Restriction map for
pF1KB8962
(Linear) (Six-base) MAPSORT of: KIBB8962.seq Check: 3529 from: 1 to: 1026
KIBB8962.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
Acc65I G'GTAC_C
Cuts at: 0 426 1026
Size: 426 600
AfeI AGC'GCT
Cuts at: 0 70 1026
Size: 70 956
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 112 1026
Size: 112 914
AlwNI CAG_nnn'CTG
Cuts at: 0 657 840 1026
Size: 657 183 186
Fragments arranged by size:
657 186 183
ApaI G_GGCC'C
Cuts at: 0 667 1026
Size: 667 359
ApoI r'AATT_y
Cuts at: 0 856 1026
Size: 856 170
AvaI C'yCGr_G
Cuts at: 0 370 456 1026
Size: 370 86 570
Fragments arranged by size:
570 370 86
BamHI G'GATC_C
Cuts at: 0 992 1026
Size: 992 34
BanI G'GyrC_C
Cuts at: 0 426 815 1026
Size: 426 389 211
Fragments arranged by size:
426 389 211
BanII G_rGCy'C
Cuts at: 0 44 146 667 679 1026
Size: 44 102 521 12 347
Fragments arranged by size:
521 347 102 44 12
BbvCI CC'TCA_GC
Cuts at: 0 569 1026
Size: 569 457
BglI GCCn_nnn'nGGC
Cuts at: 0 662 1026
Size: 662 364
Bme1580I G_kGCm'C
Cuts at: 0 437 667 820 1026
Size: 437 230 153 206
Fragments arranged by size:
437 230 206 153
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 702 916 1026
Size: 702 214 110
Fragments arranged by size:
702 214 110
Bpu10I CC'TnA_GC
Cuts at: 0 569 1026
Size: 569 457
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 952 1026
Size: 952 74
BsaI GGTCTCn'nnnn_
Cuts at: 0 592 1026
Size: 592 434
BsaHI Gr'CG_yC
Cuts at: 0 481 1026
Size: 481 545
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 77 334 766 1026
Size: 77 257 432 260
Fragments arranged by size:
432 260 257 77
BseYI C'CCAG_C
Cuts at: 0 184 436 573 625 1026
Size: 184 252 137 52 401
Fragments arranged by size:
401 252 184 137 52
BsiHKAI G_wGCw'C
Cuts at: 0 422 1026
Size: 422 604
BsiWI C'GTAC_G
Cuts at: 0 32 1026
Size: 32 994
Bsp1286I G_dGCh'C
Cuts at: 0 44 146 422 437 667 679 820 1026
Size: 44 102 276 15 230 12 141 206
Fragments arranged by size:
276 230 206 141 102 44 15 12
BsrBI CCG'CTC
Cuts at: 0 800 1026
Size: 800 226
BsrDI GCAATG_nn'
Cuts at: 0 21 1026
Size: 21 1005
BsrGI T'GTAC_A
Cuts at: 0 338 1026
Size: 338 688
BssHII G'CGCG_C
Cuts at: 0 546 1026
Size: 546 480
BssSI C'ACGA_G
Cuts at: 0 421 1026
Size: 421 605
BstBI TT'CG_AA
Cuts at: 0 509 1026
Size: 509 517
BstXI CCAn_nnnn'nTGG
Cuts at: 0 493 1026
Size: 493 533
BstYI r'GATC_y
Cuts at: 0 992 1026
Size: 992 34
DraIII CAC_nnn'GTG
Cuts at: 0 310 1026
Size: 310 716
DrdI GACnn_nn'nnGTC
Cuts at: 0 606 931 1026
Size: 606 325 95
Fragments arranged by size:
606 325 95
EarI CTCTTCn'nnn_
Cuts at: 0 805 1026
Size: 805 221
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 74 1026
Size: 74 952
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 702 916 1026
Size: 702 214 110
Fragments arranged by size:
702 214 110
EcoO109I rG'GnC_Cy
Cuts at: 0 664 1026
Size: 664 362
HaeII r_GCGC'y
Cuts at: 0 72 891 1026
Size: 72 819 135
Fragments arranged by size:
819 135 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 1026
Size: 26 1000
KpnI G_GTAC'C
Cuts at: 0 430 1026
Size: 430 596
MfeI C'AATT_G
Cuts at: 0 117 1026
Size: 117 909
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 635 1026
Size: 635 391
MslI CAynn'nnrTG
Cuts at: 0 94 1026
Size: 94 932
MspA1I CmG'CkG
Cuts at: 0 225 285 528 573 1026
Size: 225 60 243 45 453
Fragments arranged by size:
453 243 225 60 45
NspI r_CATG'y
Cuts at: 0 8 93 339 1026
Size: 8 85 246 687
Fragments arranged by size:
687 246 85 8
PflMI CCAn_nnn'nTGG
Cuts at: 0 840 1026
Size: 840 186
PfoI T'CCnGG_A
Cuts at: 0 995 1026
Size: 995 31
PspOMI G'GGCC_C
Cuts at: 0 663 1026
Size: 663 363
PvuII CAG'CTG
Cuts at: 0 225 573 1026
Size: 225 348 453
Fragments arranged by size:
453 348 225
SfcI C'TryA_G
Cuts at: 0 72 1026
Size: 72 954
SmlI C'TyrA_G
Cuts at: 0 967 1026
Size: 967 59
StyI C'CwwG_G
Cuts at: 0 44 411 1026
Size: 44 367 615
Fragments arranged by size:
615 367 44
TatI w'GTAC_w
Cuts at: 0 338 1026
Size: 338 688
Enzymes that do cut:
Acc65I AfeI AlfI AlwNI ApaI ApoI AvaI BamHI
BanI BanII BbvCI BglI Bme1580I BpmI Bpu10I BpuEI
BsaI BsaHI BseRI BseYI BsiHKAI BsiWI Bsp1286I BsrBI
BsrDI BsrGI BssHII BssSI BstBI BstXI BstYI DraIII
DrdI EarI EciI Eco57MI EcoO109I HaeII Hin4I KpnI
MfeI MmeI MslI MspA1I NspI PflMI PfoI PspOMI
PvuII SfcI SmlI StyI TatI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AflII AflIII AgeI
AhdI AleI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmrI BmtI BplI BsaAI BsaBI BsaWI
BsaXI BsgI BsiEI BsmI BsmBI BspEI BspHI BspMI
BsrFI BstAPI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI EaeI EagI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI MluI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI