Restriction map for pF1KB9022
 (Linear) (Six-base) MAPSORT of: KIBB9022.seq  Check: 3040  from: 1  to: 5136



KIBB9022.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:38  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    4999    5136 
   Size:       4999     137
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2476    2890    3334    3370    3775    5136 
   Size:       2476     414     444      36     405    1361

  Fragments arranged by size: 

                2476    1361     444     414     405      36
 
AhdI GACnn_n'nnGTC

Cuts at:      0    4211    5136 
   Size:       4211     925
 
AleI CACnn'nnGTG

Cuts at:      0    1990    5136 
   Size:       1990    3146
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    4672    4704    5136 
   Size:       4672      32     432

  Fragments arranged by size: 

                4672     432      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2134    3637    3718    3907    4590    4766    5021    5111 
   Size:       2134    1503      81     189     683     176     255      90

Cuts at:   5111    5136 
   Size:         25

  Fragments arranged by size: 

                2134    1503     683     255     189     176      90      81
                  25
 
ApaI G_GGCC'C

Cuts at:      0     202     383     446     482    1034    1110    1270    1795 
   Size:        202     181      63      36     552      76     160     525

Cuts at:   1795    1858    1945    2054    2153    2297    2555    2924    3889 
   Size:         63      87     109      99     144     258     369     965

Cuts at:   3889    3998    4043    4262    4270    4316    5136 
   Size:        109      45     219       8      46     820

  Fragments arranged by size: 

                 965     820     552     525     369     258     219     202
                 181     160     144     109     109      99      87      76
                  63      63      46      45      36       8
 
ApaLI G'TGCA_C

Cuts at:      0    4403    5136 
   Size:       4403     733
 
ApoI r'AATT_y

Cuts at:      0     353    3291    5136 
   Size:        353    2938    1845

  Fragments arranged by size: 

                2938    1845     353
 
AvaI C'yCGr_G

Cuts at:      0     240     366     475     998    1092    1146    1309    1702 
   Size:        240     126     109     523      94      54     163     393

Cuts at:   1702    2216    2341    2746    2818    4144    4162    4425    5038 
   Size:        514     125     405      72    1326      18     263     613

Cuts at:   5038    5136 
   Size:         98

  Fragments arranged by size: 

                1326     613     523     514     405     393     263     240
                 163     126     125     109      98      94      72      54
                  18
 
BamHI G'GATC_C

Cuts at:      0    2221    2257    3581    3625    5136 
   Size:       2221      36    1324      44    1511

  Fragments arranged by size: 

                2221    1511    1324      44      36
 
BanI G'GyrC_C

Cuts at:      0     343    1078    2095    3217    3328    3607    3834    3852 
   Size:        343     735    1017    1122     111     279     227      18

Cuts at:   3852    4138    4242    4987    5136 
   Size:        286     104     745     149

  Fragments arranged by size: 

                1122    1017     745     735     343     286     279     227
                 149     111     104      18
 
BanII G_rGCy'C

Cuts at:      0     202     285     383     446     482     636    1034    1110 
   Size:        202      83      98      63      36     154     398      76

Cuts at:   1110    1270    1316    1410    1592    1745    1795    1858    1945 
   Size:        160      46      94     182     153      50      63      87

Cuts at:   1945    2054    2153    2297    2337    2538    2555    2924    3549 
   Size:        109      99     144      40     201      17     369     625

Cuts at:   3549    3756    3784    3889    3954    3998    4043    4161    4262 
   Size:        207      28     105      65      44      45     118     101

Cuts at:   4262    4270    4299    4316    4426    4505    4606    5059    5136 
   Size:          8      29      17     110      79     101     453      77

  Fragments arranged by size: 

                 625     453     398     369     207     202     201     182
                 160     154     153     144     118     110     109     105
                 101     101      99      98      94      87      83      79
                  77      76      65      63      63      50      46      45
                  44      40      36      29      28      17      17       8
            
 
BbeI G_GCGC'C

Cuts at:      0    3856    5136 
   Size:       3856    1280
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1474    3309    5136 
   Size:       1474    1835    1827

  Fragments arranged by size: 

                1835    1827    1474
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    3001    3037    4534    5136 
   Size:       3001      36    1497     602

  Fragments arranged by size: 

                3001    1497     602      36
 
BglI GCCn_nnn'nGGC

Cuts at:      0     477     974    1568    1766    1877    1886    1949    2246 
   Size:        477     497     594     198     111       9      63     297

Cuts at:   2246    2292    2468    2495    2559    2639    2997    3081    3242 
   Size:         46     176      27      64      80     358      84     161

Cuts at:   3242    4517    5136 
   Size:       1275     619

  Fragments arranged by size: 

                1275     619     594     497     477     358     297     198
                 176     161     111      84      80      64      63      46
                  27       9
 
BlpI GC'TnA_GC

Cuts at:      0    2336    5136 
   Size:       2336    2800
 
Bme1580I G_kGCm'C

Cuts at:      0     202     383     446     482    1034    1110    1270    1703 
   Size:        202     181      63      36     552      76     160     433

Cuts at:   1703    1795    1858    1945    2054    2153    2297    2555    2924 
   Size:         92      63      87     109      99     144     258     369

Cuts at:   2924    3222    3331    3837    3889    3998    4043    4143    4245 
   Size:        298     109     506      52     109      45     100     102

Cuts at:   4245    4262    4270    4316    4407    4513    5136 
   Size:         17       8      46      91     106     623

  Fragments arranged by size: 

                 623     552     506     433     369     298     258     202
                 181     160     144     109     109     109     106     102
                 100      99      92      91      87      76      63      63
                  52      46      45      36      17       8
 
BmgBI CAC'GTC

Cuts at:      0     178     312    5136 
   Size:        178     134    4824

  Fragments arranged by size: 

                4824     178     134
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1876    3479    5136 
   Size:       1876    1603    1657

  Fragments arranged by size: 

                1876    1657    1603
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     578     620    1283    1424    1781    1826    1925    2339 
   Size:        578      42     663     141     357      45      99     414

Cuts at:   2339    2495    3437    3461    3740    3938    4283    4554    5136 
   Size:        156     942      24     279     198     345     271     582

  Fragments arranged by size: 

                 942     663     582     578     414     357     345     279
                 271     198     156     141      99      45      42      24
            
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     945    1576    5062    5136 
   Size:        945     631    3486      74

  Fragments arranged by size: 

                3486     945     631      74
 
BsaI GGTCTCn'nnnn_

Cuts at:      0       2     636    1016    1136    1232    1547    2656    2808 
   Size:          2     634     380     120      96     315    1109     152

Cuts at:   2808    3644    3737    4027    4115    5136 
   Size:        836      93     290      88    1021

  Fragments arranged by size: 

                1109    1021     836     634     380     315     290     152
                 120      96      93      88       2
 
BsaBI GATnn'nnATC

Cuts at:      0     977    1187    5136 
   Size:        977     210    3949

  Fragments arranged by size: 

                3949     977     210
 
BsaHI Gr'CG_yC

Cuts at:      0    1688    1910    3853    5136 
   Size:       1688     222    1943    1283

  Fragments arranged by size: 

                1943    1688    1283     222
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     456     486    1556    1586    2610    2640    3459    3489 
   Size:        456      30    1070      30    1024      30     819      30

Cuts at:   3489    4492    4522    5136 
   Size:       1003      30     614

  Fragments arranged by size: 

                1070    1024    1003     819     614     456      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     544    1209    2681    5136 
   Size:        544     665    1472    2455

  Fragments arranged by size: 

                2455    1472     665     544
 
BseYI C'CCAG_C

Cuts at:      0      54    1438    3495    4309    4836    4840    5136 
   Size:         54    1384    2057     814     527       4     296

  Fragments arranged by size: 

                2057    1384     814     527     296      54       4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2252    5136 
   Size:       2252    2884
 
BsiEI CG_ry'CG

Cuts at:      0     597    5136 
   Size:        597    4539
 
BsiHKAI G_wGCw'C

Cuts at:      0     636    1620    2337    2538    3549    3756    3954    4407 
   Size:        636     984     717     201    1011     207     198     453

Cuts at:   4407    5136 
   Size:        729

  Fragments arranged by size: 

                1011     984     729     717     636     453     207     201
                 198
 
BsmI GAATG_Cn'

Cuts at:      0    1450    2738    3041    4975    5136 
   Size:       1450    1288     303    1934     161

  Fragments arranged by size: 

                1934    1450    1288     303     161
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1901    2162    4889    5136 
   Size:       1901     261    2727     247

  Fragments arranged by size: 

                2727    1901     261     247
 
Bsp1286I G_dGCh'C

Cuts at:      0     202     285     383     446     482     636    1034    1110 
   Size:        202      83      98      63      36     154     398      76

Cuts at:   1110    1270    1316    1410    1592    1620    1703    1745    1795 
   Size:        160      46      94     182      28      83      42      50

Cuts at:   1795    1858    1945    2054    2153    2297    2337    2538    2555 
   Size:         63      87     109      99     144      40     201      17

Cuts at:   2555    2924    3222    3331    3549    3756    3784    3837    3889 
   Size:        369     298     109     218     207      28      53      52

Cuts at:   3889    3954    3998    4043    4143    4161    4245    4262    4270 
   Size:         65      44      45     100      18      84      17       8

Cuts at:   4270    4299    4316    4407    4426    4505    4513    4606    5059 
   Size:         29      17      91      19      79       8      93     453

Cuts at:   5059    5136 
   Size:         77

  Fragments arranged by size: 

                 453     398     369     298     218     207     202     201
                 182     160     154     144     109     109     100      99
                  98      94      93      91      87      84      83      83
                  79      77      76      65      63      63      53      52
                  50      46      45      44      42      40      36      29
                  28      28      19      18      17      17      17       8
                   8
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1053    2164    4999    5136 
   Size:       1053    1111    2835     137

  Fragments arranged by size: 

                2835    1111    1053     137
 
BsrBI CCG'CTC

Cuts at:      0    1674    3324    5136 
   Size:       1674    1650    1812

  Fragments arranged by size: 

                1812    1674    1650
 
BsrDI GCAATG_nn'

Cuts at:      0    4980    5136 
   Size:       4980     156
 
BsrFI r'CCGG_y

Cuts at:      0      25    1912    5083    5136 
   Size:         25    1887    3171      53

  Fragments arranged by size: 

                3171    1887      53      25
 
BsrGI T'GTAC_A

Cuts at:      0    1453    5136 
   Size:       1453    3683
 
BssHII G'CGCG_C

Cuts at:      0      15    5136 
   Size:         15    5121
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1078    5136 
   Size:       1078    4058
 
BstEII G'GTnAC_C

Cuts at:      0     913    1087    3685    5136 
   Size:        913     174    2598    1451

  Fragments arranged by size: 

                2598    1451     913     174
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1424    4844    5136 
   Size:       1424    3420     292

  Fragments arranged by size: 

                3420    1424     292
 
BstYI r'GATC_y

Cuts at:      0    2221    2257    3581    3625    5136 
   Size:       2221      36    1324      44    1511

  Fragments arranged by size: 

                2221    1511    1324      44      36
 
BtgI C'CryG_G

Cuts at:      0      68    1410    2347    3576    3585    3645    4730    4983 
   Size:         68    1342     937    1229       9      60    1085     253

Cuts at:   4983    5136 
   Size:        153

  Fragments arranged by size: 

                1342    1229    1085     937     253     153      68      60
                   9
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     768    1751    4776    4782    5136 
   Size:        768     983    3025       6     354

  Fragments arranged by size: 

                3025     983     768     354       6
 
BtsI GCAGTG_nn'

Cuts at:      0     147     517     843    2240    4094    4706    4830    5136 
   Size:        147     370     326    1397    1854     612     124     306

  Fragments arranged by size: 

                1854    1397     612     370     326     306     147     124
            
 
DraI TTT'AAA

Cuts at:      0    2718    2898    5136 
   Size:       2718     180    2238

  Fragments arranged by size: 

                2718    2238     180
 
DraIII CAC_nnn'GTG

Cuts at:      0    3841    5136 
   Size:       3841    1295
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     620    3118    5136 
   Size:        620    2498    2018

  Fragments arranged by size: 

                2498    2018     620
 
EaeI y'GGCC_r

Cuts at:      0      23     594    1560    2487    2541    3087    3538    4392 
   Size:         23     571     966     927      54     546     451     854

Cuts at:   4392    4733    4787    4908    5136 
   Size:        341      54     121     228

  Fragments arranged by size: 

                 966     927     854     571     546     451     341     228
                 121      54      54      23
 
EagI C'GGCC_G

Cuts at:      0     594    5136 
   Size:        594    4542
 
EarI CTCTTCn'nnn_

Cuts at:      0     126    1365    3920    4317    5136 
   Size:        126    1239    2555     397     819

  Fragments arranged by size: 

                2555    1239     819     397     126
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    3516    4863    5055    5136 
   Size:       3516    1347     192      81

  Fragments arranged by size: 

                3516    1347     192      81
 
EcoICRI GAG'CTC

Cuts at:      0     634    2335    2536    3547    3754    3952    5136 
   Size:        634    1701     201    1011     207     198    1184

  Fragments arranged by size: 

                1701    1184    1011     634     207     201     198
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     578     620    1283    1424    1781    1826    1925    2339 
   Size:        578      42     663     141     357      45      99     414

Cuts at:   2339    2476    2495    2890    3334    3370    3437    3461    3740 
   Size:        137      19     395     444      36      67      24     279

Cuts at:   3740    3775    3938    4283    4554    5136 
   Size:         35     163     345     271     582

  Fragments arranged by size: 

                 663     582     578     444     414     395     357     345
                 279     271     163     141     137      99      67      45
                  42      36      35      24      19
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     167    5136 
   Size:        167    4969
 
EcoO109I rG'GnC_Cy

Cuts at:      0     199     298     442     443     479     586     745     985 
   Size:        199      99     144       1      36     107     159     240

Cuts at:    985    1003    1030    1031    1106    1159    1219    1266    1294 
   Size:         18      27       1      75      53      60      47      28

Cuts at:   1294    1303    1498    1768    1791    1792    1803    1855    1942 
   Size:          9     195     270      23       1      11      52      87

Cuts at:   1942    1961    2050    2077    2086    2149    2194    2212    2266 
   Size:         19      89      27       9      63      45      18      54

Cuts at:   2266    2294    2310    2407    2479    2551    2552    2607    2741 
   Size:         28      16      97      72      72       1      55     134

Cuts at:   2741    2913    2921    2989    3427    3667    3715    3885    3995 
   Size:        172       8      68     438     240      48     170     110

Cuts at:   3995    4039    4102    4111    4213    4231    4258    4259    4266 
   Size:         44      63       9     102      18      27       1       7

Cuts at:   4266    4267    4313    4366    4587    4782    5136 
   Size:          1      46      53     221     195     354

  Fragments arranged by size: 

                 438     354     270     240     240     221     199     195
                 195     172     170     159     144     134     110     107
                 102      99      97      89      87      75      72      72
                  68      63      63      60      55      54      53      53
                  52      48      47      46      45      44      36      28
                  28      27      27      27      23      19      18      18
                  18      16      11       9       9       9       8       7
                   1       1       1       1       1       1
 
EcoRI G'AATT_C

Cuts at:      0     353    5136 
   Size:        353    4783
 
EcoRV GAT'ATC

Cuts at:      0     544    5136 
   Size:        544    4592
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    3375    5034    5136 
   Size:       3375    1659     102

  Fragments arranged by size: 

                3375    1659     102
 
FseI GG_CCGG'CC

Cuts at:      0      29    5087    5136 
   Size:         29    5058      49

  Fragments arranged by size: 

                5058      49      29
 
HaeII r_GCGC'y

Cuts at:      0    2627    3856    5136 
   Size:       2627    1229    1280

  Fragments arranged by size: 

                2627    1280    1229
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    2752    2784    3459    3491    4197    4229    5136 
   Size:       2752      32     675      32     706      32     907

  Fragments arranged by size: 

                2752     907     706     675      32      32      32
 
KasI G'GCGC_C

Cuts at:      0    3852    5136 
   Size:       3852    1284
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     609    1986    3845    4221    5136 
   Size:        609    1377    1859     376     915

  Fragments arranged by size: 

                1859    1377     915     609     376
 
MscI TGG'CCA

Cuts at:      0    2543    4394    4910    5136 
   Size:       2543    1851     516     226

  Fragments arranged by size: 

                2543    1851     516     226
 
MslI CAynn'nnrTG

Cuts at:      0    1990    5136 
   Size:       1990    3146
 
MspA1I CmG'CkG

Cuts at:      0    1412    3587    4716    4766    4840    4934    4985    5111 
   Size:       1412    2175    1129      50      74      94      51     126

Cuts at:   5111    5136 
   Size:         25

  Fragments arranged by size: 

                2175    1412    1129     126      94      74      51      50
                  25
 
NaeI GCC'GGC

Cuts at:      0      27    1914    5085    5136 
   Size:         27    1887    3171      51

  Fragments arranged by size: 

                3171    1887      51      27
 
NarI GG'CG_CC

Cuts at:      0    3853    5136 
   Size:       3853    1283
 
NgoMIV G'CCGG_C

Cuts at:      0      25    1912    5083    5136 
   Size:         25    1887    3171      53

  Fragments arranged by size: 

                3171    1887      53      25
 
NspI r_CATG'y

Cuts at:      0     610    2799    5136 
   Size:        610    2189    2337

  Fragments arranged by size: 

                2337    2189     610
 
PasI CC'CwG_GG

Cuts at:      0     204     383     420     601    1027    1102    1384    1576 
   Size:        204     179      37     181     426      75     282     192

Cuts at:   1576    1585    2153    2200    2299    2611    2917    3049    3067 
   Size:          9     568      47      99     312     306     132      18

Cuts at:   3067    3151    3223    3397    3964    3999    4043    4218    4237 
   Size:         84      72     174     567      35      44     175      19

Cuts at:   4237    4263    5136 
   Size:         26     873

  Fragments arranged by size: 

                 873     568     567     426     312     306     282     204
                 192     181     179     175     174     132      99      84
                  75      72      47      44      37      35      26      19
                  18       9
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    3977    4590    4917    5136 
   Size:       3977     613     327     219

  Fragments arranged by size: 

                3977     613     327     219
 
PfoI T'CCnGG_A

Cuts at:      0     357     900    1299    1485    1620    1815    1842    2394 
   Size:        357     543     399     186     135     195      27     552

Cuts at:   2394    2511    2700    3432    3567    3621    3918    3954    5136 
   Size:        117     189     732     135      54     297      36    1182

  Fragments arranged by size: 

                1182     732     552     543     399     357     297     195
                 189     186     135     135     117      54      36      27
            
 
PpuMI rG'GwC_Cy

Cuts at:      0     298     586     745    1003    1159    1219    1303    1498 
   Size:        298     288     159     258     156      60      84     195

Cuts at:   1498    1803    1961    2077    2194    2212    2266    2479    2607 
   Size:        305     158     116     117      18      54     213     128

Cuts at:   2607    2741    2913    4111    4213    4366    4587    5136 
   Size:        134     172    1198     102     153     221     549

  Fragments arranged by size: 

                1198     549     305     298     288     258     221     213
                 195     172     159     158     156     153     134     128
                 117     116     102      84      60      54      18
 
PshAI GACnn'nnGTC

Cuts at:      0     431    2174    3418    5136 
   Size:        431    1743    1244    1718

  Fragments arranged by size: 

                1743    1718    1244     431
 
PspOMI G'GGCC_C

Cuts at:      0     198     379     442     478    1030    1106    1266    1791 
   Size:        198     181      63      36     552      76     160     525

Cuts at:   1791    1854    1941    2050    2149    2293    2551    2920    3885 
   Size:         63      87     109      99     144     258     369     965

Cuts at:   3885    3994    4039    4258    4266    4312    5136 
   Size:        109      45     219       8      46     824

  Fragments arranged by size: 

                 965     824     552     525     369     258     219     198
                 181     160     144     109     109      99      87      76
                  63      63      46      45      36       8
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1821    1853    5136 
   Size:       1821      32    3283

  Fragments arranged by size: 

                3283    1821      32
 
PstI C_TGCA'G

Cuts at:      0     251    5136 
   Size:        251    4885
 
PvuII CAG'CTG

Cuts at:      0    4840    4934    5136 
   Size:       4840      94     202

  Fragments arranged by size: 

                4840     202      94
 
SacI G_AGCT'C

Cuts at:      0     636    2337    2538    3549    3756    3954    5136 
   Size:        636    1701     201    1011     207     198    1182

  Fragments arranged by size: 

                1701    1182    1011     636     207     201     198
 
SacII CC_GC'GG

Cuts at:      0    1413    3588    4986    5136 
   Size:       1413    2175    1398     150

  Fragments arranged by size: 

                2175    1413    1398     150
 
SanDI GG'GwC_CC

Cuts at:      0     745    1003    1219    1803    1961    2607    2741    2913 
   Size:        745     258     216     584     158     646     134     172

Cuts at:   2913    4111    4213    5136 
   Size:       1198     102     923

  Fragments arranged by size: 

                1198     923     745     646     584     258     216     172
                 158     134     102
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1365    3920    5136 
   Size:       1365    2555    1216

  Fragments arranged by size: 

                2555    1365    1216
 
SexAI A'CCwGG_T

Cuts at:      0    3342    3558    3837    3900    4281    5136 
   Size:       3342     216     279      63     381     855

  Fragments arranged by size: 

                3342     855     381     279     216      63
 
SfcI C'TryA_G

Cuts at:      0     247    5136 
   Size:        247    4889
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     477    1568    1766    1877    1886    1949    2246    2292 
   Size:        477    1091     198     111       9      63     297      46

Cuts at:   2292    2468    2495    5136 
   Size:        176      27    2641

  Fragments arranged by size: 

                2641    1091     477     297     198     176     111      63
                  46      27       9
 
SfoI GGC'GCC

Cuts at:      0    3854    5136 
   Size:       3854    1282
 
SmaI CCC'GGG

Cuts at:      0     242     368     477    1000    1094    1148    1311    1704 
   Size:        242     126     109     523      94      54     163     393

Cuts at:   1704    2343    2748    2820    4146    4164    4427    5136 
   Size:        639     405      72    1326      18     263     709

  Fragments arranged by size: 

                1326     709     639     523     405     393     263     242
                 163     126     109      94      72      54      18
 
SmlI C'TyrA_G

Cuts at:      0     924    1591    5077    5136 
   Size:        924     667    3486      59

  Fragments arranged by size: 

                3486     924     667      59
 
SphI G_CATG'C

Cuts at:      0    2799    5136 
   Size:       2799    2337
 
SrfI GCCC'GGGC

Cuts at:      0    2343    4427    5136 
   Size:       2343    2084     709

  Fragments arranged by size: 

                2343    2084     709
 
StuI AGG'CCT

Cuts at:      0     968    1202    2069    2105    2390    2471    2498    3236 
   Size:        968     234     867      36     285      81      27     738

Cuts at:   3236    3311    4008    5136 
   Size:         75     697    1128

  Fragments arranged by size: 

                1128     968     867     738     697     285     234      81
                  75      36      27
 
StyI C'CwwG_G

Cuts at:      0     302     483    1007    1110    1223    1502    2054    2430 
   Size:        302     181     524     103     113     279     552     376

Cuts at:   2430    2555    2790    3030    3423    3611    3747    4106    4115 
   Size:        125     235     240     393     188     136     359       9

Cuts at:   4115    4317    5136 
   Size:        202     819

  Fragments arranged by size: 

                 819     552     524     393     376     359     302     279
                 240     235     202     188     181     136     125     113
                 103       9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     824    4832    5136 
   Size:        824    4008     304

  Fragments arranged by size: 

                4008     824     304
 
TatI w'GTAC_w

Cuts at:      0     818    1453    4553    4640    5012    5136 
   Size:        818     635    3100      87     372     124

  Fragments arranged by size: 

                3100     818     635     372     124      87
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     456     488    1698    1730    1919    1951    2610    2642 
   Size:        456      32    1210      32     189      32     659      32

Cuts at:   2642    4492    4524    5136 
   Size:       1850      32     612

  Fragments arranged by size: 

                1850    1210     659     612     456     189      32      32
                  32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     179    5136 
   Size:        179    4957
 
XbaI T'CTAG_A

Cuts at:      0    4938    5136 
   Size:       4938     198
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     211     309    2232    2437    2797    3430    4006    4086 
   Size:        211      98    1923     205     360     633     576      80

Cuts at:   4086    4324    4387    4512    4728    5136 
   Size:        238      63     125     216     408

  Fragments arranged by size: 

                1923     633     576     408     360     238     216     211
                 205     125      98      80      63
 
XmaI C'CCGG_G

Cuts at:      0     240     366     475     998    1092    1146    1309    1702 
   Size:        240     126     109     523      94      54     163     393

Cuts at:   1702    2341    2746    2818    4144    4162    4425    5136 
   Size:        639     405      72    1326      18     263     711

  Fragments arranged by size: 

                1326     711     639     523     405     393     263     240
                 163     126     109      94      72      54      18
 
XmnI GAAnn'nnTTC

Cuts at:      0    3355    5136 
   Size:       3355    1781
 

 Enzymes that do cut:

     AarI     AcuI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BciVI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BpmI    BpuEI     BsaI
    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII   BstAPI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI    HaeII
    Hin4I     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
   NgoMIV     NspI     PasI    PflMI     PfoI    PpuMI    PshAI   PspOMI
     PsrI     PstI    PvuII     SacI    SacII    SanDI     SapI    SexAI
     SfcI     SfiI     SfoI     SmaI     SmlI     SphI     SrfI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AlfI     AscI     AseI    AsiSI    AvrII     BaeI    BbvCI     BcgI
     BclI    BfrBI    BglII     BmtI     BplI   Bpu10I    BsaAI    BsaWI
    BsiWI    BspEI    BspHI    BssSI    BstBI  BstZ17I   Bsu36I     ClaI
    CspCI     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PciI     PmeI     PmlI     PpiI     PsiI    PspXI     PvuI    RsrII
     SalI     SbfI     ScaI     SgfI    SgrAI    SnaBI     SpeI     SspI
     SwaI     XhoI     ZraI
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