Restriction map for
pF1KB8964
(Linear) (Six-base) MAPSORT of: KIBB8964.seq Check: 1791 from: 1 to: 1050
KIBB8964.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 216 1050
Size: 216 834
AccI GT'mk_AC
Cuts at: 0 71 1007 1050
Size: 71 936 43
Fragments arranged by size:
936 71 43
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 895 1007 1050
Size: 895 112 43
Fragments arranged by size:
895 112 43
AfeI AGC'GCT
Cuts at: 0 478 1050
Size: 478 572
AflIII A'CryG_T
Cuts at: 0 596 1050
Size: 596 454
AlwNI CAG_nnn'CTG
Cuts at: 0 208 706 772 1050
Size: 208 498 66 278
Fragments arranged by size:
498 278 208 66
ApaI G_GGCC'C
Cuts at: 0 227 282 576 1050
Size: 227 55 294 474
Fragments arranged by size:
474 294 227 55
ApaLI G'TGCA_C
Cuts at: 0 298 1050
Size: 298 752
AvaI C'yCGr_G
Cuts at: 0 282 1050
Size: 282 768
BanI G'GyrC_C
Cuts at: 0 195 396 1050
Size: 195 201 654
Fragments arranged by size:
654 201 195
BanII G_rGCy'C
Cuts at: 0 10 164 227 282 565 576 810 863
Size: 10 154 63 55 283 11 234 53
Cuts at: 863 1050
Size: 187
Fragments arranged by size:
283 234 187 154 63 55 53 11
10
BbeI G_GCGC'C
Cuts at: 0 400 1050
Size: 400 650
BfrBI ATG'CAT
Cuts at: 0 1045 1050
Size: 1045 5
Bme1580I G_kGCm'C
Cuts at: 0 95 227 282 302 576 1050
Size: 95 132 55 20 274 474
Fragments arranged by size:
474 274 132 95 55 20
BmgBI CAC'GTC
Cuts at: 0 963 1050
Size: 963 87
BmrI ACTGGGnnnn_n'
Cuts at: 0 82 579 1050
Size: 82 497 471
Fragments arranged by size:
497 471 82
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 90 1050
Size: 90 960
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 749 877 1022 1050
Size: 664 85 128 145 28
Fragments arranged by size:
664 145 128 85 28
Bpu10I CC'TnA_GC
Cuts at: 0 588 773 1050
Size: 588 185 277
Fragments arranged by size:
588 277 185
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 438 1050
Size: 438 612
BsaHI Gr'CG_yC
Cuts at: 0 397 1050
Size: 397 653
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 895 925 1050
Size: 895 30 125
Fragments arranged by size:
895 125 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 661 1050
Size: 661 389
BseYI C'CCAG_C
Cuts at: 0 3 201 583 752 1050
Size: 3 198 382 169 298
Fragments arranged by size:
382 298 198 169 3
BsiHKAI G_wGCw'C
Cuts at: 0 302 863 1050
Size: 302 561 187
Fragments arranged by size:
561 302 187
Bsp1286I G_dGCh'C
Cuts at: 0 10 95 164 227 282 302 565 576
Size: 10 85 69 63 55 20 263 11
Cuts at: 576 810 863 1050
Size: 234 53 187
Fragments arranged by size:
263 234 187 85 69 63 55 53
20 11 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 216 626 1050
Size: 216 410 424
Fragments arranged by size:
424 410 216
BsrBI CCG'CTC
Cuts at: 0 1031 1050
Size: 1031 19
BsrFI r'CCGG_y
Cuts at: 0 78 1050
Size: 78 972
BstEII G'GTnAC_C
Cuts at: 0 81 1050
Size: 81 969
Bsu36I CC'TnA_GG
Cuts at: 0 183 779 1050
Size: 183 596 271
Fragments arranged by size:
596 271 183
BtgI C'CryG_G
Cuts at: 0 98 380 728 1050
Size: 98 282 348 322
Fragments arranged by size:
348 322 282 98
BtsI GCAGTG_nn'
Cuts at: 0 175 1050
Size: 175 875
DrdI GACnn_nn'nnGTC
Cuts at: 0 523 1050
Size: 523 527
EaeI y'GGCC_r
Cuts at: 0 35 383 731 1050
Size: 35 348 348 319
Fragments arranged by size:
348 348 319 35
EarI CTCTTCn'nnn_
Cuts at: 0 454 1050
Size: 454 596
EcoICRI GAG'CTC
Cuts at: 0 861 1050
Size: 861 189
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 749 877 895 1007 1022 1050
Size: 664 85 128 18 112 15 28
Fragments arranged by size:
664 128 112 85 28 18 15
EcoO109I rG'GnC_Cy
Cuts at: 0 187 223 278 279 286 573 1017 1050
Size: 187 36 55 1 7 287 444 33
Fragments arranged by size:
444 287 187 55 36 33 7 1
HaeII r_GCGC'y
Cuts at: 0 400 480 1050
Size: 400 80 570
Fragments arranged by size:
570 400 80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 114 146 805 837 1050
Size: 114 32 659 32 213
Fragments arranged by size:
659 213 114 32 32
KasI G'GCGC_C
Cuts at: 0 396 1050
Size: 396 654
MscI TGG'CCA
Cuts at: 0 385 1050
Size: 385 665
MslI CAynn'nnrTG
Cuts at: 0 379 1050
Size: 379 671
MspA1I CmG'CkG
Cuts at: 0 251 671 730 1050
Size: 251 420 59 320
Fragments arranged by size:
420 320 251 59
NarI GG'CG_CC
Cuts at: 0 397 1050
Size: 397 653
NcoI C'CATG_G
Cuts at: 0 98 380 1050
Size: 98 282 670
Fragments arranged by size:
670 282 98
NsiI A_TGCA'T
Cuts at: 0 1047 1050
Size: 1047 3
NspI r_CATG'y
Cuts at: 0 600 1050
Size: 600 450
PasI CC'CwG_GG
Cuts at: 0 154 219 786 865 1050
Size: 154 65 567 79 185
Fragments arranged by size:
567 185 154 79 65
PciI A'CATG_T
Cuts at: 0 596 1050
Size: 596 454
PflMI CCAn_nnn'nTGG
Cuts at: 0 33 1050
Size: 33 1017
PpuMI rG'GwC_Cy
Cuts at: 0 187 286 1050
Size: 187 99 764
Fragments arranged by size:
764 187 99
PspOMI G'GGCC_C
Cuts at: 0 223 278 572 1050
Size: 223 55 294 478
Fragments arranged by size:
478 294 223 55
PstI C_TGCA'G
Cuts at: 0 213 1050
Size: 213 837
SacI G_AGCT'C
Cuts at: 0 863 1050
Size: 863 187
SacII CC_GC'GG
Cuts at: 0 731 1050
Size: 731 319
SfcI C'TryA_G
Cuts at: 0 209 1050
Size: 209 841
SfoI GGC'GCC
Cuts at: 0 398 1050
Size: 398 652
SgrAI Cr'CCGG_yG
Cuts at: 0 78 1050
Size: 78 972
SmaI CCC'GGG
Cuts at: 0 284 1050
Size: 284 766
SmlI C'TyrA_G
Cuts at: 0 417 1050
Size: 417 633
StuI AGG'CCT
Cuts at: 0 239 1050
Size: 239 811
StyI C'CwwG_G
Cuts at: 0 98 380 1050
Size: 98 282 670
Fragments arranged by size:
670 282 98
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 232 1050
Size: 232 818
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 895 927 1050
Size: 895 32 123
Fragments arranged by size:
895 123 32
Tth111I GACn'n_nGTC
Cuts at: 0 702 1050
Size: 702 348
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 960 1050
Size: 960 90
XmaI C'CCGG_G
Cuts at: 0 282 1050
Size: 282 768
XmnI GAAnn'nnTTC
Cuts at: 0 634 1050
Size: 634 416
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AlwNI ApaI ApaLI
AvaI BanI BanII BbeI BfrBI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BseYI
BsiHKAI Bsp1286I BspMI BsrBI BsrFI BstEII Bsu36I BtgI
BtsI DrdI EaeI EarI EcoICRI Eco57MI EcoO109I HaeII
Hin4I KasI MscI MslI MspA1I NarI NcoI NsiI
NspI PasI PciI PflMI PpuMI PspOMI PstI SacI
SacII SfcI SfoI SgrAI SmaI SmlI StuI StyI
TaqII TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AleI AlfI
AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
BmtI BsaI BsaAI BsaBI BsaWI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstXI BstYI BstZ17I BtgZI ClaI CspCI
DraI DraIII EagI EciI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MmeI NaeI NdeI NgoMIV NheI NotI NruI
PacI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SnaBI SpeI SphI SrfI
SspI SwaI TatI XbaI XhoI ZraI