Restriction map for
pF1KB9016
(Linear) (Six-base) MAPSORT of: KIBB9016.seq Check: 1166 from: 1 to: 783
KIBB9016.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AccI GT'mk_AC
Cuts at: 0 149 719 783
Size: 149 570 64
Fragments arranged by size:
570 149 64
ApaI G_GGCC'C
Cuts at: 0 608 783
Size: 608 175
BanI G'GyrC_C
Cuts at: 0 352 783
Size: 352 431
BanII G_rGCy'C
Cuts at: 0 608 783
Size: 608 175
BbeI G_GCGC'C
Cuts at: 0 356 783
Size: 356 427
BbsI GAAGACnn'nnnn_
Cuts at: 0 781 783
Size: 781 2
BciVI GTATCCnnnnn_n'
Cuts at: 0 565 783
Size: 565 218
BclI T'GATC_A
Cuts at: 0 75 783
Size: 75 708
BglI GCCn_nnn'nGGC
Cuts at: 0 503 783
Size: 503 280
Bme1580I G_kGCm'C
Cuts at: 0 608 783
Size: 608 175
BmrI ACTGGGnnnn_n'
Cuts at: 0 410 783
Size: 410 373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 384 783
Size: 364 20 399
Fragments arranged by size:
399 364 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 783
Size: 377 406
BsaAI yAC'GTr
Cuts at: 0 81 576 783
Size: 81 495 207
Fragments arranged by size:
495 207 81
BsaHI Gr'CG_yC
Cuts at: 0 353 783
Size: 353 430
BsaWI w'CCGG_w
Cuts at: 0 618 762 783
Size: 618 144 21
Fragments arranged by size:
618 144 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 26 56 783
Size: 26 30 727
Fragments arranged by size:
727 30 26
BseYI C'CCAG_C
Cuts at: 0 308 445 783
Size: 308 137 338
Fragments arranged by size:
338 308 137
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 783
Size: 598 185
Bsp1286I G_dGCh'C
Cuts at: 0 608 783
Size: 608 175
BspHI T'CATG_A
Cuts at: 0 209 227 783
Size: 209 18 556
Fragments arranged by size:
556 209 18
BssSI C'ACGA_G
Cuts at: 0 729 783
Size: 729 54
BtgI C'CryG_G
Cuts at: 0 566 608 783
Size: 566 42 175
Fragments arranged by size:
566 175 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 47 307 783
Size: 47 260 476
Fragments arranged by size:
476 260 47
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 384 783
Size: 364 20 399
Fragments arranged by size:
399 364 20
EcoO109I rG'GnC_Cy
Cuts at: 0 452 544 783
Size: 452 92 239
Fragments arranged by size:
452 239 92
HaeII r_GCGC'y
Cuts at: 0 7 356 689 783
Size: 7 349 333 94
Fragments arranged by size:
349 333 94 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 58 395 427 625 657 783
Size: 26 32 337 32 198 32 126
Fragments arranged by size:
337 198 126 32 32 32 26
HincII GTy'rAC
Cuts at: 0 335 783
Size: 335 448
KasI G'GCGC_C
Cuts at: 0 352 783
Size: 352 431
MslI CAynn'nnrTG
Cuts at: 0 64 783
Size: 64 719
MspA1I CmG'CkG
Cuts at: 0 372 449 626 783
Size: 372 77 177 157
Fragments arranged by size:
372 177 157 77
NarI GG'CG_CC
Cuts at: 0 353 783
Size: 353 430
NcoI C'CATG_G
Cuts at: 0 566 608 783
Size: 566 42 175
Fragments arranged by size:
566 175 42
NspI r_CATG'y
Cuts at: 0 444 726 783
Size: 444 282 57
Fragments arranged by size:
444 282 57
PasI CC'CwG_GG
Cuts at: 0 601 783
Size: 601 182
PfoI T'CCnGG_A
Cuts at: 0 540 783
Size: 540 243
PmlI CAC'GTG
Cuts at: 0 81 783
Size: 81 702
PpuMI rG'GwC_Cy
Cuts at: 0 544 783
Size: 544 239
PspOMI G'GGCC_C
Cuts at: 0 604 783
Size: 604 179
PstI C_TGCA'G
Cuts at: 0 654 783
Size: 654 129
SfcI C'TryA_G
Cuts at: 0 650 783
Size: 650 133
SfoI GGC'GCC
Cuts at: 0 354 783
Size: 354 429
SmlI C'TyrA_G
Cuts at: 0 392 783
Size: 392 391
StyI C'CwwG_G
Cuts at: 0 182 566 608 662 783
Size: 182 384 42 54 121
Fragments arranged by size:
384 182 121 54 42
Enzymes that do cut:
AccI ApaI BanI BanII BbeI BbsI BciVI BclI
BglI Bme1580I BmrI BpmI BpuEI BsaAI BsaHI BsaWI
BsaXI BseYI BsgI Bsp1286I BspHI BssSI BtgI EciI
Eco57MI EcoO109I HaeII Hin4I HincII KasI MslI MspA1I
NarI NcoI NspI PasI PfoI PmlI PpuMI PspOMI
PstI SfcI SfoI SmlI StyI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BbvCI
BcgI BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I
BsaI BsaBI BseRI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI MmeI MscI NaeI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PflMI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI