Restriction map for
pF1KB8956
(Linear) (Six-base) MAPSORT of: KIBB8956.seq Check: 2241 from: 1 to: 999
KIBB8956.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 735 982 999
Size: 735 247 17
Fragments arranged by size:
735 247 17
AatII G_ACGT'C
Cuts at: 0 31 999
Size: 31 968
Acc65I G'GTAC_C
Cuts at: 0 226 999
Size: 226 773
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 748 999
Size: 748 251
AfeI AGC'GCT
Cuts at: 0 622 685 999
Size: 622 63 314
Fragments arranged by size:
622 314 63
AflIII A'CryG_T
Cuts at: 0 516 518 999
Size: 516 2 481
Fragments arranged by size:
516 481 2
ApaI G_GGCC'C
Cuts at: 0 701 999
Size: 701 298
ApaLI G'TGCA_C
Cuts at: 0 512 999
Size: 512 487
AvaI C'yCGr_G
Cuts at: 0 106 700 999
Size: 106 594 299
Fragments arranged by size:
594 299 106
BamHI G'GATC_C
Cuts at: 0 361 999
Size: 361 638
BanI G'GyrC_C
Cuts at: 0 13 95 102 193 226 600 819 991
Size: 13 82 7 91 33 374 219 172
Cuts at: 991 999
Size: 8
Fragments arranged by size:
374 219 172 91 82 33 13 8
7
BanII G_rGCy'C
Cuts at: 0 262 701 999
Size: 262 439 298
Fragments arranged by size:
439 298 262
BbeI G_GCGC'C
Cuts at: 0 17 99 106 197 604 823 999
Size: 17 82 7 91 407 219 176
Fragments arranged by size:
407 219 176 91 82 17 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 645 999
Size: 645 354
BglI GCCn_nnn'nGGC
Cuts at: 0 93 266 343 956 999
Size: 93 173 77 613 43
Fragments arranged by size:
613 173 93 77 43
BlpI GC'TnA_GC
Cuts at: 0 289 999
Size: 289 710
Bme1580I G_kGCm'C
Cuts at: 0 115 516 701 999
Size: 115 401 185 298
Fragments arranged by size:
401 298 185 115
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 682 999
Size: 682 317
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 387 999
Size: 387 612
BsaAI yAC'GTr
Cuts at: 0 519 999
Size: 519 480
BsaHI Gr'CG_yC
Cuts at: 0 14 28 96 103 194 214 572 601
Size: 14 14 68 7 91 20 358 29
Cuts at: 601 820 999
Size: 219 179
Fragments arranged by size:
358 219 179 91 68 29 20 14
14 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 265 295 999
Size: 265 30 704
Fragments arranged by size:
704 265 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 79 310 430 595 598 601 604 999
Size: 79 231 120 165 3 3 3 395
Fragments arranged by size:
395 231 165 120 79 3 3 3
BseYI C'CCAG_C
Cuts at: 0 269 959 999
Size: 269 690 40
Fragments arranged by size:
690 269 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 552 999
Size: 552 447
BsiEI CG_ry'CG
Cuts at: 0 471 999
Size: 471 528
BsiHKAI G_wGCw'C
Cuts at: 0 516 999
Size: 516 483
BsmBI CGTCTCn'nnnn_
Cuts at: 0 221 465 733 999
Size: 221 244 268 266
Fragments arranged by size:
268 266 244 221
Bsp1286I G_dGCh'C
Cuts at: 0 115 262 516 701 999
Size: 115 147 254 185 298
Fragments arranged by size:
298 254 185 147 115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 639 735 815 887 982 999
Size: 639 96 80 72 95 17
Fragments arranged by size:
639 96 95 80 72 17
BsrFI r'CCGG_y
Cuts at: 0 223 346 381 541 564 594 788 999
Size: 223 123 35 160 23 30 194 211
Fragments arranged by size:
223 211 194 160 123 35 30 23
BssHII G'CGCG_C
Cuts at: 0 705 999
Size: 705 294
BstXI CCAn_nnnn'nTGG
Cuts at: 0 837 999
Size: 837 162
BstYI r'GATC_y
Cuts at: 0 361 999
Size: 361 638
BtgI C'CryG_G
Cuts at: 0 131 425 999
Size: 131 294 574
Fragments arranged by size:
574 294 131
BtsI GCAGTG_nn'
Cuts at: 0 916 999
Size: 916 83
EaeI y'GGCC_r
Cuts at: 0 80 128 375 395 716 839 898 999
Size: 80 48 247 20 321 123 59 101
Fragments arranged by size:
321 247 123 101 80 59 48 20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 441 999
Size: 441 558
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 682 748 999
Size: 682 66 251
Fragments arranged by size:
682 251 66
EcoNI CCTnn'n_nnAGG
Cuts at: 0 415 999
Size: 415 584
FseI GG_CCGG'CC
Cuts at: 0 350 999
Size: 350 649
FspI TGC'GCA
Cuts at: 0 978 999
Size: 978 21
HaeII r_GCGC'y
Cuts at: 0 17 99 106 197 318 604 624 669
Size: 17 82 7 91 121 286 20 45
Cuts at: 669 687 789 823 967 999
Size: 18 102 34 144 32
Fragments arranged by size:
286 144 121 102 91 82 45 34
32 20 18 17 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 265 297 999
Size: 265 32 702
Fragments arranged by size:
702 265 32
KasI G'GCGC_C
Cuts at: 0 13 95 102 193 600 819 999
Size: 13 82 7 91 407 219 180
Fragments arranged by size:
407 219 180 91 82 13 7
KpnI G_GTAC'C
Cuts at: 0 230 999
Size: 230 769
MspA1I CmG'CkG
Cuts at: 0 118 133 142 238 341 427 536 560
Size: 118 15 9 96 103 86 109 24
Cuts at: 560 982 999
Size: 422 17
Fragments arranged by size:
422 118 109 103 96 86 24 17
15 9
NaeI GCC'GGC
Cuts at: 0 348 543 566 596 790 999
Size: 348 195 23 30 194 209
Fragments arranged by size:
348 209 195 194 30 23
NarI GG'CG_CC
Cuts at: 0 14 96 103 194 601 820 999
Size: 14 82 7 91 407 219 179
Fragments arranged by size:
407 219 179 91 82 14 7
NgoMIV G'CCGG_C
Cuts at: 0 346 541 564 594 788 999
Size: 346 195 23 30 194 211
Fragments arranged by size:
346 211 195 194 30 23
PmlI CAC'GTG
Cuts at: 0 519 999
Size: 519 480
PspOMI G'GGCC_C
Cuts at: 0 697 999
Size: 697 302
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 757 789 999
Size: 757 32 210
Fragments arranged by size:
757 210 32
PstI C_TGCA'G
Cuts at: 0 923 999
Size: 923 76
PvuII CAG'CTG
Cuts at: 0 238 982 999
Size: 238 744 17
Fragments arranged by size:
744 238 17
SacII CC_GC'GG
Cuts at: 0 134 428 999
Size: 134 294 571
Fragments arranged by size:
571 294 134
SfcI C'TryA_G
Cuts at: 0 919 999
Size: 919 80
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 343 999
Size: 343 656
SfoI GGC'GCC
Cuts at: 0 15 97 104 195 602 821 999
Size: 15 82 7 91 407 219 178
Fragments arranged by size:
407 219 178 91 82 15 7
SmaI CCC'GGG
Cuts at: 0 108 999
Size: 108 891
SmlI C'TyrA_G
Cuts at: 0 366 999
Size: 366 633
StyI C'CwwG_G
Cuts at: 0 478 999
Size: 478 521
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 499 999
Size: 499 500
XmaI C'CCGG_G
Cuts at: 0 106 999
Size: 106 893
ZraI GAC'GTC
Cuts at: 0 29 999
Size: 29 970
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AflIII ApaI ApaLI
AvaI BamHI BanI BanII BbeI BbsI BglI BlpI
Bme1580I BpmI BpuEI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BssHII
BstXI BstYI BtgI BtsI EaeI EciI Eco57MI EcoNI
FseI FspI HaeII Hin4I KasI KpnI MspA1I NaeI
NarI NgoMIV PmlI PspOMI PsrI PstI PvuII SacII
SfcI SfiI SfoI SmaI SmlI StyI TaqII XmaI
ZraI
Enzymes that do not cut:
AccI AclI AflII AgeI AhdI AleI AlfI AloI
AlwNI ApoI AscI AseI AsiSI AvrII BaeI BbvCI
BcgI BciVI BclI BfrBI BglII BmgBI BmrI BmtI
BplI Bpu10I BsaI BsaBI BsaWI BsiWI BsmI BspEI
BspHI BsrBI BsrDI BsrGI BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EarI EcoICRI EcoO109I EcoRI EcoRV FalI FspAI
HincII HindIII HpaI MfeI MluI MmeI MscI MslI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspXI PvuI RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI TstI Tth111I XbaI
XcmI XhoI XmnI