Restriction map for
pF1KB8986
(Linear) (Six-base) MAPSORT of: KIBB8986.seq Check: 5728 from: 1 to: 1161
KIBB8986.orf
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
Acc65I G'GTAC_C
Cuts at: 0 476 1161
Size: 476 685
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 714 1161
Size: 714 447
AflII C'TTAA_G
Cuts at: 0 862 1161
Size: 862 299
AleI CACnn'nnGTG
Cuts at: 0 1133 1161
Size: 1133 28
AlwNI CAG_nnn'CTG
Cuts at: 0 747 1161
Size: 747 414
ApoI r'AATT_y
Cuts at: 0 240 403 681 1161
Size: 240 163 278 480
Fragments arranged by size:
480 278 240 163
AvaI C'yCGr_G
Cuts at: 0 634 1161
Size: 634 527
AvrII C'CTAG_G
Cuts at: 0 546 1161
Size: 546 615
BanI G'GyrC_C
Cuts at: 0 476 1161
Size: 476 685
BanII G_rGCy'C
Cuts at: 0 55 1161
Size: 55 1106
BbvCI CC'TCA_GC
Cuts at: 0 149 1161
Size: 149 1012
BfrBI ATG'CAT
Cuts at: 0 976 1147 1161
Size: 976 171 14
Fragments arranged by size:
976 171 14
BglI GCCn_nnn'nGGC
Cuts at: 0 85 1161
Size: 85 1076
BmrI ACTGGGnnnn_n'
Cuts at: 0 579 878 1161
Size: 579 299 283
Fragments arranged by size:
579 299 283
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 628 874 1161
Size: 34 594 246 287
Fragments arranged by size:
594 287 246 34
Bpu10I CC'TnA_GC
Cuts at: 0 149 1161
Size: 149 1012
BsaHI Gr'CG_yC
Cuts at: 0 533 551 1161
Size: 533 18 610
Fragments arranged by size:
610 533 18
BsaWI w'CCGG_w
Cuts at: 0 515 1161
Size: 515 646
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1059 1161
Size: 1059 102
BseYI C'CCAG_C
Cuts at: 0 982 1161
Size: 982 179
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 544 1161
Size: 544 617
BsiHKAI G_wGCw'C
Cuts at: 0 55 665 1161
Size: 55 610 496
Fragments arranged by size:
610 496 55
BsmI GAATG_Cn'
Cuts at: 0 622 1161
Size: 622 539
BsmBI CGTCTCn'nnnn_
Cuts at: 0 850 1161
Size: 850 311
Bsp1286I G_dGCh'C
Cuts at: 0 55 665 1161
Size: 55 610 496
Fragments arranged by size:
610 496 55
BsrDI GCAATG_nn'
Cuts at: 0 675 1147 1161
Size: 675 472 14
Fragments arranged by size:
675 472 14
BssSI C'ACGA_G
Cuts at: 0 1131 1161
Size: 1131 30
BstXI CCAn_nnnn'nTGG
Cuts at: 0 231 492 573 1018 1161
Size: 231 261 81 445 143
Fragments arranged by size:
445 261 231 143 81
BstYI r'GATC_y
Cuts at: 0 903 1161
Size: 903 258
BtgI C'CryG_G
Cuts at: 0 95 1161
Size: 95 1066
DrdI GACnn_nn'nnGTC
Cuts at: 0 175 1161
Size: 175 986
EaeI y'GGCC_r
Cuts at: 0 77 482 494 1161
Size: 77 405 12 667
Fragments arranged by size:
667 405 77 12
EarI CTCTTCn'nnn_
Cuts at: 0 43 826 1161
Size: 43 783 335
Fragments arranged by size:
783 335 43
EcoICRI GAG'CTC
Cuts at: 0 53 1161
Size: 53 1108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 628 714 874 1161
Size: 34 594 86 160 287
Fragments arranged by size:
594 287 160 86 34
EcoO109I rG'GnC_Cy
Cuts at: 0 86 381 639 1161
Size: 86 295 258 522
Fragments arranged by size:
522 295 258 86
HaeII r_GCGC'y
Cuts at: 0 1050 1161
Size: 1050 111
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 888 920 1054 1086 1161
Size: 888 32 134 32 75
Fragments arranged by size:
888 134 75 32 32
KpnI G_GTAC'C
Cuts at: 0 480 1161
Size: 480 681
MscI TGG'CCA
Cuts at: 0 79 484 1161
Size: 79 405 677
Fragments arranged by size:
677 405 79
MslI CAynn'nnrTG
Cuts at: 0 571 1016 1100 1133 1161
Size: 571 445 84 33 28
Fragments arranged by size:
571 445 84 33 28
NcoI C'CATG_G
Cuts at: 0 95 1161
Size: 95 1066
NsiI A_TGCA'T
Cuts at: 0 978 1149 1161
Size: 978 171 12
Fragments arranged by size:
978 171 12
PfoI T'CCnGG_A
Cuts at: 0 644 851 1161
Size: 644 207 310
Fragments arranged by size:
644 310 207
PpuMI rG'GwC_Cy
Cuts at: 0 639 1161
Size: 639 522
RsrII CG'GwC_CG
Cuts at: 0 518 1161
Size: 518 643
SacI G_AGCT'C
Cuts at: 0 55 1161
Size: 55 1106
SapI GCTCTTCn'nnn_
Cuts at: 0 43 1161
Size: 43 1118
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 85 1161
Size: 85 1076
SmlI C'TyrA_G
Cuts at: 0 862 1161
Size: 862 299
StuI AGG'CCT
Cuts at: 0 154 1161
Size: 154 1007
StyI C'CwwG_G
Cuts at: 0 95 546 621 1161
Size: 95 451 75 540
Fragments arranged by size:
540 451 95 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 56 1161
Size: 56 1105
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 231 1135 1161
Size: 231 904 26
Fragments arranged by size:
904 231 26
Enzymes that do cut:
Acc65I AcuI AflII AleI AlwNI ApoI AvaI AvrII
BanI BanII BbvCI BfrBI BglI BmrI BpmI Bpu10I
BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BsrDI BssSI BstXI BstYI BtgI DrdI EaeI
EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I KpnI MscI
MslI NcoI NsiI PfoI PpuMI RsrII SacI SapI
SfiI SmlI StuI StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflIII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI BaeI
BamHI BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmtI BplI BpuEI BsaI BsaAI BsaBI
BsaXI BsiEI BsiWI BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII EagI EciI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI MfeI MluI MmeI MspA1I NaeI NarI
NdeI NgoMIV NheI NotI NruI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII SacII SalI SanDI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI