Restriction map for
pF1KB8988
(Linear) (Six-base) MAPSORT of: KIBB8988.seq Check: 1139 from: 1 to: 1164
KIBB8988.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 716 1164
Size: 716 448
AflIII A'CryG_T
Cuts at: 0 584 1164
Size: 584 580
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 526 1164
Size: 526 638
ApoI r'AATT_y
Cuts at: 0 991 1164
Size: 991 173
AvaI C'yCGr_G
Cuts at: 0 31 40 105 390 416 570 1164
Size: 31 9 65 285 26 154 594
Fragments arranged by size:
594 285 154 65 31 26 9
BanI G'GyrC_C
Cuts at: 0 565 716 1164
Size: 565 151 448
Fragments arranged by size:
565 448 151
BanII G_rGCy'C
Cuts at: 0 121 224 409 775 1164
Size: 121 103 185 366 389
Fragments arranged by size:
389 366 185 121 103
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 975 1009 1164
Size: 975 34 155
Fragments arranged by size:
975 155 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 142 1164
Size: 142 1022
BglI GCCn_nnn'nGGC
Cuts at: 0 524 1039 1164
Size: 524 515 125
Fragments arranged by size:
524 515 125
BlpI GC'TnA_GC
Cuts at: 0 223 1164
Size: 223 941
Bme1580I G_kGCm'C
Cuts at: 0 49 719 1164
Size: 49 670 445
Fragments arranged by size:
670 445 49
BmgBI CAC'GTC
Cuts at: 0 633 1164
Size: 633 531
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 658 1164
Size: 658 506
Bpu10I CC'TnA_GC
Cuts at: 0 920 1164
Size: 920 244
BsaI GGTCTCn'nnnn_
Cuts at: 0 43 1074 1093 1164
Size: 43 1031 19 71
Fragments arranged by size:
1031 71 43 19
BsaAI yAC'GTr
Cuts at: 0 282 587 1139 1164
Size: 282 305 552 25
Fragments arranged by size:
552 305 282 25
BsaHI Gr'CG_yC
Cuts at: 0 59 800 938 1164
Size: 59 741 138 226
Fragments arranged by size:
741 226 138 59
BsaWI w'CCGG_w
Cuts at: 0 729 1065 1164
Size: 729 336 99
Fragments arranged by size:
729 336 99
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 103 133 1012 1042 1164
Size: 103 30 879 30 122
Fragments arranged by size:
879 122 103 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 651 691 1164
Size: 651 40 473
Fragments arranged by size:
651 473 40
BseYI C'CCAG_C
Cuts at: 0 12 234 465 514 1164
Size: 12 222 231 49 650
Fragments arranged by size:
650 231 222 49 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 214 1164
Size: 193 21 950
Fragments arranged by size:
950 193 21
BsiEI CG_ry'CG
Cuts at: 0 92 346 1164
Size: 92 254 818
Fragments arranged by size:
818 254 92
BsiHKAI G_wGCw'C
Cuts at: 0 224 409 926 1164
Size: 224 185 517 238
Fragments arranged by size:
517 238 224 185
BsiWI C'GTAC_G
Cuts at: 0 278 593 1164
Size: 278 315 571
Fragments arranged by size:
571 315 278
BsmBI CGTCTCn'nnnn_
Cuts at: 0 807 1164
Size: 807 357
Bsp1286I G_dGCh'C
Cuts at: 0 49 121 224 409 719 775 926 1164
Size: 49 72 103 185 310 56 151 238
Fragments arranged by size:
310 238 185 151 103 72 56 49
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 215 716 1164
Size: 215 501 448
Fragments arranged by size:
501 448 215
BsrBI CCG'CTC
Cuts at: 0 297 1164
Size: 297 867
BsrDI GCAATG_nn'
Cuts at: 0 993 1164
Size: 993 171
BsrFI r'CCGG_y
Cuts at: 0 87 341 543 1164
Size: 87 254 202 621
Fragments arranged by size:
621 254 202 87
BssSI C'ACGA_G
Cuts at: 0 535 1164
Size: 535 629
BstAPI GCAn_nnn'nTGC
Cuts at: 0 315 1164
Size: 315 849
BtgI C'CryG_G
Cuts at: 0 1043 1130 1164
Size: 1043 87 34
Fragments arranged by size:
1043 87 34
BtsI GCAGTG_nn'
Cuts at: 0 197 503 582 1164
Size: 197 306 79 582
Fragments arranged by size:
582 306 197 79
EaeI y'GGCC_r
Cuts at: 0 89 343 684 786 857 1017 1133 1164
Size: 89 254 341 102 71 160 116 31
Fragments arranged by size:
341 254 160 116 102 89 71 31
EagI C'GGCC_G
Cuts at: 0 89 343 1164
Size: 89 254 821
Fragments arranged by size:
821 254 89
EarI CTCTTCn'nnn_
Cuts at: 0 265 436 1164
Size: 265 171 728
Fragments arranged by size:
728 265 171
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 307 1020 1164
Size: 307 713 144
Fragments arranged by size:
713 307 144
EcoICRI GAG'CTC
Cuts at: 0 222 407 1164
Size: 222 185 757
Fragments arranged by size:
757 222 185
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 658 1164
Size: 658 506
EcoO109I rG'GnC_Cy
Cuts at: 0 1090 1164
Size: 1090 74
EcoRI G'AATT_C
Cuts at: 0 991 1164
Size: 991 173
HaeII r_GCGC'y
Cuts at: 0 256 466 1164
Size: 256 210 698
Fragments arranged by size:
698 256 210
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 64 96 103 135 1164
Size: 64 32 7 32 1029
Fragments arranged by size:
1029 64 32 32 7
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 905 1164
Size: 50 855 259
Fragments arranged by size:
855 259 50
MscI TGG'CCA
Cuts at: 0 686 788 1019 1164
Size: 686 102 231 145
Fragments arranged by size:
686 231 145 102
NaeI GCC'GGC
Cuts at: 0 89 545 1164
Size: 89 456 619
Fragments arranged by size:
619 456 89
NcoI C'CATG_G
Cuts at: 0 1043 1164
Size: 1043 121
NgoMIV G'CCGG_C
Cuts at: 0 87 543 1164
Size: 87 456 621
Fragments arranged by size:
621 456 87
NspI r_CATG'y
Cuts at: 0 318 1063 1164
Size: 318 745 101
Fragments arranged by size:
745 318 101
PasI CC'CwG_GG
Cuts at: 0 768 1164
Size: 768 396
PflMI CCAn_nnn'nTGG
Cuts at: 0 768 1164
Size: 768 396
PpuMI rG'GwC_Cy
Cuts at: 0 1090 1164
Size: 1090 74
PstI C_TGCA'G
Cuts at: 0 311 1164
Size: 311 853
SacI G_AGCT'C
Cuts at: 0 224 409 1164
Size: 224 185 755
Fragments arranged by size:
755 224 185
SanDI GG'GwC_CC
Cuts at: 0 1090 1164
Size: 1090 74
ScaI AGT'ACT
Cuts at: 0 493 1164
Size: 493 671
SfcI C'TryA_G
Cuts at: 0 307 756 1152 1164
Size: 307 449 396 12
Fragments arranged by size:
449 396 307 12
SmaI CCC'GGG
Cuts at: 0 107 392 418 1164
Size: 107 285 26 746
Fragments arranged by size:
746 285 107 26
SmlI C'TyrA_G
Cuts at: 0 40 1164
Size: 40 1124
StyI C'CwwG_G
Cuts at: 0 25 167 783 1043 1164
Size: 25 142 616 260 121
Fragments arranged by size:
616 260 142 121 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 884 1037 1164
Size: 884 153 127
Fragments arranged by size:
884 153 127
TatI w'GTAC_w
Cuts at: 0 491 1164
Size: 491 673
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1012 1044 1164
Size: 1012 32 120
Fragments arranged by size:
1012 120 32
Tth111I GACn'n_nGTC
Cuts at: 0 261 1164
Size: 261 903
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 679 1041 1164
Size: 679 362 123
Fragments arranged by size:
679 362 123
XhoI C'TCGA_G
Cuts at: 0 40 1164
Size: 40 1124
XmaI C'CCGG_G
Cuts at: 0 105 390 416 1164
Size: 105 285 26 748
Fragments arranged by size:
748 285 105 26
XmnI GAAnn'nnTTC
Cuts at: 0 151 1164
Size: 151 1013
Enzymes that do cut:
AarI AflIII AlfI ApoI AvaI BanI BanII BcgI
BciVI BglI BlpI Bme1580I BmgBI BpmI Bpu10I BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BtgI BtsI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI HaeII Hin4I MmeI MscI
NaeI NcoI NgoMIV NspI PasI PflMI PpuMI PstI
SacI SanDI ScaI SfcI SmaI SmlI StyI TaqII
TatI TstI Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BclI
BfrBI BglII BmrI BmtI BplI BpuEI BsaBI BsmI
BspEI BspHI BsrGI BssHII BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EcoNI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MslI MspA1I NarI
NdeI NheI NotI NruI NsiI PacI PciI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacII SalI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI XbaI ZraI