Restriction map for
pF1KB8969
(Linear) (Six-base) MAPSORT of: KIBB8969.seq Check: 5018 from: 1 to: 1077
KIBB8969.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AflII C'TTAA_G
Cuts at: 0 934 1077
Size: 934 143
AflIII A'CryG_T
Cuts at: 0 450 1077
Size: 450 627
AlwNI CAG_nnn'CTG
Cuts at: 0 753 1077
Size: 753 324
ApoI r'AATT_y
Cuts at: 0 533 691 1017 1077
Size: 533 158 326 60
Fragments arranged by size:
533 326 158 60
BamHI G'GATC_C
Cuts at: 0 293 1077
Size: 293 784
BanII G_rGCy'C
Cuts at: 0 629 790 1077
Size: 629 161 287
Fragments arranged by size:
629 287 161
BbsI GAAGACnn'nnnn_
Cuts at: 0 578 1077
Size: 578 499
BglII A'GATC_T
Cuts at: 0 721 1077
Size: 721 356
Bme1580I G_kGCm'C
Cuts at: 0 364 1077
Size: 364 713
BmrI ACTGGGnnnn_n'
Cuts at: 0 594 1077
Size: 594 483
BsaHI Gr'CG_yC
Cuts at: 0 541 1077
Size: 541 536
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 962 992 1077
Size: 962 30 85
Fragments arranged by size:
962 85 30
Bsp1286I G_dGCh'C
Cuts at: 0 364 629 790 1077
Size: 364 265 161 287
Fragments arranged by size:
364 287 265 161
BspHI T'CATG_A
Cuts at: 0 107 152 1077
Size: 107 45 925
Fragments arranged by size:
925 107 45
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 70 1077
Size: 70 1007
BsrBI CCG'CTC
Cuts at: 0 396 1077
Size: 396 681
BsrDI GCAATG_nn'
Cuts at: 0 663 1077
Size: 663 414
BsrFI r'CCGG_y
Cuts at: 0 77 1077
Size: 77 1000
BstXI CCAn_nnnn'nTGG
Cuts at: 0 216 1077
Size: 216 861
BstYI r'GATC_y
Cuts at: 0 293 721 897 1077
Size: 293 428 176 180
Fragments arranged by size:
428 293 180 176
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 126 1077
Size: 126 951
BtsI GCAGTG_nn'
Cuts at: 0 384 1077
Size: 384 693
DraI TTT'AAA
Cuts at: 0 696 1077
Size: 696 381
EaeI y'GGCC_r
Cuts at: 0 206 237 386 806 1077
Size: 206 31 149 420 271
Fragments arranged by size:
420 271 206 149 31
EcoO109I rG'GnC_Cy
Cuts at: 0 548 1077
Size: 548 529
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 962 994 1077
Size: 962 32 83
Fragments arranged by size:
962 83 32
HindIII A'AGCT_T
Cuts at: 0 949 1077
Size: 949 128
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 763 1077
Size: 763 314
MscI TGG'CCA
Cuts at: 0 208 239 388 808 1077
Size: 208 31 149 420 269
Fragments arranged by size:
420 269 208 149 31
MslI CAynn'nnrTG
Cuts at: 0 112 151 651 1077
Size: 112 39 500 426
Fragments arranged by size:
500 426 112 39
MspA1I CmG'CkG
Cuts at: 0 40 1077
Size: 40 1037
NspI r_CATG'y
Cuts at: 0 454 979 1077
Size: 454 525 98
Fragments arranged by size:
525 454 98
PciI A'CATG_T
Cuts at: 0 450 1077
Size: 450 627
PflMI CCAn_nnn'nTGG
Cuts at: 0 753 1077
Size: 753 324
PpuMI rG'GwC_Cy
Cuts at: 0 548 1077
Size: 548 529
PshAI GACnn'nnGTC
Cuts at: 0 180 1077
Size: 180 897
PstI C_TGCA'G
Cuts at: 0 39 1077
Size: 39 1038
PvuII CAG'CTG
Cuts at: 0 40 1077
Size: 40 1037
SexAI A'CCwGG_T
Cuts at: 0 550 1077
Size: 550 527
SfcI C'TryA_G
Cuts at: 0 35 1077
Size: 35 1042
SmlI C'TyrA_G
Cuts at: 0 934 1077
Size: 934 143
SspI AAT'ATT
Cuts at: 0 422 1077
Size: 422 655
StyI C'CwwG_G
Cuts at: 0 185 513 636 901 1077
Size: 185 328 123 265 176
Fragments arranged by size:
328 265 185 176 123
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 247 1077
Size: 247 830
XmnI GAAnn'nnTTC
Cuts at: 0 831 1077
Size: 831 246
Enzymes that do cut:
AflII AflIII AlwNI ApoI BamHI BanII BbsI BglII
Bme1580I BmrI BsaHI BsaXI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BstXI BstYI BtgZI BtsI DraI EaeI
EcoO109I Hin4I HindIII MmeI MscI MslI MspA1I NspI
PciI PflMI PpuMI PshAI PstI PvuII SexAI SfcI
SmlI SspI StyI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BanI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BlpI BmgBI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgI ClaI CspCI DraIII DrdI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HpaI KasI KpnI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PfoI PmeI PmlI PpiI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI TaqII
TatI TstI Tth111I XbaI XhoI XmaI ZraI