Restriction map for
pF1KB8984
(Linear) (Six-base) MAPSORT of: KIBB8984.seq Check: 8004 from: 1 to: 1158
KIBB8984.orf
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1056 1158
Size: 1056 102
AfeI AGC'GCT
Cuts at: 0 349 1158
Size: 349 809
AleI CACnn'nnGTG
Cuts at: 0 28 1158
Size: 28 1130
AlwNI CAG_nnn'CTG
Cuts at: 0 629 1158
Size: 629 529
ApaI G_GGCC'C
Cuts at: 0 398 531 1005 1158
Size: 398 133 474 153
Fragments arranged by size:
474 398 153 133
AscI GG'CGCG_CC
Cuts at: 0 130 1158
Size: 130 1028
AvaI C'yCGr_G
Cuts at: 0 254 934 1158
Size: 254 680 224
Fragments arranged by size:
680 254 224
BanI G'GyrC_C
Cuts at: 0 187 541 1158
Size: 187 354 617
Fragments arranged by size:
617 354 187
BanII G_rGCy'C
Cuts at: 0 40 205 398 531 667 1005 1076 1158
Size: 40 165 193 133 136 338 71 82
Fragments arranged by size:
338 193 165 136 133 82 71 40
BbeI G_GCGC'C
Cuts at: 0 545 1158
Size: 545 613
BbsI GAAGACnn'nnnn_
Cuts at: 0 864 888 1158
Size: 864 24 270
Fragments arranged by size:
864 270 24
BbvCI CC'TCA_GC
Cuts at: 0 622 1158
Size: 622 536
BglI GCCn_nnn'nGGC
Cuts at: 0 412 662 1158
Size: 412 250 496
Fragments arranged by size:
496 412 250
BlpI GC'TnA_GC
Cuts at: 0 361 1158
Size: 361 797
Bme1580I G_kGCm'C
Cuts at: 0 190 398 531 1005 1158
Size: 190 208 133 474 153
Fragments arranged by size:
474 208 190 153 133
Bpu10I CC'TnA_GC
Cuts at: 0 112 199 622 1158
Size: 112 87 423 536
Fragments arranged by size:
536 423 112 87
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 892 1158
Size: 892 266
BsaAI yAC'GTr
Cuts at: 0 30 1158
Size: 30 1128
BsaHI Gr'CG_yC
Cuts at: 0 313 542 1158
Size: 313 229 616
Fragments arranged by size:
616 313 229
BsaWI w'CCGG_w
Cuts at: 0 160 688 1158
Size: 160 528 470
Fragments arranged by size:
528 470 160
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1002 1158
Size: 1002 156
BseYI C'CCAG_C
Cuts at: 0 183 243 465 524 530 971 1158
Size: 183 60 222 59 6 441 187
Fragments arranged by size:
441 222 187 183 60 59 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 1158
Size: 33 1125
BsiEI CG_ry'CG
Cuts at: 0 127 209 682 1158
Size: 127 82 473 476
Fragments arranged by size:
476 473 127 82
BsiHKAI G_wGCw'C
Cuts at: 0 754 1158
Size: 754 404
Bsp1286I G_dGCh'C
Cuts at: 0 40 190 205 398 531 667 754 1005
Size: 40 150 15 193 133 136 87 251
Cuts at: 1005 1076 1158
Size: 71 82
Fragments arranged by size:
251 193 150 136 133 87 82 71
40 15
BspHI T'CATG_A
Cuts at: 0 728 1158
Size: 728 430
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 188 1056 1158
Size: 188 868 102
Fragments arranged by size:
868 188 102
BsrFI r'CCGG_y
Cuts at: 0 48 76 126 565 1158
Size: 48 28 50 439 593
Fragments arranged by size:
593 439 50 48 28
BssHII G'CGCG_C
Cuts at: 0 130 226 452 1158
Size: 130 96 226 706
Fragments arranged by size:
706 226 130 96
BstAPI GCAn_nnn'nTGC
Cuts at: 0 506 1158
Size: 506 652
BstEII G'GTnAC_C
Cuts at: 0 156 615 1158
Size: 156 459 543
Fragments arranged by size:
543 459 156
BstXI CCAn_nnnn'nTGG
Cuts at: 0 342 1158
Size: 342 816
BtgI C'CryG_G
Cuts at: 0 335 341 676 945 1109 1158
Size: 335 6 335 269 164 49
Fragments arranged by size:
335 335 269 164 49 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 290 1158
Size: 290 868
BtsI GCAGTG_nn'
Cuts at: 0 813 1158
Size: 813 345
DrdI GACnn_nn'nnGTC
Cuts at: 0 868 1158
Size: 868 290
EaeI y'GGCC_r
Cuts at: 0 50 74 170 206 338 679 743 1158
Size: 50 24 96 36 132 341 64 415
Fragments arranged by size:
415 341 132 96 64 50 36 24
EagI C'GGCC_G
Cuts at: 0 206 679 1158
Size: 206 473 479
Fragments arranged by size:
479 473 206
EarI CTCTTCn'nnn_
Cuts at: 0 451 1158
Size: 451 707
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1158
Size: 19 1139
EcoNI CCTnn'n_nnAGG
Cuts at: 0 984 1158
Size: 984 174
EcoO109I rG'GnC_Cy
Cuts at: 0 395 819 993 1001 1002 1158
Size: 395 424 174 8 1 156
Fragments arranged by size:
424 395 174 156 8 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 912 1158
Size: 912 246
FseI GG_CCGG'CC
Cuts at: 0 52 1158
Size: 52 1106
HaeII r_GCGC'y
Cuts at: 0 351 361 418 545 657 1109 1158
Size: 351 10 57 127 112 452 49
Fragments arranged by size:
452 351 127 112 57 49 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 896 928 1158
Size: 896 32 230
Fragments arranged by size:
896 230 32
KasI G'GCGC_C
Cuts at: 0 541 1158
Size: 541 617
MslI CAynn'nnrTG
Cuts at: 0 28 64 340 733 745 1158
Size: 28 36 276 393 12 413
Fragments arranged by size:
413 393 276 36 28 12
MspA1I CmG'CkG
Cuts at: 0 387 401 503 773 1100 1158
Size: 387 14 102 270 327 58
Fragments arranged by size:
387 327 270 102 58 14
NaeI GCC'GGC
Cuts at: 0 50 567 1158
Size: 50 517 591
Fragments arranged by size:
591 517 50
NarI GG'CG_CC
Cuts at: 0 542 1158
Size: 542 616
NcoI C'CATG_G
Cuts at: 0 335 1158
Size: 335 823
NgoMIV G'CCGG_C
Cuts at: 0 48 565 1158
Size: 48 517 593
Fragments arranged by size:
593 517 48
NspI r_CATG'y
Cuts at: 0 522 639 1158
Size: 522 117 519
Fragments arranged by size:
522 519 117
PasI CC'CwG_GG
Cuts at: 0 102 866 1158
Size: 102 764 292
Fragments arranged by size:
764 292 102
PfoI T'CCnGG_A
Cuts at: 0 857 982 1158
Size: 857 125 176
Fragments arranged by size:
857 176 125
PpuMI rG'GwC_Cy
Cuts at: 0 819 993 1158
Size: 819 174 165
Fragments arranged by size:
819 174 165
PshAI GACnn'nnGTC
Cuts at: 0 736 1158
Size: 736 422
PspOMI G'GGCC_C
Cuts at: 0 394 527 1001 1158
Size: 394 133 474 157
Fragments arranged by size:
474 394 157 133
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 10 42 1158
Size: 10 32 1116
Fragments arranged by size:
1116 32 10
PvuII CAG'CTG
Cuts at: 0 387 503 1158
Size: 387 116 655
Fragments arranged by size:
655 387 116
SanDI GG'GwC_CC
Cuts at: 0 993 1158
Size: 993 165
ScaI AGT'ACT
Cuts at: 0 490 1158
Size: 490 668
SfoI GGC'GCC
Cuts at: 0 543 1158
Size: 543 615
SmlI C'TyrA_G
Cuts at: 0 907 1158
Size: 907 251
SphI G_CATG'C
Cuts at: 0 522 1158
Size: 522 636
StyI C'CwwG_G
Cuts at: 0 335 997 1158
Size: 335 662 161
Fragments arranged by size:
662 335 161
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 603 796 1158
Size: 603 193 362
Fragments arranged by size:
603 362 193
TatI w'GTAC_w
Cuts at: 0 488 1158
Size: 488 670
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 180 1158
Size: 180 978
Enzymes that do cut:
AarI AfeI AleI AlwNI ApaI AscI AvaI BanI
BanII BbeI BbsI BbvCI BglI BlpI Bme1580I Bpu10I
BpuEI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrFI BssHII BstAPI BstEII
BstXI BtgI BtgZI BtsI DrdI EaeI EagI EarI
EciI EcoNI EcoO109I FalI FseI HaeII Hin4I KasI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PfoI PpuMI PshAI PspOMI PsrI PvuII SanDI ScaI
SfoI SmlI SphI StyI TaqII TatI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlfI AloI ApaLI ApoI AseI AsiSI AvrII
BaeI BamHI BcgI BciVI BclI BfrBI BglII BmgBI
BmrI BmtI BplI BpmI BsaI BsaBI BsaXI BsiWI
BsmI BsmBI BspEI BsrBI BsrDI BsrGI BssSI BstBI
BstYI BstZ17I Bsu36I ClaI CspCI DraI DraIII EcoICRI
Eco57MI EcoRI EcoRV FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI NdeI NheI NotI
NruI NsiI PacI PciI PflMI PmeI PmlI PpiI
PsiI PspXI PstI PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfcI SfiI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI StuI SwaI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI