Restriction map for
pF1KB8979
(Linear) (Six-base) MAPSORT of: KIBB8979.seq Check: 6161 from: 1 to: 1122
KIBB8979.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 739 1122
Size: 739 383
Acc65I G'GTAC_C
Cuts at: 0 257 1122
Size: 257 865
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 233 345 712 1122
Size: 233 112 367 410
Fragments arranged by size:
410 367 233 112
AflII C'TTAA_G
Cuts at: 0 273 1122
Size: 273 849
AflIII A'CryG_T
Cuts at: 0 527 637 1122
Size: 527 110 485
Fragments arranged by size:
527 485 110
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 641 1122
Size: 641 481
AlwNI CAG_nnn'CTG
Cuts at: 0 25 1122
Size: 25 1097
ApoI r'AATT_y
Cuts at: 0 135 1122
Size: 135 987
BanI G'GyrC_C
Cuts at: 0 257 1122
Size: 257 865
BanII G_rGCy'C
Cuts at: 0 129 1122
Size: 129 993
BfrBI ATG'CAT
Cuts at: 0 871 1122
Size: 871 251
Bme1580I G_kGCm'C
Cuts at: 0 211 1122
Size: 211 911
BmrI ACTGGGnnnn_n'
Cuts at: 0 483 1122
Size: 483 639
BsaWI w'CCGG_w
Cuts at: 0 857 1122
Size: 857 265
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 775 805 1122
Size: 775 30 317
Fragments arranged by size:
775 317 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 901 1122
Size: 901 221
BsiHKAI G_wGCw'C
Cuts at: 0 129 1122
Size: 129 993
BsmI GAATG_Cn'
Cuts at: 0 799 1122
Size: 799 323
Bsp1286I G_dGCh'C
Cuts at: 0 129 211 1122
Size: 129 82 911
Fragments arranged by size:
911 129 82
BspEI T'CCGG_A
Cuts at: 0 857 1122
Size: 857 265
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 300 739 1122
Size: 300 439 383
Fragments arranged by size:
439 383 300
BsrDI GCAATG_nn'
Cuts at: 0 407 433 1122
Size: 407 26 689
Fragments arranged by size:
689 407 26
BstYI r'GATC_y
Cuts at: 0 370 1059 1122
Size: 370 689 63
Fragments arranged by size:
689 370 63
BtgI C'CryG_G
Cuts at: 0 251 1122
Size: 251 871
BtsI GCAGTG_nn'
Cuts at: 0 1103 1122
Size: 1103 19
EaeI y'GGCC_r
Cuts at: 0 329 404 578 1122
Size: 329 75 174 544
Fragments arranged by size:
544 329 174 75
EarI CTCTTCn'nnn_
Cuts at: 0 140 1122
Size: 140 982
EcoICRI GAG'CTC
Cuts at: 0 127 1122
Size: 127 995
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 233 345 712 1122
Size: 233 112 367 410
Fragments arranged by size:
410 367 233 112
EcoO109I rG'GnC_Cy
Cuts at: 0 489 606 1122
Size: 489 117 516
Fragments arranged by size:
516 489 117
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 117 1122
Size: 117 1005
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 438 470 748 780 1122
Size: 438 32 278 32 342
Fragments arranged by size:
438 342 278 32 32
KpnI G_GTAC'C
Cuts at: 0 261 1122
Size: 261 861
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 798 1122
Size: 798 324
MscI TGG'CCA
Cuts at: 0 406 580 1122
Size: 406 174 542
Fragments arranged by size:
542 406 174
MslI CAynn'nnrTG
Cuts at: 0 233 461 623 919 1122
Size: 233 228 162 296 203
Fragments arranged by size:
296 233 228 203 162
MspA1I CmG'CkG
Cuts at: 0 22 1122
Size: 22 1100
NcoI C'CATG_G
Cuts at: 0 251 1122
Size: 251 871
NsiI A_TGCA'T
Cuts at: 0 873 1122
Size: 873 249
NspI r_CATG'y
Cuts at: 0 531 641 1122
Size: 531 110 481
Fragments arranged by size:
531 481 110
PciI A'CATG_T
Cuts at: 0 527 637 1122
Size: 527 110 485
Fragments arranged by size:
527 485 110
PpuMI rG'GwC_Cy
Cuts at: 0 489 1122
Size: 489 633
PvuII CAG'CTG
Cuts at: 0 22 1122
Size: 22 1100
SacI G_AGCT'C
Cuts at: 0 129 1122
Size: 129 993
ScaI AGT'ACT
Cuts at: 0 279 1122
Size: 279 843
SmlI C'TyrA_G
Cuts at: 0 273 1122
Size: 273 849
SspI AAT'ATT
Cuts at: 0 703 954 1122
Size: 703 251 168
Fragments arranged by size:
703 251 168
StyI C'CwwG_G
Cuts at: 0 164 251 629 794 1122
Size: 164 87 378 165 328
Fragments arranged by size:
378 328 165 164 87
TatI w'GTAC_w
Cuts at: 0 277 530 1122
Size: 277 253 592
Fragments arranged by size:
592 277 253
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 303 1122
Size: 303 819
Enzymes that do cut:
AarI Acc65I AcuI AflII AflIII AlfI AlwNI ApoI
BanI BanII BfrBI Bme1580I BmrI BsaWI BsaXI BseRI
BsiHKAI BsmI Bsp1286I BspEI BspMI BsrDI BstYI BtgI
BtsI EaeI EarI EcoICRI Eco57MI EcoO109I FalI Hin4I
KpnI MmeI MscI MslI MspA1I NcoI NsiI NspI
PciI PpuMI PvuII SacI ScaI SmlI SspI StyI
TatI XcmI
Enzymes that do not cut:
AatII AccI AclI AfeI AgeI AhdI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI BmgBI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BseYI BsgI BsiEI BsiWI
BsmBI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PasI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacII SalI SanDI SapI SbfI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI SwaI TaqII TstI Tth111I XbaI XhoI XmaI
XmnI ZraI