Restriction map for
pF1KB8992
(Linear) (Six-base) MAPSORT of: KIBB8992.seq Check: 4937 from: 1 to: 603
KIBB8992.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AflII C'TTAA_G
Cuts at: 0 585 603
Size: 585 18
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 379 603
Size: 379 224
AlwNI CAG_nnn'CTG
Cuts at: 0 39 251 390 603
Size: 39 212 139 213
Fragments arranged by size:
213 212 139 39
BanI G'GyrC_C
Cuts at: 0 123 603
Size: 123 480
BlpI GC'TnA_GC
Cuts at: 0 160 603
Size: 160 443
Bme1580I G_kGCm'C
Cuts at: 0 128 358 603
Size: 128 230 245
Fragments arranged by size:
245 230 128
BmgBI CAC'GTC
Cuts at: 0 204 291 468 603
Size: 204 87 177 135
Fragments arranged by size:
204 177 135 87
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 603
Size: 322 281
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 541 603
Size: 82 459 62
Fragments arranged by size:
459 82 62
BsaAI yAC'GTr
Cuts at: 0 273 603
Size: 273 330
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 3 319 603
Size: 3 316 284
Fragments arranged by size:
316 284 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 307 421 603
Size: 237 70 114 182
Fragments arranged by size:
237 182 114 70
BsiEI CG_ry'CG
Cuts at: 0 71 229 603
Size: 71 158 374
Fragments arranged by size:
374 158 71
BsiHKAI G_wGCw'C
Cuts at: 0 89 603
Size: 89 514
Bsp1286I G_dGCh'C
Cuts at: 0 89 128 358 603
Size: 89 39 230 245
Fragments arranged by size:
245 230 89 39
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 398 603
Size: 398 205
BsrFI r'CCGG_y
Cuts at: 0 70 92 296 405 474 603
Size: 70 22 204 109 69 129
Fragments arranged by size:
204 129 109 70 69 22
BssSI C'ACGA_G
Cuts at: 0 439 603
Size: 439 164
BstAPI GCAn_nnn'nTGC
Cuts at: 0 188 390 603
Size: 188 202 213
Fragments arranged by size:
213 202 188
BstEII G'GTnAC_C
Cuts at: 0 330 603
Size: 330 273
BtgI C'CryG_G
Cuts at: 0 80 603
Size: 80 523
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 357 603
Size: 357 246
DraIII CAC_nnn'GTG
Cuts at: 0 330 603
Size: 330 273
EaeI y'GGCC_r
Cuts at: 0 68 173 210 226 452 603
Size: 68 105 37 16 226 151
Fragments arranged by size:
226 151 105 68 37 16
EagI C'GGCC_G
Cuts at: 0 68 226 603
Size: 68 158 377
Fragments arranged by size:
377 158 68
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 603
Size: 322 281
EcoNI CCTnn'n_nnAGG
Cuts at: 0 505 603
Size: 505 98
EcoO109I rG'GnC_Cy
Cuts at: 0 387 498 603
Size: 387 111 105
Fragments arranged by size:
387 111 105
HaeII r_GCGC'y
Cuts at: 0 436 603
Size: 436 167
MslI CAynn'nnrTG
Cuts at: 0 271 603
Size: 271 332
MspA1I CmG'CkG
Cuts at: 0 188 231 251 363 429 603
Size: 188 43 20 112 66 174
Fragments arranged by size:
188 174 112 66 43 20
NaeI GCC'GGC
Cuts at: 0 72 476 603
Size: 72 404 127
Fragments arranged by size:
404 127 72
NgoMIV G'CCGG_C
Cuts at: 0 70 474 603
Size: 70 404 129
Fragments arranged by size:
404 129 70
NotI GC'GGCC_GC
Cuts at: 0 226 603
Size: 226 377
PpuMI rG'GwC_Cy
Cuts at: 0 387 603
Size: 387 216
PvuII CAG'CTG
Cuts at: 0 188 429 603
Size: 188 241 174
Fragments arranged by size:
241 188 174
SfcI C'TryA_G
Cuts at: 0 243 603
Size: 243 360
SmlI C'TyrA_G
Cuts at: 0 97 556 585 603
Size: 97 459 29 18
Fragments arranged by size:
459 97 29 18
StyI C'CwwG_G
Cuts at: 0 176 603
Size: 176 427
Enzymes that do cut:
AflII AlfI AlwNI BanI BlpI Bme1580I BmgBI BpmI
BpuEI BsaAI BseRI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrFI BssSI BstAPI BstEII BtgI BtgZI DraIII EaeI
EagI Eco57MI EcoNI EcoO109I HaeII MslI MspA1I NaeI
NgoMIV NotI PpuMI PvuII SfcI SmlI StyI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BmrI BmtI BplI Bpu10I BsaI BsaBI BsaHI BsaWI
BsaXI BseYI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrGI BssHII BstBI BstXI BstYI BstZ17I Bsu36I
BtsI ClaI CspCI DraI DrdI EarI EciI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
NarI NcoI NdeI NheI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI