Restriction map for
pF1KB9000
(Linear) (Six-base) MAPSORT of: KIBB9000.seq Check: 8198 from: 1 to: 1254
KIBB9000.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 381 395 1254
Size: 381 14 859
Fragments arranged by size:
859 381 14
AatII G_ACGT'C
Cuts at: 0 250 1254
Size: 250 1004
Acc65I G'GTAC_C
Cuts at: 0 697 1254
Size: 697 557
AflIII A'CryG_T
Cuts at: 0 1043 1254
Size: 1043 211
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 232 1254
Size: 232 1022
AlwNI CAG_nnn'CTG
Cuts at: 0 315 1254
Size: 315 939
ApaI G_GGCC'C
Cuts at: 0 32 1254
Size: 32 1222
ApoI r'AATT_y
Cuts at: 0 1114 1234 1254
Size: 1114 120 20
Fragments arranged by size:
1114 120 20
AvaI C'yCGr_G
Cuts at: 0 33 103 848 1254
Size: 33 70 745 406
Fragments arranged by size:
745 406 70 33
BamHI G'GATC_C
Cuts at: 0 948 1254
Size: 948 306
BanI G'GyrC_C
Cuts at: 0 678 697 912 1254
Size: 678 19 215 342
Fragments arranged by size:
678 342 215 19
BanII G_rGCy'C
Cuts at: 0 32 1254
Size: 32 1222
BbeI G_GCGC'C
Cuts at: 0 682 916 1254
Size: 682 234 338
Fragments arranged by size:
682 338 234
BbsI GAAGACnn'nnnn_
Cuts at: 0 555 754 1155 1254
Size: 555 199 401 99
Fragments arranged by size:
555 401 199 99
BbvCI CC'TCA_GC
Cuts at: 0 277 1254
Size: 277 977
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 114 148 1254
Size: 114 34 1106
Fragments arranged by size:
1106 114 34
BglI GCCn_nnn'nGGC
Cuts at: 0 172 292 1254
Size: 172 120 962
Fragments arranged by size:
962 172 120
BlpI GC'TnA_GC
Cuts at: 0 529 541 1254
Size: 529 12 713
Fragments arranged by size:
713 529 12
Bme1580I G_kGCm'C
Cuts at: 0 32 1254
Size: 32 1222
BmgBI CAC'GTC
Cuts at: 0 132 1254
Size: 132 1122
BmrI ACTGGGnnnn_n'
Cuts at: 0 1015 1254
Size: 1015 239
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 178 1254
Size: 27 151 1076
Fragments arranged by size:
1076 151 27
Bpu10I CC'TnA_GC
Cuts at: 0 277 1254
Size: 277 977
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 1254
Size: 454 800
BsaAI yAC'GTr
Cuts at: 0 648 1254
Size: 648 606
BsaHI Gr'CG_yC
Cuts at: 0 235 247 679 754 913 1254
Size: 235 12 432 75 159 341
Fragments arranged by size:
432 341 235 159 75 12
BsaWI w'CCGG_w
Cuts at: 0 230 1254
Size: 230 1024
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 157 1254
Size: 157 1097
BseYI C'CCAG_C
Cuts at: 0 198 329 608 908 1254
Size: 198 131 279 300 346
Fragments arranged by size:
346 300 279 198 131
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 794 1254
Size: 211 583 460
Fragments arranged by size:
583 460 211
BsiEI CG_ry'CG
Cuts at: 0 444 518 1254
Size: 444 74 736
Fragments arranged by size:
736 444 74
BsiHKAI G_wGCw'C
Cuts at: 0 547 1254
Size: 547 707
BsmI GAATG_Cn'
Cuts at: 0 301 1254
Size: 301 953
BsmBI CGTCTCn'nnnn_
Cuts at: 0 10 1254
Size: 10 1244
Bsp1286I G_dGCh'C
Cuts at: 0 32 547 1254
Size: 32 515 707
Fragments arranged by size:
707 515 32
BspHI T'CATG_A
Cuts at: 0 431 506 1254
Size: 431 75 748
Fragments arranged by size:
748 431 75
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 381 395 709 1254
Size: 381 14 314 545
Fragments arranged by size:
545 381 314 14
BsrBI CCG'CTC
Cuts at: 0 468 497 1254
Size: 468 29 757
Fragments arranged by size:
757 468 29
BsrFI r'CCGG_y
Cuts at: 0 13 67 1254
Size: 13 54 1187
Fragments arranged by size:
1187 54 13
BssHII G'CGCG_C
Cuts at: 0 490 1254
Size: 490 764
BssSI C'ACGA_G
Cuts at: 0 638 1254
Size: 638 616
BstYI r'GATC_y
Cuts at: 0 857 948 1254
Size: 857 91 306
Fragments arranged by size:
857 306 91
BtgI C'CryG_G
Cuts at: 0 49 296 351 683 741 1254
Size: 49 247 55 332 58 513
Fragments arranged by size:
513 332 247 58 55 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 435 1254
Size: 435 819
BtsI GCAGTG_nn'
Cuts at: 0 995 1254
Size: 995 259
DraIII CAC_nnn'GTG
Cuts at: 0 823 1254
Size: 823 431
EaeI y'GGCC_r
Cuts at: 0 62 149 191 284 293 515 1056 1254
Size: 62 87 42 93 9 222 541 198
Fragments arranged by size:
541 222 198 93 87 62 42 9
EagI C'GGCC_G
Cuts at: 0 515 1254
Size: 515 739
EarI CTCTTCn'nnn_
Cuts at: 0 268 1254
Size: 268 986
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 178 1254
Size: 27 151 1076
Fragments arranged by size:
1076 151 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 106 1254
Size: 106 1148
EcoO109I rG'GnC_Cy
Cuts at: 0 28 29 1254
Size: 28 1 1225
Fragments arranged by size:
1225 28 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1172 1254
Size: 1172 82
FspI TGC'GCA
Cuts at: 0 136 1254
Size: 136 1118
HaeII r_GCGC'y
Cuts at: 0 74 682 916 1254
Size: 74 608 234 338
Fragments arranged by size:
608 338 234 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 940 972 1254
Size: 940 32 282
Fragments arranged by size:
940 282 32
HindIII A'AGCT_T
Cuts at: 0 1083 1254
Size: 1083 171
KasI G'GCGC_C
Cuts at: 0 678 912 1254
Size: 678 234 342
Fragments arranged by size:
678 342 234
KpnI G_GTAC'C
Cuts at: 0 701 1254
Size: 701 553
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 1254
Size: 294 960
MscI TGG'CCA
Cuts at: 0 64 151 1058 1254
Size: 64 87 907 196
Fragments arranged by size:
907 196 87 64
MslI CAynn'nnrTG
Cuts at: 0 454 673 931 1254
Size: 454 219 258 323
Fragments arranged by size:
454 323 258 219
MspA1I CmG'CkG
Cuts at: 0 353 743 1254
Size: 353 390 511
Fragments arranged by size:
511 390 353
NarI GG'CG_CC
Cuts at: 0 679 913 1254
Size: 679 234 341
Fragments arranged by size:
679 341 234
NcoI C'CATG_G
Cuts at: 0 49 1254
Size: 49 1205
NotI GC'GGCC_GC
Cuts at: 0 515 1254
Size: 515 739
NspI r_CATG'y
Cuts at: 0 258 405 423 1047 1254
Size: 258 147 18 624 207
Fragments arranged by size:
624 258 207 147 18
PasI CC'CwG_GG
Cuts at: 0 629 1254
Size: 629 625
PciI A'CATG_T
Cuts at: 0 1043 1254
Size: 1043 211
PflMI CCAn_nnn'nTGG
Cuts at: 0 50 1254
Size: 50 1204
PspOMI G'GGCC_C
Cuts at: 0 28 1254
Size: 28 1226
PstI C_TGCA'G
Cuts at: 0 392 1254
Size: 392 862
SacII CC_GC'GG
Cuts at: 0 354 744 1254
Size: 354 390 510
Fragments arranged by size:
510 390 354
SbfI CC_TGCA'GG
Cuts at: 0 392 1254
Size: 392 862
ScaI AGT'ACT
Cuts at: 0 556 1254
Size: 556 698
SfcI C'TryA_G
Cuts at: 0 388 1254
Size: 388 866
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 292 1254
Size: 292 962
SfoI GGC'GCC
Cuts at: 0 680 914 1254
Size: 680 234 340
Fragments arranged by size:
680 340 234
SgrAI Cr'CCGG_yG
Cuts at: 0 67 1254
Size: 67 1187
SmaI CCC'GGG
Cuts at: 0 850 1254
Size: 850 404
SmlI C'TyrA_G
Cuts at: 0 469 1254
Size: 469 785
SphI G_CATG'C
Cuts at: 0 258 1254
Size: 258 996
StyI C'CwwG_G
Cuts at: 0 49 593 1254
Size: 49 544 661
Fragments arranged by size:
661 544 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 513 982 1254
Size: 513 469 272
Fragments arranged by size:
513 469 272
TatI w'GTAC_w
Cuts at: 0 554 1254
Size: 554 700
Tth111I GACn'n_nGTC
Cuts at: 0 753 1254
Size: 753 501
XmaI C'CCGG_G
Cuts at: 0 848 1254
Size: 848 406
ZraI GAC'GTC
Cuts at: 0 248 1254
Size: 248 1006
Enzymes that do cut:
AarI AatII Acc65I AflIII AlfI AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BssHII
BssSI BstYI BtgI BtgZI BtsI DraIII EaeI EagI
EarI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I
HindIII KasI KpnI MmeI MscI MslI MspA1I NarI
NcoI NotI NspI PasI PciI PflMI PspOMI PstI
SacII SbfI ScaI SfcI SfiI SfoI SgrAI SmaI
SmlI SphI StyI TaqII TatI Tth111I XmaI ZraI
Enzymes that do not cut:
AccI AclI AcuI AfeI AflII AgeI AhdI AleI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BciVI
BclI BfrBI BglII BmtI BplI BsaI BsaBI BsaXI
BsiWI BspEI BsrDI BsrGI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraI DrdI EciI EcoICRI
EcoRI EcoRV FseI FspAI HincII HpaI MfeI MluI
NaeI NdeI NgoMIV NheI NruI NsiI PacI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacI SalI SanDI SapI SexAI
SgfI SnaBI SpeI SrfI SspI StuI SwaI TstI
XbaI XcmI XhoI XmnI