 856 873
Size: 856 17
BstXI CCAn_nnnn'nTGG
Cuts at: 0 162 858 873
Size: 162 696 15
Fragments arranged by size:
696 162 15
BstYI r'GATC_y
Cuts at: 0 203 873
Size: 203 670
BtgI C'CryG_G
Cuts at: 0 161 177 314 479 813 873
Size: 161 16 137 165 334 60
Fragments arranged by size:
334 165 161 137 60 16
BtsI GCAGTG_nn'
Cuts at: 0 688 767 799 873
Size: 688 79 32 74
Fragments arranged by size:
688 79 74 32
EaeI y'GGCC_r
Cuts at: 0 297 542 873
Size: 297 245 331
Fragments arranged by size:
331 297 245
EagI C'GGCC_G
Cuts at: 0 297 542 873
Size: 297 245 331
Fragments arranged by size:
331 297 245
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 298 487 797 873
Size: 298 189 310 76
Fragments arranged by size:
310 298 189 76
EcoICRI GAG'CTC
Cuts at: 0 195 873
Size: 195 678
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 873
Size: 229 644
EcoO109I rG'GnC_Cy
Cuts at: 0 751 873
Size: 751 122
HaeII r_GCGC'y
Cuts at: 0 314 873
Size: 314 559
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 658 690 853 873
Size: 658 32 163 20
Fragments arranged by size:
658 163 32 20
KasI G'GCGC_C
Cuts at: 0 310 873
Size: 310 563
MslI CAynn'nnrTG
Cuts at: 0 134 812 873
Size: 134 678 61
Fragments arranged by size:
678 134 61
MspA1I CmG'CkG
Cuts at: 0 179 255 348 600 611 873
Size: 179 76 93 252 11 262
Fragments arranged by size:
262 252 179 93 76 11
NarI GG'CG_CC
Cuts at: 0 311 873
Size: 311 562
NcoI C'CATG_G
Cuts at: 0 813 873
Size: 813 60
PasI CC'CwG_GG
Cuts at: 0 306 712 873
Size: 306 406 161
Fragments arranged by size:
406 306 161
PfoI T'CCnGG_A
Cuts at: 0 206 873
Size: 206 667
PspOMI G'GGCC_C
Cuts at: 0 750 873
Size: 750 123
PstI C_TGCA'G
Cuts at: 0 65 251 824 873
Size: 65 186 573 49
Fragments arranged by size:
573 186 65 49
PvuII CAG'CTG
Cuts at: 0 255 873
Size: 255 618
SacI G_AGCT'C
Cuts at: 0 197 873
Size: 197 676
SacII CC_GC'GG
Cuts at: 0 180 873
Size: 180 693
SbfI CC_TGCA'GG
Cuts at: 0 824 873
Size: 824 49
SfcI C'TryA_G
Cuts at: 0 61 247 820 873
Size: 61 186 573 53
Fragments arranged by size:
573 186 61 53
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 166 873
Size: 166 707
SfoI GGC'GCC
Cuts at: 0 312 873
Size: 312 561
SmlI C'TyrA_G
Cuts at: 0 148 262 873
Size: 148 114 611
Fragments arranged by size:
611 148 114
StyI C'CwwG_G
Cuts at: 0 155 551 813 873
Size: 155 396 262 60
Fragments arranged by size:
396 262 155 60
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 661 693 873
Size: 661 32 180
Fragments arranged by size:
661 180 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 162 873
Size: 162 711
Enzymes that do cut:
AlfI AlwNI ApaI ApoI BanI BanII BbeI BbsI
BbvCI BglI Bme1580I BpmI Bpu10I BpuEI BsaI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrBI
BsrFI BssSI BstXI BstYI BtgI BtsI EaeI EagI
EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI MslI
MspA1I NarI NcoI PasI PfoI PspOMI PstI PvuII
SacI SacII SbfI SfcI SfiI SfoI SmlI StyI
TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AloI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI BplI BsaAI
BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrDI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EarI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI NaeI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SalI SanDI SapI ScaI SexAI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI Tth111I XbaI XhoI XmaI
XmnI ZraI