Restriction map for
pF1KB8995
(Linear) (Six-base) MAPSORT of: KIBB8995.seq Check: 8354 from: 1 to: 1236
KIBB8995.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 0 238 1236
Size: 238 998
AflIII A'CryG_T
Cuts at: 0 1049 1236
Size: 1049 187
AleI CACnn'nnGTG
Cuts at: 0 1015 1236
Size: 1015 221
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 67 1033 1236
Size: 67 966 203
Fragments arranged by size:
966 203 67
AlwNI CAG_nnn'CTG
Cuts at: 0 960 1236
Size: 960 276
ApaI G_GGCC'C
Cuts at: 0 40 933 1127 1236
Size: 40 893 194 109
Fragments arranged by size:
893 194 109 40
ApaLI G'TGCA_C
Cuts at: 0 1008 1236
Size: 1008 228
AvaI C'yCGr_G
Cuts at: 0 110 1167 1236
Size: 110 1057 69
Fragments arranged by size:
1057 110 69
BanII G_rGCy'C
Cuts at: 0 40 933 1127 1168 1187 1236
Size: 40 893 194 41 19 49
Fragments arranged by size:
893 194 49 41 40 19
BbvCI CC'TCA_GC
Cuts at: 0 187 1236
Size: 187 1049
BglI GCCn_nnn'nGGC
Cuts at: 0 388 448 478 508 598 658 688 718
Size: 388 60 30 30 90 60 30 30
Cuts at: 718 748 778 808 1236
Size: 30 30 30 428
Fragments arranged by size:
428 388 90 60 60 30 30 30
30 30 30 30
Bme1580I G_kGCm'C
Cuts at: 0 40 413 933 1012 1127 1236
Size: 40 373 520 79 115 109
Fragments arranged by size:
520 373 115 109 79 40
BmrI ACTGGGnnnn_n'
Cuts at: 0 43 227 791 916 1236
Size: 43 184 564 125 320
Fragments arranged by size:
564 320 184 125 43
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1143 1236
Size: 1143 93
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 586 1236
Size: 161 425 650
Fragments arranged by size:
650 425 161
Bpu10I CC'TnA_GC
Cuts at: 0 187 1236
Size: 187 1049
BsaHI Gr'CG_yC
Cuts at: 0 1177 1236
Size: 1177 59
BsaWI w'CCGG_w
Cuts at: 0 116 1236
Size: 116 1120
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 159 189 278 308 769 799 1236
Size: 159 30 89 30 461 30 437
Fragments arranged by size:
461 437 159 89 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 4 200 1236
Size: 4 196 1036
Fragments arranged by size:
1036 196 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 566 596 686 1204 1236
Size: 566 30 90 518 32
Fragments arranged by size:
566 518 90 32 30
BsiHKAI G_wGCw'C
Cuts at: 0 1012 1236
Size: 1012 224
BsmI GAATG_Cn'
Cuts at: 0 884 1236
Size: 884 352
Bsp1286I G_dGCh'C
Cuts at: 0 40 413 933 1012 1127 1168 1187 1236
Size: 40 373 520 79 115 41 19 49
Fragments arranged by size:
520 373 115 79 49 41 40 19
BstAPI GCAn_nnn'nTGC
Cuts at: 0 960 1236
Size: 960 276
BstXI CCAn_nnnn'nTGG
Cuts at: 0 315 435 495 564 684 765 1083 1236
Size: 315 120 60 69 120 81 318 153
Fragments arranged by size:
318 315 153 120 120 81 69 60
BtgI C'CryG_G
Cuts at: 0 308 338 383 413 473 533 593 623
Size: 308 30 45 30 60 60 60 30
Cuts at: 623 653 683 713 743 773 818 890 1236
Size: 30 30 30 30 30 45 72 346
Fragments arranged by size:
346 308 72 60 60 60 45 45
30 30 30 30 30 30 30 30
BtsI GCAGTG_nn'
Cuts at: 0 578 608 698 961 1236
Size: 578 30 90 263 275
Fragments arranged by size:
578 275 263 90 30
EaeI y'GGCC_r
Cuts at: 0 317 470 965 989 1236
Size: 317 153 495 24 247
Fragments arranged by size:
495 317 247 153 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1018 1236
Size: 1018 218
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 586 1236
Size: 161 425 650
Fragments arranged by size:
650 425 161
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1153 1236
Size: 1153 83
EcoO109I rG'GnC_Cy
Cuts at: 0 37 97 780 810 929 930 1123 1124
Size: 37 60 683 30 119 1 193 1
Cuts at: 1124 1146 1236
Size: 22 90
Fragments arranged by size:
683 193 119 90 60 37 30 22
1 1
HaeII r_GCGC'y
Cuts at: 0 985 1236
Size: 985 251
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 278 310 769 799 801 831 928 960
Size: 278 32 459 30 2 30 97 32
Cuts at: 960 1236
Size: 276
Fragments arranged by size:
459 278 276 97 32 32 30 30
2
HincII GTy'rAC
Cuts at: 0 915 1236
Size: 915 321
MscI TGG'CCA
Cuts at: 0 319 967 991 1236
Size: 319 648 24 245
Fragments arranged by size:
648 319 245 24
MslI CAynn'nnrTG
Cuts at: 0 682 889 946 1015 1081 1236
Size: 682 207 57 69 66 155
Fragments arranged by size:
682 207 155 69 66 57
MspA1I CmG'CkG
Cuts at: 0 57 1236
Size: 57 1179
NcoI C'CATG_G
Cuts at: 0 593 623 653 683 773 818 890 1236
Size: 593 30 30 30 90 45 72 346
Fragments arranged by size:
593 346 90 72 45 30 30 30
NspI r_CATG'y
Cuts at: 0 1053 1236
Size: 1053 183
PasI CC'CwG_GG
Cuts at: 0 1187 1236
Size: 1187 49
PciI A'CATG_T
Cuts at: 0 1049 1236
Size: 1049 187
PpuMI rG'GwC_Cy
Cuts at: 0 97 1236
Size: 97 1139
PspOMI G'GGCC_C
Cuts at: 0 36 929 1123 1236
Size: 36 893 194 113
Fragments arranged by size:
893 194 113 36
PvuII CAG'CTG
Cuts at: 0 57 1236
Size: 57 1179
RsrII CG'GwC_CG
Cuts at: 0 1026 1236
Size: 1026 210
SanDI GG'GwC_CC
Cuts at: 0 97 1236
Size: 97 1139
SmaI CCC'GGG
Cuts at: 0 112 1169 1236
Size: 112 1057 67
Fragments arranged by size:
1057 112 67
SrfI GCCC'GGGC
Cuts at: 0 1169 1236
Size: 1169 67
StuI AGG'CCT
Cuts at: 0 563 1236
Size: 563 673
StyI C'CwwG_G
Cuts at: 0 92 593 623 653 683 773 818 890
Size: 92 501 30 30 30 90 45 72
Cuts at: 890 1154 1236
Size: 264 82
Fragments arranged by size:
501 264 92 90 82 72 45 30
30 30
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 975 1236
Size: 975 261
XmaI C'CCGG_G
Cuts at: 0 110 1167 1236
Size: 110 1057 69
Fragments arranged by size:
1057 110 69
Enzymes that do cut:
AccI AflIII AleI AlfI AlwNI ApaI ApaLI AvaI
BanII BbvCI BglI Bme1580I BmrI BplI BpmI Bpu10I
BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I
BstAPI BstXI BtgI BtsI EaeI EciI Eco57MI EcoNI
EcoO109I HaeII Hin4I HincII MscI MslI MspA1I NcoI
NspI PasI PciI PpuMI PspOMI PvuII RsrII SanDI
SmaI SrfI StuI StyI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbsI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BmtI BpuEI BsaI BsaAI BsaBI
BseYI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstYI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EarI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PstI PvuI SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SspI SwaI TaqII TatI
TstI Tth111I XbaI XhoI XmnI ZraI