Restriction map for
pF1KB9023
(Linear) (Six-base) MAPSORT of: KIBB9023.seq Check: 1852 from: 1 to: 4125
KIBB9023.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1813 2550 4125
Size: 1813 737 1575
Fragments arranged by size:
1813 1575 737
AccI GT'mk_AC
Cuts at: 0 1196 3252 4097 4125
Size: 1196 2056 845 28
Fragments arranged by size:
2056 1196 845 28
Acc65I G'GTAC_C
Cuts at: 0 2372 2977 4125
Size: 2372 605 1148
Fragments arranged by size:
2372 1148 605
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 724 882 1337 1340 1595 1797 3367
Size: 640 84 158 455 3 255 202 1570
Cuts at: 3367 3584 3939 4125
Size: 217 355 186
Fragments arranged by size:
1570 640 455 355 255 217 202 186
158 84 3
AfeI AGC'GCT
Cuts at: 0 3061 4125
Size: 3061 1064
AflII C'TTAA_G
Cuts at: 0 1371 4125
Size: 1371 2754
AflIII A'CryG_T
Cuts at: 0 2303 4125
Size: 2303 1822
AhdI GACnn_n'nnGTC
Cuts at: 0 3925 4125
Size: 3925 200
AleI CACnn'nnGTG
Cuts at: 0 1651 2275 2306 3089 4125
Size: 1651 624 31 783 1036
Fragments arranged by size:
1651 1036 783 624 31
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2733 2852 2923 3226 3343 4125
Size: 2733 119 71 303 117 782
Fragments arranged by size:
2733 782 303 119 117 71
AlwNI CAG_nnn'CTG
Cuts at: 0 141 1828 2169 3529 4125
Size: 141 1687 341 1360 596
Fragments arranged by size:
1687 1360 596 341 141
ApaI G_GGCC'C
Cuts at: 0 1129 2868 4125
Size: 1129 1739 1257
Fragments arranged by size:
1739 1257 1129
ApaLI G'TGCA_C
Cuts at: 0 1639 2667 4125
Size: 1639 1028 1458
Fragments arranged by size:
1639 1458 1028
ApoI r'AATT_y
Cuts at: 0 1971 3543 3797 4125
Size: 1971 1572 254 328
Fragments arranged by size:
1971 1572 328 254
AvaI C'yCGr_G
Cuts at: 0 525 668 1863 3101 4125
Size: 525 143 1195 1238 1024
Fragments arranged by size:
1238 1195 1024 525 143
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1222 1255 4125
Size: 1222 33 2870
Fragments arranged by size:
2870 1222 33
BamHI G'GATC_C
Cuts at: 0 782 1083 3708 4125
Size: 782 301 2625 417
Fragments arranged by size:
2625 782 417 301
BanI G'GyrC_C
Cuts at: 0 232 1953 2324 2372 2660 2977 3094 3217
Size: 232 1721 371 48 288 317 117 123
Cuts at: 3217 3235 3319 3373 4125
Size: 18 84 54 752
Fragments arranged by size:
1721 752 371 317 288 232 123 117
84 54 48 18
BanII G_rGCy'C
Cuts at: 0 107 1129 1154 2868 3032 4125
Size: 107 1022 25 1714 164 1093
Fragments arranged by size:
1714 1093 1022 164 107 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 1409 1422 4125
Size: 1409 13 2703
Fragments arranged by size:
2703 1409 13
BbvCI CC'TCA_GC
Cuts at: 0 1898 4125
Size: 1898 2227
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 174 208 4125
Size: 174 34 3917
Fragments arranged by size:
3917 174 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1044 4125
Size: 1044 3081
BclI T'GATC_A
Cuts at: 0 2531 3803 4125
Size: 2531 1272 322
Fragments arranged by size:
2531 1272 322
BglI GCCn_nnn'nGGC
Cuts at: 0 469 1699 1769 4125
Size: 469 1230 70 2356
Fragments arranged by size:
2356 1230 469 70
BglII A'GATC_T
Cuts at: 0 3678 4125
Size: 3678 447
BlpI GC'TnA_GC
Cuts at: 0 165 4125
Size: 165 3960
Bme1580I G_kGCm'C
Cuts at: 0 235 1129 1643 2663 2671 2868 3122 3220
Size: 235 894 514 1020 8 197 254 98
Cuts at: 3220 3322 4125
Size: 102 803
Fragments arranged by size:
1020 894 803 514 254 235 197 102
98 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 2841 3117 3526 4125
Size: 2841 276 409 599
Fragments arranged by size:
2841 599 409 276
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 399 4125
Size: 399 3726
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 809 3084 3100 3913 4017 4125
Size: 809 2275 16 813 104 108
Fragments arranged by size:
2275 813 809 108 104 16
Bpu10I CC'TnA_GC
Cuts at: 0 1898 1924 4125
Size: 1898 26 2201
Fragments arranged by size:
2201 1898 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2787 3928 4125
Size: 2787 1141 197
Fragments arranged by size:
2787 1141 197
BsaI GGTCTCn'nnnn_
Cuts at: 0 848 1162 4125
Size: 848 314 2963
Fragments arranged by size:
2963 848 314
BsaAI yAC'GTr
Cuts at: 0 228 1436 1623 3117 4125
Size: 228 1208 187 1494 1008
Fragments arranged by size:
1494 1208 1008 228 187
BsaBI GATnn'nnATC
Cuts at: 0 4052 4125
Size: 4052 73
BsaWI w'CCGG_w
Cuts at: 0 2274 4125
Size: 2274 1851
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1268 1298 1999 2005 2029 2035 2562 2592
Size: 1268 30 701 6 24 6 527 30
Cuts at: 2592 2970 3000 3249 3279 3993 4023 4125
Size: 378 30 249 30 714 30 102
Fragments arranged by size:
1268 714 701 527 378 249 102 30
30 30 30 30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 514 979 1012 1729 1885 4014 4125
Size: 514 465 33 717 156 2129 111
Fragments arranged by size:
2129 717 514 465 156 111 33
BseYI C'CCAG_C
Cuts at: 0 396 1179 1308 1965 2162 2584 3227 3866
Size: 396 783 129 657 197 422 643 639
Cuts at: 3866 4125
Size: 259
Fragments arranged by size:
783 657 643 639 422 396 259 197
129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 178 1485 2145 3232 4125
Size: 76 102 1307 660 1087 893
Fragments arranged by size:
1307 1087 893 660 102 76
BsiEI CG_ry'CG
Cuts at: 0 28 3539 4125
Size: 28 3511 586
Fragments arranged by size:
3511 586 28
BsiHKAI G_wGCw'C
Cuts at: 0 107 1643 2580 2671 3086 4125
Size: 107 1536 937 91 415 1039
Fragments arranged by size:
1536 1039 937 415 107 91
BsmI GAATG_Cn'
Cuts at: 0 2689 4125
Size: 2689 1436
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1867 3526 4125
Size: 1867 1659 599
Fragments arranged by size:
1867 1659 599
Bsp1286I G_dGCh'C
Cuts at: 0 107 235 1129 1154 1643 2580 2663 2671
Size: 107 128 894 25 489 937 83 8
Cuts at: 2671 2868 3032 3086 3122 3220 3322 4125
Size: 197 164 54 36 98 102 803
Fragments arranged by size:
937 894 803 489 197 164 128 107
102 98 83 54 36 25 8
BspHI T'CATG_A
Cuts at: 0 2964 4125
Size: 2964 1161
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 474 1667 1813 2550 2725 3083 4125
Size: 474 1193 146 737 175 358 1042
Fragments arranged by size:
1193 1042 737 474 358 175 146
BsrBI CCG'CTC
Cuts at: 0 3224 4125
Size: 3224 901
BsrDI GCAATG_nn'
Cuts at: 0 1686 4125
Size: 1686 2439
BsrFI r'CCGG_y
Cuts at: 0 27 2513 2650 4125
Size: 27 2486 137 1475
Fragments arranged by size:
2486 1475 137 27
BsrGI T'GTAC_A
Cuts at: 0 3898 4125
Size: 3898 227
BssHII G'CGCG_C
Cuts at: 0 420 442 4125
Size: 420 22 3683
Fragments arranged by size:
3683 420 22
BssSI C'ACGA_G
Cuts at: 0 342 4125
Size: 342 3783
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1569 1828 4125
Size: 1569 259 2297
Fragments arranged by size:
2297 1569 259
BstBI TT'CG_AA
Cuts at: 0 1338 4125
Size: 1338 2787
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2383 3352 3432 3705 3891 4125
Size: 2383 969 80 273 186 234
Fragments arranged by size:
2383 969 273 234 186 80
BstYI r'GATC_y
Cuts at: 0 782 1083 3678 3708 4125
Size: 782 301 2595 30 417
Fragments arranged by size:
2595 782 417 301 30
BstZ17I GTA'TAC
Cuts at: 0 3253 4125
Size: 3253 872
BtgI C'CryG_G
Cuts at: 0 83 170 348 2607 3162 3272 4125
Size: 83 87 178 2259 555 110 853
Fragments arranged by size:
2259 853 555 178 110 87 83
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 300 2413 2669 3143 3684 4125
Size: 300 2113 256 474 541 441
Fragments arranged by size:
2113 541 474 441 300 256
BtsI GCAGTG_nn'
Cuts at: 0 398 1530 3163 4125
Size: 398 1132 1633 962
Fragments arranged by size:
1633 1132 962 398
DraI TTT'AAA
Cuts at: 0 1136 4125
Size: 1136 2989
DraIII CAC_nnn'GTG
Cuts at: 0 1593 2818 4125
Size: 1593 1225 1307
Fragments arranged by size:
1593 1307 1225
DrdI GACnn_nn'nnGTC
Cuts at: 0 3408 4125
Size: 3408 717
EaeI y'GGCC_r
Cuts at: 0 17 25 311 404 470 1011 1611 1700
Size: 17 8 286 93 66 541 600 89
Cuts at: 1700 3292 4035 4125
Size: 1592 743 90
Fragments arranged by size:
1592 743 600 541 286 93 90 89
66 17 8
EagI C'GGCC_G
Cuts at: 0 25 4125
Size: 25 4100
EarI CTCTTCn'nnn_
Cuts at: 0 112 879 3673 4125
Size: 112 767 2794 452
Fragments arranged by size:
2794 767 452 112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 346 4125
Size: 346 3779
EcoICRI GAG'CTC
Cuts at: 0 105 4125
Size: 105 4020
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 724 809 882 1337 1340 1595 1797
Size: 640 84 85 73 455 3 255 202
Cuts at: 1797 3084 3100 3367 3584 3913 3939 4017 4125
Size: 1287 16 267 217 329 26 78 108
Fragments arranged by size:
1287 640 455 329 267 255 217 202
108 85 84 78 73 26 16 3
EcoO109I rG'GnC_Cy
Cuts at: 0 1125 1734 2864 3041 3663 4125
Size: 1125 609 1130 177 622 462
Fragments arranged by size:
1130 1125 622 609 462 177
EcoRI G'AATT_C
Cuts at: 0 3543 3797 4125
Size: 3543 254 328
Fragments arranged by size:
3543 328 254
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3192 3684 3969 4125
Size: 3192 492 285 156
Fragments arranged by size:
3192 492 285 156
FspI TGC'GCA
Cuts at: 0 2364 4125
Size: 2364 1761
FspAI rTGC'GCAy
Cuts at: 0 2364 4125
Size: 2364 1761
HaeII r_GCGC'y
Cuts at: 0 91 494 520 2835 3063 3638 4125
Size: 91 403 26 2315 228 575 487
Fragments arranged by size:
2315 575 487 403 228 91 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 148 180 347 379 691 723 2350 2382
Size: 148 32 167 32 312 32 1627 32
Cuts at: 2382 2671 2703 2785 2817 3479 3511 3835 3867
Size: 289 32 82 32 662 32 324 32
Cuts at: 3867 3911 3943 4061 4093 4125
Size: 44 32 118 32 32
Fragments arranged by size:
1627 662 324 312 289 167 148 118
82 44 32 32 32 32 32 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 2755 4125
Size: 2755 1370
KpnI G_GTAC'C
Cuts at: 0 2376 2981 4125
Size: 2376 605 1144
Fragments arranged by size:
2376 1144 605
MfeI C'AATT_G
Cuts at: 0 1754 4125
Size: 1754 2371
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 806 831 1442 2042 4125
Size: 806 25 611 600 2083
Fragments arranged by size:
2083 806 611 600 25
MscI TGG'CCA
Cuts at: 0 472 1013 1613 4037 4125
Size: 472 541 600 2424 88
Fragments arranged by size:
2424 600 541 472 88
MslI CAynn'nnrTG
Cuts at: 0 1651 2275 2306 2432 2573 3089 3430 3703
Size: 1651 624 31 126 141 516 341 273
Cuts at: 3703 4125
Size: 422
Fragments arranged by size:
1651 624 516 422 341 273 141 126
31
MspA1I CmG'CkG
Cuts at: 0 85 844 865 2169 2627 4125
Size: 85 759 21 1304 458 1498
Fragments arranged by size:
1498 1304 759 458 85 21
NaeI GCC'GGC
Cuts at: 0 2652 4125
Size: 2652 1473
NcoI C'CATG_G
Cuts at: 0 2607 3272 4125
Size: 2607 665 853
Fragments arranged by size:
2607 853 665
NgoMIV G'CCGG_C
Cuts at: 0 2650 4125
Size: 2650 1475
NspI r_CATG'y
Cuts at: 0 1495 1666 2307 4125
Size: 1495 171 641 1818
Fragments arranged by size:
1818 1495 641 171
PasI CC'CwG_GG
Cuts at: 0 266 885 1154 2860 4125
Size: 266 619 269 1706 1265
Fragments arranged by size:
1706 1265 619 269 266
PciI A'CATG_T
Cuts at: 0 2303 4125
Size: 2303 1822
PflMI CCAn_nnn'nTGG
Cuts at: 0 1845 4125
Size: 1845 2280
PfoI T'CCnGG_A
Cuts at: 0 3617 4125
Size: 3617 508
PmlI CAC'GTG
Cuts at: 0 1623 4125
Size: 1623 2502
PpuMI rG'GwC_Cy
Cuts at: 0 3663 4125
Size: 3663 462
PshAI GACnn'nnGTC
Cuts at: 0 2145 4125
Size: 2145 1980
PspOMI G'GGCC_C
Cuts at: 0 1125 2864 4125
Size: 1125 1739 1261
Fragments arranged by size:
1739 1261 1125
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2098 2130 4125
Size: 2098 32 1995
Fragments arranged by size:
2098 1995 32
PstI C_TGCA'G
Cuts at: 0 65 146 614 849 2712 3158 4095 4125
Size: 65 81 468 235 1863 446 937 30
Fragments arranged by size:
1863 937 468 446 235 81 65 30
PvuI CG_AT'CG
Cuts at: 0 3539 4125
Size: 3539 586
PvuII CAG'CTG
Cuts at: 0 844 2169 2627 4125
Size: 844 1325 458 1498
Fragments arranged by size:
1498 1325 844 458
SacI G_AGCT'C
Cuts at: 0 107 4125
Size: 107 4018
SacII CC_GC'GG
Cuts at: 0 86 4125
Size: 86 4039
SapI GCTCTTCn'nnn_
Cuts at: 0 112 4125
Size: 112 4013
SbfI CC_TGCA'GG
Cuts at: 0 146 4125
Size: 146 3979
SexAI A'CCwGG_T
Cuts at: 0 2117 4125
Size: 2117 2008
SfcI C'TryA_G
Cuts at: 0 61 142 610 845 908 2472 2708 2914
Size: 61 81 468 235 63 1564 236 206
Cuts at: 2914 3154 4091 4125
Size: 240 937 34
Fragments arranged by size:
1564 937 468 240 236 235 206 81
63 61 34
SmaI CCC'GGG
Cuts at: 0 1865 3103 4125
Size: 1865 1238 1022
Fragments arranged by size:
1865 1238 1022
SmlI C'TyrA_G
Cuts at: 0 1371 2802 3943 4125
Size: 1371 1431 1141 182
Fragments arranged by size:
1431 1371 1141 182
SphI G_CATG'C
Cuts at: 0 1495 1666 4125
Size: 1495 171 2459
Fragments arranged by size:
2459 1495 171
StuI AGG'CCT
Cuts at: 0 1763 4125
Size: 1763 2362
StyI C'CwwG_G
Cuts at: 0 618 1079 2607 3272 4125
Size: 618 461 1528 665 853
Fragments arranged by size:
1528 853 665 618 461
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 110 472 2176 3049 3285 4125
Size: 110 362 1704 873 236 840
Fragments arranged by size:
1704 873 840 362 236 110
TatI w'GTAC_w
Cuts at: 0 3467 3599 3728 3898 4125
Size: 3467 132 129 170 227
Fragments arranged by size:
3467 227 170 132 129
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1068 1100 1268 1300 1522 1554 1593 1625
Size: 1068 32 168 32 222 32 39 32
Cuts at: 1625 3249 3260 3281 3292 3597 3629 3993 4025
Size: 1624 11 21 11 305 32 364 32
Cuts at: 4025 4125
Size: 100
Fragments arranged by size:
1624 1068 364 305 222 168 100 39
32 32 32 32 32 32 21 11
11
Tth111I GACn'n_nGTC
Cuts at: 0 832 2182 3613 4125
Size: 832 1350 1431 512
Fragments arranged by size:
1431 1350 832 512
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2989 4125
Size: 2989 1136
XmaI C'CCGG_G
Cuts at: 0 1863 3101 4125
Size: 1863 1238 1024
Fragments arranged by size:
1863 1238 1024
XmnI GAAnn'nnTTC
Cuts at: 0 541 980 1331 1810 4125
Size: 541 439 351 479 2315
Fragments arranged by size:
2315 541 479 439 351
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflII AflIII AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BaeI
BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII KpnI
MfeI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmlI PpuMI PshAI
PspOMI PsrI PstI PvuI PvuII SacI SacII SapI
SbfI SexAI SfcI SmaI SmlI SphI StuI StyI
TaqII TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AgeI AloI AscI AseI AsiSI AvrII
BbeI BfrBI BmgBI BmtI BsaHI BsiWI BspEI BstEII
Bsu36I ClaI CspCI EcoNI EcoRV FseI HindIII HpaI
KasI MluI NarI NdeI NheI NotI NruI NsiI
PacI PmeI PpiI PsiI PspXI RsrII SalI SanDI
ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI
SspI SwaI XbaI XhoI ZraI